ACM5
797
Total Contact Pairs
78
Significant Changes
10.14
Max Increase
-9.08
Max Decrease
Interactive snake plot
GPCRdb-style topology. Click the view buttons to recolor residues by different data layers. Toggle contact links to draw significant residue-residue contacts as arcs. Click loop labels (ICL/ECL) to expand or collapse loop regions.
Color convention: blue = active-favoring, red = inactive-favoring.
Top 100 conformational changes
Residue pairs with the largest RRCS changes between active and inactive states. GPCRdb numbering in parentheses. Highlighted rows exceed the significance threshold.
| Rank | Residue 1 | Residue 2 | Active RRCS | Inactive RRCS | ΔRRCS | Magnitude |
|---|---|---|---|---|---|---|
| 1 | LYS330 | GLU331 | 11.445 | 1.307 | +10.138 | HIGH |
| 2 | GLU352 | LYS353 | 9.337 | 0.000 | +9.337 | HIGH |
| 3 | ASN65 (2.39x39) | ARG128 (3.50x50) | 0.000 | 9.077 | -9.077 | HIGH |
| 4 | MET508 (8.56x56) | TRP514 | 0.000 | 9.031 | -9.031 | HIGH |
| 5 | VAL57 (1.59x59) | TRP514 | 0.000 | 8.812 | -8.812 | HIGH |
| 6 | TRP28 (1.30x30) | GLU29 (1.31x31) | 0.000 | 8.773 | -8.773 | HIGH |
| 7 | ASP355 | TYR356 | 8.428 | 0.000 | +8.428 | HIGH |
| 8 | ARG264 | GLU265 | 2.182 | 10.523 | -8.341 | HIGH |
| 9 | GLU310 | ARG429 | 8.177 | 0.000 | +8.177 | HIGH |
| 10 | GLU265 | ARG266 | 7.322 | 0.000 | +7.322 | HIGH |
| 11 | ARG95 (23.49x49) | ASP181 | 2.851 | 9.993 | -7.143 | HIGH |
| 12 | ARG26 (1.28x28) | GLU29 (1.31x31) | 0.062 | 7.058 | -6.996 | HIGH |
| 13 | TYR87 (2.61x60) | TYR481 (7.39x38) | 12.582 | 6.253 | +6.329 | HIGH |
| 14 | ARG139 (34.54x54) | GLU437 (6.30x30) | 0.000 | 5.696 | -5.696 | HIGH |
| 15 | GLN391 | GLU392 | 1.074 | 6.578 | -5.505 | HIGH |
| 16 | ALA496 (7.54x54) | ARG503 (8.51x51) | 0.000 | 5.489 | -5.489 | HIGH |
| 17 | THR224 (5.69x69) | GLU437 (6.30x30) | 5.390 | 0.000 | +5.390 | HIGH |
| 18 | GLN231 | ARG431 (6.24x24) | 0.000 | 5.389 | -5.389 | HIGH |
| 19 | THR177 | GLN186 | 1.939 | 7.092 | -5.153 | HIGH |
| 20 | LYS517 | GLU521 | 1.461 | 6.552 | -5.091 | HIGH |
| 21 | PHE346 | LYS348 | 0.000 | 5.090 | -5.090 | HIGH |
| 22 | VAL472 | TRP477 (7.35x34) | 0.493 | 5.407 | -4.914 | MED |
| 23 | LEU121 (3.43x43) | TYR495 (7.53x53) | 4.912 | 0.000 | +4.912 | MED |
| 24 | SER100 (3.22x22) | ARG176 | 8.914 | 4.166 | +4.748 | MED |
| 25 | SER270 | TRP271 | 4.491 | 0.000 | +4.491 | MED |
| 26 | THR443 (6.36x36) | CYS498 (7.56x56) | 0.694 | 5.036 | -4.342 | MED |
| 27 | LEU69 (2.43x43) | TYR495 (7.53x53) | 1.331 | 5.513 | -4.182 | MED |
| 28 | ALA496 (7.54x54) | PHE502 (8.50x50) | 4.082 | 0.000 | +4.082 | MED |
| 29 | MET508 (8.56x56) | ARG513 | 0.260 | 4.292 | -4.032 | MED |
| 30 | ASN58 (1.60x60) | TRP514 | 0.000 | 4.024 | -4.024 | MED |
| 31 | PHE202 (5.47x47) | ASN459 (6.52x52) | 9.024 | 13.047 | -4.023 | MED |
| 32 | TYR458 (6.51x51) | TYR481 (7.39x38) | 5.624 | 1.629 | +3.994 | MED |
| 33 | ARG266 | ASN267 | 3.982 | 0.000 | +3.982 | MED |
| 34 | ARG128 (3.50x50) | TYR495 (7.53x53) | 3.958 | 0.000 | +3.958 | MED |
| 35 | LEU50 (1.52x52) | PHE506 (8.54x54) | 6.571 | 2.653 | +3.918 | MED |
| 36 | TYR90 (2.64x63) | CYS183 (45.50x50) | 1.260 | 5.037 | -3.777 | MED |
| 37 | LEU188 | THR194 (5.39x40) | 3.114 | 6.841 | -3.727 | MED |
| 38 | TYR213 (5.58x58) | LEU444 (6.37x37) | 2.059 | 5.769 | -3.710 | MED |
| 39 | TRP514 | LYS518 | 0.000 | 3.645 | -3.645 | MED |
| 40 | TYR87 (2.61x60) | TRP482 (7.40x39) | 6.095 | 2.498 | +3.597 | MED |
| 41 | GLU336 | PHE337 | 4.167 | 0.652 | +3.515 | MED |
| 42 | ASP127 (3.49x49) | ARG128 (3.50x50) | 0.000 | 3.271 | -3.271 | MED |
| 43 | ARG26 (1.28x28) | TRP28 (1.30x30) | 0.000 | 3.260 | -3.260 | MED |
| 44 | LEU161 (4.56x56) | TRP162 (4.57x57) | 3.689 | 0.438 | +3.251 | MED |
| 45 | GLU190 (5.35x36) | THR192 (5.37x38) | 0.000 | 3.251 | -3.251 | MED |
| 46 | TYR356 | ASP357 | 4.252 | 1.034 | +3.219 | MED |
| 47 | PRO492 (7.50x50) | LEU497 (7.55x55) | 3.102 | 0.000 | +3.102 | MED |
| 48 | HIS25 | GLU29 (1.31x31) | 4.386 | 1.309 | +3.077 | MED |
| 49 | PHE451 (6.44x44) | TRP455 (6.48x48) | 5.441 | 8.498 | -3.057 | MED |
| 50 | ASN487 (7.45x45) | ASN491 (7.49x49) | 3.887 | 0.846 | +3.041 | MED |
| 51 | TRP455 (6.48x48) | ASN487 (7.45x45) | 5.644 | 2.711 | +2.933 | MED |
| 52 | ASN65 (2.39x39) | ARG142 | 6.457 | 3.550 | +2.907 | MED |
| 53 | THR224 (5.69x69) | LEU434 | 0.000 | 2.854 | -2.854 | MED |
| 54 | ASN115 | ALA201 | 3.064 | 0.275 | +2.789 | MED |
| 55 | THR358 | PRO359 | 3.569 | 0.831 | +2.737 | MED |
| 56 | PHE195 | PHE467 | 3.512 | 0.803 | +2.709 | MED |
| 57 | ARG223 | LEU434 | 0.000 | 2.693 | -2.693 | MED |
| 58 | LYS238 | HIS424 | 0.000 | 2.574 | -2.574 | MED |
| 59 | MET508 (8.56x56) | LYS517 | 0.000 | 2.568 | -2.568 | MED |
| 60 | SER277 | THR278 | 0.000 | 2.557 | -2.557 | MED |
| 61 | ASP76 | SER488 | 10.113 | 7.561 | +2.552 | MED |
| 62 | PHE130 | TYR138 | 11.382 | 8.832 | +2.550 | MED |
| 63 | GLN170 | LYS175 | 2.292 | 4.825 | -2.532 | MED |
| 64 | GLU350 | THR351 | 4.129 | 1.605 | +2.524 | MED |
| 65 | TRP96 | TRP106 | 4.123 | 6.646 | -2.523 | MED |
| 66 | GLU219 | ARG223 | 5.423 | 7.937 | -2.514 | MED |
| 67 | SER327 | GLN328 | 2.508 | 0.000 | +2.508 | MED |
| 68 | PRO179 | GLU182 | 2.745 | 5.244 | -2.498 | MED |
| 69 | LEU22 | GLY94 | 0.000 | 2.490 | -2.490 | MED |
| 70 | LYS175 | GLN186 | 0.367 | 2.827 | -2.460 | MED |
| 71 | GLU340 | GLU341 | 2.441 | 0.000 | +2.441 | MED |
| 72 | LEU72 | ASN120 | 3.230 | 0.811 | +2.418 | MED |
| 73 | LYS516 | GLU520 | 3.431 | 1.024 | +2.406 | MED |
| 74 | ARG128 (3.50x50) | ARG142 | 0.000 | 2.404 | -2.404 | MED |
| 75 | ASP313 | LYS504 | 0.000 | 2.397 | -2.397 | MED |
| 76 | PHE187 | LEU188 | 0.000 | 2.366 | -2.366 | MED |
| 77 | LEU497 (7.55x55) | PHE506 (8.54x54) | 2.340 | 0.000 | +2.340 | MED |
| 78 | THR88 | TRP482 (7.40x39) | 3.756 | 1.423 | +2.334 | MED |
| 79 | TYR90 (2.64x63) | GLU182 | 0.359 | 2.685 | -2.326 | LOW |
| 80 | PHE55 | TYR67 | 2.641 | 4.958 | -2.317 | LOW |
| 81 | LEU227 | ARG431 (6.24x24) | 0.000 | 2.314 | -2.314 | LOW |
| 82 | ILE132 | ARG223 | 4.696 | 7.007 | -2.311 | LOW |
| 83 | GLY47 | PRO492 (7.50x50) | 1.844 | 4.140 | -2.296 | LOW |
| 84 | THR351 | GLU352 | 2.285 | 0.000 | +2.285 | LOW |
| 85 | PRO191 | THR466 | 1.381 | 3.660 | -2.279 | LOW |
| 86 | SER414 | THR415 | 0.000 | 2.261 | -2.261 | LOW |
| 87 | LYS399 | VAL400 | 2.238 | 0.000 | +2.238 | LOW |
| 88 | ASN48 | PRO492 (7.50x50) | 3.090 | 5.318 | -2.228 | LOW |
| 89 | GLU312 | LYS428 | 2.219 | 0.000 | +2.219 | LOW |
| 90 | TYR217 | ALA441 | 2.211 | 0.000 | +2.211 | LOW |
| 91 | TYR213 (5.58x58) | SER445 | 0.000 | 2.180 | -2.180 | LOW |
| 92 | GLU221 | GLU437 (6.30x30) | 2.170 | 0.000 | +2.170 | LOW |
| 93 | THR393 | ASN394 | 2.205 | 4.361 | -2.156 | LOW |
| 94 | VAL377 | TYR379 | 0.389 | 2.538 | -2.149 | LOW |
| 95 | ALA496 (7.54x54) | PHE506 (8.54x54) | 0.000 | 2.144 | -2.144 | LOW |
| 96 | SER131 | ARG139 (34.54x54) | 2.922 | 5.045 | -2.123 | LOW |
| 97 | ARG128 (3.50x50) | LEU444 (6.37x37) | 0.000 | 2.082 | -2.082 | LOW |
| 98 | ALA339 | GLU340 | 2.080 | 0.000 | +2.080 | LOW |
| 99 | THR224 (5.69x69) | VAL433 | 2.046 | 0.000 | +2.046 | LOW |
| 100 | ASN421 | SER423 | 3.938 | 1.902 | +2.037 | LOW |
Transmembrane domain analysis
Active-favoring residues form stronger contacts in the active state (positive ΔRRCS). Inactive-favoring residues form stronger contacts in the inactive state (negative ΔRRCS). Only residues with |ΔRRCS| ≥ 2.33 are shown (per-receptor significance threshold = max(mean(|Δ|) + σ, 0.2)).
| Segment | Range | Active-favoring residues | Count | Inactive-favoring residues | Count |
|---|---|---|---|---|---|
| TM1 | 26-58 | 50 (3.9) | 1 | 57 (-8.8), 28 (-8.8), 29 (-8.8), 26 (-7.0), 58 (-4.0) | 5 |
| TM2 | 65-90 | 87 (6.3) | 1 | 65 (-9.1), 69 (-4.2), 90 (-3.8) | 3 |
| TM3 | 100-128 | 121 (4.9), 100 (4.7) | 2 | 128 (-9.1), 127 (-3.3) | 2 |
| TM4 | 161-162 | 161 (3.3), 162 (3.3) | 2 | - | 0 |
| TM5 | 190-224 | 224 (5.4) | 1 | 202 (-4.0), 194 (-3.7), 213 (-3.7), 190 (-3.3), 192 (-3.3) | 5 |
| TM6 | 431-459 | 458 (4.0) | 1 | 437 (-5.7), 431 (-5.4), 443 (-4.3), 459 (-4.0), 444 (-3.7), 451 (-3.1), 455 (-3.1) | 7 |
| TM7 | 477-498 | 481 (6.3), 495 (4.9), 482 (3.6), 492 (3.1), 497 (3.1), 487 (3.0), 491 (3.0) | 7 | 496 (-5.5), 477 (-4.9), 498 (-4.3) | 3 |
| Intracellular / Extracellular loops & H8 | |||||
| ICL1 | - | - | 0 | - | 0 |
| ICL2 | 139-139 | - | 0 | 139 (-5.7) | 1 |
| ICL3 | 231-429 | 330 (10.1), 331 (10.1), 352 (9.3), 353 (9.3), 355 (8.4), 356 (8.4), 310 (8.2), 429 (8.2), 266 (7.3), 270 (4.5), 271 (4.5), 267 (4.0), 336 (3.5), 337 (3.5), 357 (3.2) | 15 | 264 (-8.3), 265 (-8.3), 391 (-5.5), 392 (-5.5), 231 (-5.4), 346 (-5.1), 348 (-5.1) | 7 |
| ECL1 | 95-95 | - | 0 | 95 (-7.1) | 1 |
| ECL2 | 176-188 | 176 (4.7) | 1 | 181 (-7.1), 177 (-5.2), 186 (-5.2), 183 (-3.8), 188 (-3.7) | 5 |
| ECL3 | 472-472 | - | 0 | 472 (-4.9) | 1 |
| H8 | 502-508 | 502 (4.1), 506 (3.9) | 2 | 508 (-9.0), 503 (-5.5) | 2 |
Interactive visualizations
ΔRRCS distribution
Active vs inactive comparison
Residue-wise changes
TM domain breakdown
Variants of interest
81 variants mapped to contact positions, sorted by |ΔRRCS| impact.
| Position | Protein change | DNA change | Allele freq | Het / Hom | ΔRRCS | AM score | Pathogenicity | Conservation | dbSNP |
|---|---|---|---|---|---|---|---|---|---|
| 330 | p.Lys330Glu | c.988A>G | 6.84e-07 | 1 / 0 | 10.14 | 0.074 | BENIGN | 0.40 | rs1245948944 |
| 330 | p.Lys330Arg | c.989A>G | 2.05e-06 | 3 / 0 | 10.14 | - | nan | - | rs747242630 |
| 353 | p.Lys353Arg | c.1058A>G | 2.51e-04 | 367 / 0 | 9.34 | 0.078 | BENIGN | 0.34 | rs150113487 |
| 352 | p.Glu352Lys | c.1054G>A | 6.84e-07 | 1 / 0 | 9.34 | 0.065 | BENIGN | 0.38 | |
| 128 | p.Arg128Cys | c.382C>T | 9.58e-06 | 14 / 0 | 9.08 | 0.991 | PATHOGENIC | 1.00 | rs766642500 |
| 65 | p.Asn65Ser | c.194A>G | 6.57e-06 | 1 / 0 | 9.08 | 0.199 | BENIGN | 0.68 | rs1309073991 |
| 128 | p.Arg128His | c.383G>A | 6.16e-06 | 9 / 0 | 9.08 | - | nan | - | rs752179210 |
| 514 | p.Trp514Arg | c.1540T>C | 1.37e-06 | 2 / 0 | 9.03 | 0.853 | PATHOGENIC | 0.59 | rs1362521607 |
| 508 | p.Met508Val | c.1522A>G | 6.84e-07 | 1 / 0 | 9.03 | 0.159 | BENIGN | 0.37 | |
| 514 | p.Trp514Arg | c.1540T>A | 6.84e-07 | 1 / 0 | 9.03 | - | nan | - | rs1362521607 |
| 57 | p.Val57Ile | c.169G>A | 6.84e-07 | 1 / 0 | 8.81 | 0.128 | BENIGN | 0.71 | rs1165544868 |
| 29 | p.Glu29Lys | c.85G>A | 6.84e-07 | 1 / 0 | 8.77 | 0.315 | BENIGN | 0.61 | rs746417976 |
| 355 | p.Asp355His | c.1063G>C | 1.37e-06 | 2 / 0 | 8.43 | 0.096 | BENIGN | 0.33 | rs750302868 |
| 356 | p.Tyr356Asp | c.1066T>G | 6.84e-07 | 1 / 0 | 8.43 | 0.087 | BENIGN | 0.39 | |
| 355 | p.Asp355Gly | c.1064A>G | 6.84e-07 | 1 / 0 | 8.43 | - | nan | - | rs138383914 |
| 356 | p.Tyr356Cys | c.1067A>G | 6.84e-07 | 1 / 0 | 8.43 | - | nan | - | rs1214012533 |
| 264 | p.Arg264Trp | c.790C>T | 5.47e-06 | 8 / 0 | 8.34 | 0.128 | BENIGN | 0.37 | rs764541495 |
| 265 | p.Glu265Gln | c.793G>C | 6.84e-07 | 1 / 0 | 8.34 | 0.091 | BENIGN | 0.41 | |
| 264 | p.Arg264Gln | c.791G>A | 5.47e-06 | 8 / 0 | 8.34 | - | nan | - | rs777128975 |
| 429 | p.Arg429Gly | c.1285C>G | 2.05e-06 | 3 / 0 | 8.18 | 0.546 | AMBIGUOUS | 0.67 | rs772971999 |
| 310 | p.Glu310Ala | c.929A>C | 8.89e-06 | 13 / 0 | 8.18 | 0.138 | BENIGN | 0.43 | rs765851212 |
| 310 | p.Glu310Asp | c.930G>T | 2.74e-06 | 4 / 0 | 8.18 | - | nan | - | |
| 429 | p.Arg429Gln | c.1286G>A | 6.16e-06 | 9 / 0 | 8.18 | - | nan | - | rs762689264 |
| 266 | p.Arg266Lys | c.797G>A | 6.57e-06 | 1 / 0 | 7.32 | 0.077 | BENIGN | 0.41 | rs762383940 |
| 95 | p.Arg95Cys | c.283C>T | 6.16e-06 | 9 / 0 | 7.14 | 0.169 | BENIGN | 0.48 | rs755710294 |
| 95 | p.Arg95His | c.284G>A | 4.79e-06 | 7 / 0 | 7.14 | - | nan | - | rs1475008854 |
| 26 | p.Arg26Gly | c.76A>G | 6.84e-07 | 1 / 0 | 7.00 | 0.058 | BENIGN | 0.48 | |
| 26 | p.Arg26Thr | c.77G>C | 5.47e-06 | 8 / 0 | 7.00 | - | nan | - | rs1900389096 |
| 26 | p.Arg26Met | c.77G>T | 6.57e-06 | 1 / 0 | 7.00 | - | nan | - | rs1900389096 |
| 481 | p.Tyr481Cys | c.1442A>G | 6.84e-07 | 1 / 0 | 6.33 | 0.960 | PATHOGENIC | 0.95 | |
| 87 | p.Tyr87Cys | c.260A>G | 6.84e-07 | 1 / 0 | 6.33 | 0.898 | PATHOGENIC | 0.79 | |
| 87 | p.Tyr87Phe | c.260A>T | 6.84e-07 | 1 / 0 | 6.33 | - | nan | - | |
| 437 | p.Glu437Gln | c.1309G>C | 6.84e-07 | 1 / 0 | 5.70 | 0.920 | PATHOGENIC | 0.85 | rs756372866 |
| 139 | p.Arg139Trp | c.415C>T | 9.58e-06 | 14 / 0 | 5.70 | 0.872 | PATHOGENIC | 0.69 | rs1288575176 |
| 139 | p.Arg139Leu | c.416G>T | 6.84e-07 | 1 / 0 | 5.70 | - | nan | - | rs372901279 |
| 139 | p.Arg139Gln | c.416G>A | 1.03e-05 | 15 / 0 | 5.70 | - | nan | - | rs372901279 |
| 437 | p.Glu437Lys | c.1309G>A | 2.05e-06 | 3 / 0 | 5.70 | - | nan | - | rs756372866 |
| 437 | p.Glu437Gly | c.1310A>G | 6.84e-07 | 1 / 0 | 5.70 | - | nan | - | |
| 437 | p.Glu437Asp | c.1311G>C | 6.84e-07 | 1 / 0 | 5.70 | - | nan | - | |
| 437 | p.Glu437Asp | c.1311G>T | 3.42e-06 | 5 / 0 | 5.70 | - | nan | - | rs541846772 |
| 392 | p.Glu392Asp | c.1176G>C | 2.05e-06 | 3 / 0 | 5.50 | 0.077 | BENIGN | 0.38 | |
| 392 | p.Glu392Asp | c.1176G>T | 7.52e-06 | 11 / 0 | 5.50 | - | nan | - | |
| 503 | p.Arg503Ser | c.1509G>T | 1.71e-05 | 25 / 0 | 5.49 | 0.877 | PATHOGENIC | 0.77 | rs752238862 |
| 224 | p.Thr224Ile | c.671C>T | 6.84e-07 | 1 / 0 | 5.39 | 0.600 | PATHOGENIC | 0.39 | |
| 431 | p.Arg431Thr | c.1292G>C | 2.05e-06 | 3 / 0 | 5.39 | 0.412 | AMBIGUOUS | 0.48 | rs774093394 |
| 231 | p.Gln231Arg | c.692A>G | 1.37e-06 | 2 / 0 | 5.39 | 0.148 | BENIGN | 0.62 | |
| 431 | p.Arg431Ser | c.1293A>C | 4.10e-06 | 6 / 0 | 5.39 | - | nan | - | |
| 431 | p.Arg431Ser | c.1293A>T | 6.84e-07 | 1 / 0 | 5.39 | - | nan | - | |
| 186 | p.Gln186Arg | c.557A>G | 2.05e-06 | 3 / 0 | 5.15 | 0.895 | PATHOGENIC | 0.85 | |
| 177 | p.Thr177Ala | c.529A>G | 6.84e-07 | 1 / 0 | 5.15 | 0.204 | BENIGN | 0.71 | rs1900409275 |
| 348 | p.Lys348Glu | c.1042A>G | 2.05e-06 | 3 / 0 | 5.09 | 0.074 | BENIGN | 0.47 | |
| 348 | p.Lys348Asn | c.1044A>T | 6.84e-07 | 1 / 0 | 5.09 | - | nan | - | rs1383585565 |
| 477 | p.Trp477Arg | c.1429T>A | 8.89e-06 | 13 / 0 | 4.91 | 0.987 | PATHOGENIC | 0.75 | rs775687892 |
| 472 | p.Val472Ile | c.1414G>A | 3.42e-06 | 5 / 0 | 4.91 | 0.064 | BENIGN | 0.78 | |
| 495 | p.Tyr495Cys | c.1484A>G | 1.37e-06 | 2 / 0 | 4.91 | 0.996 | PATHOGENIC | 0.95 | rs1186621097 |
| 176 | p.Arg176Trp | c.526C>T | 1.78e-05 | 26 / 0 | 4.75 | 0.804 | PATHOGENIC | 0.84 | rs769941156 |
| 100 | p.Ser100Gly | c.298A>G | 6.16e-06 | 9 / 0 | 4.75 | 0.088 | BENIGN | 0.49 | rs367857442 |
| 176 | p.Arg176Gln | c.527G>A | 6.16e-06 | 9 / 0 | 4.75 | - | nan | - | rs773544114 |
| 271 | p.Trp271Ser | c.812G>C | 6.84e-07 | 1 / 0 | 4.49 | 0.220 | BENIGN | 0.35 | rs767472455 |
| 270 | p.Ser270Cys | c.809C>G | 6.84e-07 | 1 / 0 | 4.49 | 0.145 | BENIGN | 0.43 | rs1345518801 |
| 271 | p.Trp271Cys | c.813G>C | 2.05e-06 | 3 / 0 | 4.49 | - | nan | - | rs752560317 |
| 443 | p.Thr443Ala | c.1327A>G | 3.29e-05 | 5 / 0 | 4.34 | 0.888 | PATHOGENIC | 0.82 | rs930066323 |
| 443 | p.Thr443Ile | c.1328C>T | 2.43e-04 | 355 / 0 | 4.34 | - | nan | - | rs146054258 |
| 513 | p.Arg513Gln | c.1538G>A | 1.51e-05 | 22 / 0 | 4.03 | 0.059 | BENIGN | 0.60 | rs367778274 |
| 58 | p.Asn58His | c.172A>C | 6.84e-07 | 1 / 0 | 4.02 | 0.597 | PATHOGENIC | 0.74 | rs1900394601 |
| 459 | p.Asn459Thr | c.1376A>C | 6.84e-07 | 1 / 0 | 4.02 | 0.961 | PATHOGENIC | 0.78 | |
| 506 | p.Phe506Leu | c.1516T>C | 6.84e-07 | 1 / 0 | 3.92 | 0.996 | PATHOGENIC | 0.89 | |
| 50 | p.Leu50Phe | c.150G>C | 6.84e-06 | 10 / 0 | 3.92 | 0.681 | PATHOGENIC | 0.82 | rs774098229 |
| 188 | p.Leu188Val | c.562C>G | 1.37e-06 | 2 / 0 | 3.73 | 0.572 | PATHOGENIC | 0.70 | |
| 213 | p.Tyr213Cys | c.638A>G | 6.84e-07 | 1 / 0 | 3.71 | 0.959 | PATHOGENIC | 0.95 | |
| 444 | p.Leu444Val | c.1330C>G | 1.37e-06 | 2 / 0 | 3.71 | 0.865 | PATHOGENIC | 0.74 | rs1360152982 |
| 518 | p.Lys518Asn | c.1554A>C | 6.16e-06 | 9 / 0 | 3.64 | 0.249 | BENIGN | 0.71 | rs778538576 |
| 337 | p.Phe337Leu | c.1009T>C | 6.84e-07 | 1 / 0 | 3.51 | 0.324 | BENIGN | 0.30 | rs1270541618 |
| 337 | p.Phe337Cys | c.1010T>G | 1.92e-05 | 28 / 0 | 3.51 | - | nan | - | rs759117995 |
| 337 | p.Phe337Ser | c.1010T>C | 6.84e-07 | 1 / 0 | 3.51 | - | nan | - | |
| 161 | p.Leu161Pro | c.482T>C | 1.37e-06 | 2 / 0 | 3.25 | 0.988 | PATHOGENIC | 0.92 | |
| 192 | p.Thr192Ile | c.575C>T | 1.98e-05 | 29 / 0 | 3.25 | 0.067 | BENIGN | 0.41 | rs768040535 |
| 357 | p.Asp357Asn | c.1069G>A | 2.05e-06 | 3 / 0 | 3.22 | 0.071 | BENIGN | 0.41 | rs1900432468 |
| 492 | p.Pro492Ala | c.1474C>G | 6.84e-07 | 1 / 0 | 3.10 | 0.996 | PATHOGENIC | 0.96 | |
| 491 | p.Asn491Lys | c.1473C>A | 4.10e-06 | 6 / 0 | 3.04 | 1.000 | PATHOGENIC | 0.96 | |
| 487 | p.Asn487Asp | c.1459A>G | 6.57e-06 | 1 / 0 | 3.04 | 0.997 | PATHOGENIC | 0.97 | rs1410981312 |
Complete RRCS results
Top 1000 contact pairs by |ΔRRCS|. Significance threshold: |ΔRRCS| ≥ 2.33, computed as max(mean(|Δ|) + σ, 0.2). Highlighted rows indicate significant changes.
| Res1 | AA1 | Res2 | AA2 | Active RRCS | Inactive RRCS | ΔRRCS | |ΔRRCS| |
|---|---|---|---|---|---|---|---|
| 330 | LYS | 331 | GLU | 11.445 | 1.307 | 10.138 | 10.138 |
| 352 | GLU | 353 | LYS | 9.337 | 0.000 | 9.337 | 9.337 |
| 65 | ASN | 128 | ARG | 0.000 | 9.077 | -9.077 | 9.077 |
| 508 | MET | 514 | TRP | 0.000 | 9.031 | -9.031 | 9.031 |
| 57 | VAL | 514 | TRP | 0.000 | 8.812 | -8.812 | 8.812 |
| 28 | TRP | 29 | GLU | 0.000 | 8.773 | -8.773 | 8.773 |
| 355 | ASP | 356 | TYR | 8.428 | 0.000 | 8.428 | 8.428 |
| 264 | ARG | 265 | GLU | 2.182 | 10.523 | -8.341 | 8.341 |
| 310 | GLU | 429 | ARG | 8.177 | 0.000 | 8.177 | 8.177 |
| 265 | GLU | 266 | ARG | 7.322 | 0.000 | 7.322 | 7.322 |
| 95 | ARG | 181 | ASP | 2.851 | 9.993 | -7.143 | 7.143 |
| 26 | ARG | 29 | GLU | 0.062 | 7.058 | -6.996 | 6.996 |
| 87 | TYR | 481 | TYR | 12.582 | 6.253 | 6.329 | 6.329 |
| 139 | ARG | 437 | GLU | 0.000 | 5.696 | -5.696 | 5.696 |
| 391 | GLN | 392 | GLU | 1.074 | 6.578 | -5.505 | 5.505 |
| 496 | ALA | 503 | ARG | 0.000 | 5.489 | -5.489 | 5.489 |
| 224 | THR | 437 | GLU | 5.390 | 0.000 | 5.390 | 5.390 |
| 231 | GLN | 431 | ARG | 0.000 | 5.389 | -5.389 | 5.389 |
| 177 | THR | 186 | GLN | 1.939 | 7.092 | -5.153 | 5.153 |
| 517 | LYS | 521 | GLU | 1.461 | 6.552 | -5.091 | 5.091 |
| 346 | PHE | 348 | LYS | 0.000 | 5.090 | -5.090 | 5.090 |
| 472 | VAL | 477 | TRP | 0.493 | 5.407 | -4.914 | 4.914 |
| 121 | LEU | 495 | TYR | 4.912 | 0.000 | 4.912 | 4.912 |
| 100 | SER | 176 | ARG | 8.914 | 4.166 | 4.748 | 4.748 |
| 270 | SER | 271 | TRP | 4.491 | 0.000 | 4.491 | 4.491 |
| 443 | THR | 498 | CYS | 0.694 | 5.036 | -4.342 | 4.342 |
| 69 | LEU | 495 | TYR | 1.331 | 5.513 | -4.182 | 4.182 |
| 496 | ALA | 502 | PHE | 4.082 | 0.000 | 4.082 | 4.082 |
| 508 | MET | 513 | ARG | 0.260 | 4.292 | -4.032 | 4.032 |
| 58 | ASN | 514 | TRP | 0.000 | 4.024 | -4.024 | 4.024 |
| 202 | PHE | 459 | ASN | 9.024 | 13.047 | -4.023 | 4.023 |
| 458 | TYR | 481 | TYR | 5.624 | 1.629 | 3.994 | 3.994 |
| 266 | ARG | 267 | ASN | 3.982 | 0.000 | 3.982 | 3.982 |
| 128 | ARG | 495 | TYR | 3.958 | 0.000 | 3.958 | 3.958 |
| 50 | LEU | 506 | PHE | 6.571 | 2.653 | 3.918 | 3.918 |
| 90 | TYR | 183 | CYS | 1.260 | 5.037 | -3.777 | 3.777 |
| 188 | LEU | 194 | THR | 3.114 | 6.841 | -3.727 | 3.727 |
| 213 | TYR | 444 | LEU | 2.059 | 5.769 | -3.710 | 3.710 |
| 514 | TRP | 518 | LYS | 0.000 | 3.645 | -3.645 | 3.645 |
| 87 | TYR | 482 | TRP | 6.095 | 2.498 | 3.597 | 3.597 |
| 336 | GLU | 337 | PHE | 4.167 | 0.652 | 3.515 | 3.515 |
| 127 | ASP | 128 | ARG | 0.000 | 3.271 | -3.271 | 3.271 |
| 26 | ARG | 28 | TRP | 0.000 | 3.260 | -3.260 | 3.260 |
| 161 | LEU | 162 | TRP | 3.689 | 0.438 | 3.251 | 3.251 |
| 190 | GLU | 192 | THR | 0.000 | 3.251 | -3.251 | 3.251 |
| 356 | TYR | 357 | ASP | 4.252 | 1.034 | 3.219 | 3.219 |
| 492 | PRO | 497 | LEU | 3.102 | 0.000 | 3.102 | 3.102 |
| 25 | HIS | 29 | GLU | 4.386 | 1.309 | 3.077 | 3.077 |
| 451 | PHE | 455 | TRP | 5.441 | 8.498 | -3.057 | 3.057 |
| 487 | ASN | 491 | ASN | 3.887 | 0.846 | 3.041 | 3.041 |
| 455 | TRP | 487 | ASN | 5.644 | 2.711 | 2.933 | 2.933 |
| 65 | ASN | 142 | ARG | 6.457 | 3.550 | 2.907 | 2.907 |
| 224 | THR | 434 | LEU | 0.000 | 2.854 | -2.854 | 2.854 |
| 115 | ASN | 201 | ALA | 3.064 | 0.275 | 2.789 | 2.789 |
| 358 | THR | 359 | PRO | 3.569 | 0.831 | 2.737 | 2.737 |
| 195 | PHE | 467 | PHE | 3.512 | 0.803 | 2.709 | 2.709 |
| 223 | ARG | 434 | LEU | 0.000 | 2.693 | -2.693 | 2.693 |
| 238 | LYS | 424 | HIS | 0.000 | 2.574 | -2.574 | 2.574 |
| 508 | MET | 517 | LYS | 0.000 | 2.568 | -2.568 | 2.568 |
| 277 | SER | 278 | THR | 0.000 | 2.557 | -2.557 | 2.557 |
| 76 | ASP | 488 | SER | 10.113 | 7.561 | 2.552 | 2.552 |
| 130 | PHE | 138 | TYR | 11.382 | 8.832 | 2.550 | 2.550 |
| 170 | GLN | 175 | LYS | 2.292 | 4.825 | -2.532 | 2.532 |
| 350 | GLU | 351 | THR | 4.129 | 1.605 | 2.524 | 2.524 |
| 96 | TRP | 106 | TRP | 4.123 | 6.646 | -2.523 | 2.523 |
| 219 | GLU | 223 | ARG | 5.423 | 7.937 | -2.514 | 2.514 |
| 327 | SER | 328 | GLN | 2.508 | 0.000 | 2.508 | 2.508 |
| 179 | PRO | 182 | GLU | 2.745 | 5.244 | -2.498 | 2.498 |
| 22 | LEU | 94 | GLY | 0.000 | 2.490 | -2.490 | 2.490 |
| 175 | LYS | 186 | GLN | 0.367 | 2.827 | -2.460 | 2.460 |
| 340 | GLU | 341 | GLU | 2.441 | 0.000 | 2.441 | 2.441 |
| 72 | LEU | 120 | ASN | 3.230 | 0.811 | 2.418 | 2.418 |
| 516 | LYS | 520 | GLU | 3.431 | 1.024 | 2.406 | 2.406 |
| 128 | ARG | 142 | ARG | 0.000 | 2.404 | -2.404 | 2.404 |
| 313 | ASP | 504 | LYS | 0.000 | 2.397 | -2.397 | 2.397 |
| 187 | PHE | 188 | LEU | 0.000 | 2.366 | -2.366 | 2.366 |
| 497 | LEU | 506 | PHE | 2.340 | 0.000 | 2.340 | 2.340 |
| 88 | THR | 482 | TRP | 3.756 | 1.423 | 2.334 | 2.334 |
| 90 | TYR | 182 | GLU | 0.359 | 2.685 | -2.326 | 2.326 |
| 55 | PHE | 67 | TYR | 2.641 | 4.958 | -2.317 | 2.317 |
| 227 | LEU | 431 | ARG | 0.000 | 2.314 | -2.314 | 2.314 |
| 132 | ILE | 223 | ARG | 4.696 | 7.007 | -2.311 | 2.311 |
| 47 | GLY | 492 | PRO | 1.844 | 4.140 | -2.296 | 2.296 |
| 351 | THR | 352 | GLU | 2.285 | 0.000 | 2.285 | 2.285 |
| 191 | PRO | 466 | THR | 1.381 | 3.660 | -2.279 | 2.279 |
| 414 | SER | 415 | THR | 0.000 | 2.261 | -2.261 | 2.261 |
| 399 | LYS | 400 | VAL | 2.238 | 0.000 | 2.238 | 2.238 |
| 48 | ASN | 492 | PRO | 3.090 | 5.318 | -2.228 | 2.228 |
| 312 | GLU | 428 | LYS | 2.219 | 0.000 | 2.219 | 2.219 |
| 217 | TYR | 441 | ALA | 2.211 | 0.000 | 2.211 | 2.211 |
| 213 | TYR | 445 | SER | 0.000 | 2.180 | -2.180 | 2.180 |
| 221 | GLU | 437 | GLU | 2.170 | 0.000 | 2.170 | 2.170 |
| 393 | THR | 394 | ASN | 2.205 | 4.361 | -2.156 | 2.156 |
| 377 | VAL | 379 | TYR | 0.389 | 2.538 | -2.149 | 2.149 |
| 496 | ALA | 506 | PHE | 0.000 | 2.144 | -2.144 | 2.144 |
| 131 | SER | 139 | ARG | 2.922 | 5.045 | -2.123 | 2.123 |
| 128 | ARG | 444 | LEU | 0.000 | 2.082 | -2.082 | 2.082 |
| 339 | ALA | 340 | GLU | 2.080 | 0.000 | 2.080 | 2.080 |
| 224 | THR | 433 | VAL | 2.046 | 0.000 | 2.046 | 2.046 |
| 421 | ASN | 423 | SER | 3.938 | 1.902 | 2.037 | 2.037 |
| 9 | ALA | 10 | THR | 2.032 | 0.000 | 2.032 | 2.032 |
| 190 | GLU | 191 | PRO | 0.621 | 2.627 | -2.007 | 2.007 |
| 357 | ASP | 358 | THR | 2.573 | 4.572 | -1.999 | 1.999 |
| 461 | MET | 477 | TRP | 3.620 | 5.613 | -1.992 | 1.992 |
| 111 | TYR | 481 | TYR | 1.141 | 3.117 | -1.977 | 1.977 |
| 61 | LEU | 502 | PHE | 6.175 | 4.207 | 1.968 | 1.968 |
| 115 | ASN | 162 | TRP | 4.340 | 6.303 | -1.963 | 1.963 |
| 213 | TYR | 495 | TYR | 1.960 | 0.000 | 1.960 | 1.960 |
| 213 | TYR | 448 | LEU | 3.936 | 5.888 | -1.952 | 1.952 |
| 227 | LEU | 231 | GLN | 0.081 | 2.000 | -1.919 | 1.919 |
| 386 | LYS | 388 | ASP | 2.016 | 3.903 | -1.887 | 1.887 |
| 337 | PHE | 338 | SER | 5.582 | 3.703 | 1.879 | 1.879 |
| 125 | SER | 213 | TYR | 2.046 | 3.913 | -1.867 | 1.867 |
| 347 | VAL | 348 | LYS | 1.862 | 0.000 | 1.862 | 1.862 |
| 96 | TRP | 102 | ALA | 1.514 | 3.371 | -1.857 | 1.857 |
| 286 | GLN | 287 | ALA | 0.512 | 2.367 | -1.855 | 1.855 |
| 390 | ASN | 391 | GLN | 0.037 | 1.865 | -1.828 | 1.828 |
| 227 | LEU | 430 | LYS | 0.000 | 1.819 | -1.819 | 1.819 |
| 195 | PHE | 463 | LEU | 3.158 | 1.342 | 1.816 | 1.816 |
| 481 | TYR | 485 | TYR | 4.655 | 6.446 | -1.792 | 1.792 |
| 106 | TRP | 107 | LEU | 2.267 | 0.492 | 1.776 | 1.776 |
| 222 | LYS | 226 | ASP | 1.880 | 3.655 | -1.775 | 1.775 |
| 84 | MET | 485 | TYR | 4.593 | 6.360 | -1.768 | 1.768 |
| 349 | ALA | 350 | GLU | 2.182 | 0.433 | 1.750 | 1.750 |
| 415 | THR | 416 | LYS | 0.000 | 1.746 | -1.746 | 1.746 |
| 195 | PHE | 199 | ILE | 0.004 | 1.726 | -1.722 | 1.722 |
| 209 | MET | 448 | LEU | 0.337 | 2.048 | -1.711 | 1.711 |
| 427 | THR | 431 | ARG | 1.710 | 0.000 | 1.710 | 1.710 |
| 283 | LYS | 284 | PRO | 1.066 | 2.736 | -1.671 | 1.671 |
| 138 | TYR | 142 | ARG | 1.932 | 0.264 | 1.668 | 1.668 |
| 64 | VAL | 143 | THR | 0.370 | 2.024 | -1.654 | 1.654 |
| 131 | SER | 142 | ARG | 0.502 | 2.150 | -1.648 | 1.648 |
| 124 | ILE | 128 | ARG | 1.790 | 0.149 | 1.641 | 1.641 |
| 67 | TYR | 144 | PRO | 0.535 | 2.174 | -1.638 | 1.638 |
| 190 | GLU | 193 | ILE | 0.748 | 2.384 | -1.636 | 1.636 |
| 511 | LEU | 513 | ARG | 0.000 | 1.623 | -1.623 | 1.623 |
| 115 | ASN | 158 | SER | 8.502 | 6.882 | 1.620 | 1.620 |
| 111 | TYR | 162 | TRP | 10.452 | 8.836 | 1.617 | 1.617 |
| 433 | VAL | 437 | GLU | 0.000 | 1.616 | -1.616 | 1.616 |
| 203 | TYR | 459 | ASN | 2.244 | 3.857 | -1.613 | 1.613 |
| 499 | ASN | 502 | PHE | 1.842 | 3.447 | -1.606 | 1.606 |
| 362 | LEU | 363 | LEU | 0.000 | 1.605 | -1.605 | 1.605 |
| 495 | TYR | 502 | PHE | 0.000 | 1.601 | -1.601 | 1.601 |
| 458 | TYR | 477 | TRP | 1.358 | 2.958 | -1.599 | 1.599 |
| 84 | MET | 482 | TRP | 4.974 | 6.563 | -1.589 | 1.589 |
| 170 | GLN | 186 | GLN | 4.341 | 2.753 | 1.589 | 1.589 |
| 25 | HIS | 30 | VAL | 0.000 | 1.579 | -1.579 | 1.579 |
| 421 | ASN | 422 | PRO | 1.868 | 0.295 | 1.573 | 1.573 |
| 52 | MET | 77 | LEU | 2.270 | 0.700 | 1.570 | 1.570 |
| 12 | VAL | 13 | ASN | 0.000 | 1.558 | -1.558 | 1.558 |
| 370 | ARG | 371 | PRO | 0.193 | 1.746 | -1.553 | 1.553 |
| 353 | LYS | 354 | SER | 1.552 | 0.000 | 1.552 | 1.552 |
| 458 | TYR | 462 | VAL | 3.579 | 2.046 | 1.533 | 1.533 |
| 166 | ILE | 185 | ILE | 0.550 | 2.078 | -1.528 | 1.528 |
| 447 | ILE | 495 | TYR | 3.150 | 1.622 | 1.528 | 1.528 |
| 133 | THR | 223 | ARG | 0.269 | 1.773 | -1.504 | 1.504 |
| 462 | VAL | 477 | TRP | 2.042 | 3.542 | -1.500 | 1.500 |
| 426 | MET | 430 | LYS | 0.000 | 1.493 | -1.493 | 1.493 |
| 368 | ALA | 369 | HIS | 1.476 | 0.000 | 1.476 | 1.476 |
| 454 | THR | 483 | LEU | 8.612 | 10.082 | -1.471 | 1.471 |
| 65 | ASN | 127 | ASP | 1.454 | 0.000 | 1.454 | 1.454 |
| 83 | SER | 485 | TYR | 6.076 | 7.527 | -1.452 | 1.452 |
| 482 | TRP | 485 | TYR | 1.216 | 2.664 | -1.448 | 1.448 |
| 188 | LEU | 466 | THR | 1.444 | 0.000 | 1.444 | 1.444 |
| 134 | ARG | 139 | ARG | 0.189 | 1.619 | -1.430 | 1.430 |
| 455 | TRP | 484 | CYS | 3.409 | 4.838 | -1.429 | 1.429 |
| 135 | PRO | 140 | ALA | 0.000 | 1.427 | -1.427 | 1.427 |
| 101 | LEU | 105 | LEU | 0.146 | 1.565 | -1.419 | 1.419 |
| 346 | PHE | 347 | VAL | 1.418 | 0.000 | 1.418 | 1.418 |
| 51 | VAL | 506 | PHE | 3.356 | 1.939 | 1.418 | 1.418 |
| 55 | PHE | 66 | ASN | 1.877 | 3.262 | -1.385 | 1.385 |
| 87 | TYR | 485 | TYR | 1.850 | 3.231 | -1.381 | 1.381 |
| 170 | GLN | 171 | TYR | 3.541 | 2.165 | 1.376 | 1.376 |
| 90 | TYR | 95 | ARG | 3.616 | 4.977 | -1.361 | 1.361 |
| 221 | GLU | 225 | LYS | 4.909 | 3.553 | 1.356 | 1.356 |
| 187 | PHE | 194 | THR | 1.730 | 0.383 | 1.346 | 1.346 |
| 111 | TYR | 458 | TYR | 2.705 | 1.377 | 1.328 | 1.328 |
| 25 | HIS | 33 | ILE | 0.000 | 1.321 | -1.321 | 1.321 |
| 76 | ASP | 491 | ASN | 5.450 | 4.130 | 1.320 | 1.320 |
| 195 | PHE | 466 | THR | 1.048 | 2.363 | -1.314 | 1.314 |
| 182 | GLU | 184 | GLN | 1.360 | 2.673 | -1.313 | 1.313 |
| 96 | TRP | 98 | LEU | 2.868 | 4.179 | -1.311 | 1.311 |
| 111 | TYR | 185 | ILE | 6.195 | 4.889 | 1.306 | 1.306 |
| 170 | GLN | 176 | ARG | 4.331 | 5.634 | -1.303 | 1.303 |
| 348 | LYS | 349 | ALA | 1.300 | 0.000 | 1.300 | 1.300 |
| 461 | MET | 476 | LEU | 2.749 | 4.048 | -1.299 | 1.299 |
| 45 | ILE | 77 | LEU | 0.962 | 2.259 | -1.298 | 1.298 |
| 449 | LEU | 453 | ILE | 0.170 | 1.464 | -1.294 | 1.294 |
| 278 | THR | 279 | SER | 2.653 | 1.363 | 1.291 | 1.291 |
| 91 | ILE | 482 | TRP | 2.135 | 0.851 | 1.285 | 1.285 |
| 169 | TRP | 187 | PHE | 7.661 | 6.378 | 1.283 | 1.283 |
| 72 | LEU | 495 | TYR | 0.304 | 1.581 | -1.278 | 1.278 |
| 176 | ARG | 186 | GLN | 3.676 | 2.417 | 1.259 | 1.259 |
| 127 | ASP | 138 | TYR | 5.207 | 6.462 | -1.255 | 1.255 |
| 209 | MET | 451 | PHE | 0.000 | 1.254 | -1.254 | 1.254 |
| 341 | GLU | 342 | THR | 1.254 | 0.000 | 1.254 | 1.254 |
| 112 | VAL | 159 | PHE | 1.745 | 2.974 | -1.229 | 1.229 |
| 447 | ILE | 491 | ASN | 0.000 | 1.219 | -1.219 | 1.219 |
| 175 | LYS | 177 | THR | 0.000 | 1.211 | -1.211 | 1.211 |
| 51 | VAL | 495 | TYR | 0.000 | 1.197 | -1.197 | 1.197 |
| 419 | ASN | 420 | PRO | 0.339 | 1.532 | -1.193 | 1.193 |
| 210 | THR | 448 | LEU | 1.190 | 0.000 | 1.190 | 1.190 |
| 66 | ASN | 502 | PHE | 2.109 | 0.935 | 1.175 | 1.175 |
| 116 | ALA | 155 | TRP | 2.192 | 3.353 | -1.160 | 1.160 |
| 71 | SER | 120 | ASN | 6.175 | 5.017 | 1.157 | 1.157 |
| 118 | VAL | 455 | TRP | 0.600 | 1.754 | -1.153 | 1.153 |
| 176 | ARG | 178 | VAL | 0.002 | 1.152 | -1.149 | 1.149 |
| 280 | THR | 281 | THR | 2.749 | 1.615 | 1.134 | 1.134 |
| 463 | LEU | 467 | PHE | 0.000 | 1.116 | -1.116 | 1.116 |
| 1 | MET | 2 | GLU | 0.138 | 1.249 | -1.111 | 1.111 |
| 285 | SER | 286 | GLN | 0.687 | 1.789 | -1.102 | 1.102 |
| 331 | GLU | 332 | SER | 0.453 | 1.552 | -1.099 | 1.099 |
| 96 | TRP | 103 | CYS | 6.552 | 7.649 | -1.097 | 1.097 |
| 231 | GLN | 427 | THR | 0.000 | 1.097 | -1.097 | 1.097 |
| 451 | PHE | 487 | ASN | 3.069 | 4.151 | -1.082 | 1.082 |
| 41 | SER | 84 | MET | 2.686 | 1.607 | 1.079 | 1.079 |
| 122 | LEU | 209 | MET | 2.026 | 3.081 | -1.055 | 1.055 |
| 494 | CYS | 495 | TYR | 1.055 | 0.000 | 1.055 | 1.055 |
| 162 | TRP | 197 | THR | 2.881 | 1.835 | 1.046 | 1.046 |
| 87 | TYR | 91 | ILE | 2.041 | 0.998 | 1.043 | 1.043 |
| 256 | CYS | 257 | PRO | 0.482 | 1.521 | -1.040 | 1.040 |
| 125 | SER | 209 | MET | 1.608 | 2.643 | -1.034 | 1.034 |
| 71 | SER | 151 | ILE | 1.963 | 2.994 | -1.031 | 1.031 |
| 129 | TYR | 133 | THR | 3.555 | 2.533 | 1.022 | 1.022 |
| 269 | ALA | 270 | SER | 1.022 | 0.000 | 1.022 | 1.022 |
| 493 | ILE | 498 | CYS | 1.020 | 0.000 | 1.020 | 1.020 |
| 300 | LEU | 302 | THR | 0.000 | 1.018 | -1.018 | 1.018 |
| 124 | ILE | 444 | LEU | 0.000 | 1.010 | -1.010 | 1.010 |
| 72 | LEU | 491 | ASN | 1.597 | 2.605 | -1.008 | 1.008 |
| 20 | GLN | 21 | PRO | 1.352 | 0.351 | 1.001 | 1.001 |
| 354 | SER | 356 | TYR | 0.000 | 1.001 | -1.001 | 1.001 |
| 124 | ILE | 495 | TYR | 0.999 | 0.000 | 0.999 | 0.999 |
| 249 | LEU | 250 | PHE | 0.000 | 0.994 | -0.994 | 0.994 |
| 24 | ARG | 28 | TRP | 0.991 | 0.000 | 0.991 | 0.991 |
| 81 | ILE | 82 | PHE | 3.083 | 4.071 | -0.988 | 0.988 |
| 64 | VAL | 144 | PRO | 1.031 | 2.018 | -0.987 | 0.987 |
| 453 | ILE | 483 | LEU | 1.617 | 0.632 | 0.985 | 0.985 |
| 382 | ARG | 402 | ILE | 0.000 | 0.976 | -0.976 | 0.976 |
| 220 | THR | 438 | ARG | 0.000 | 0.962 | -0.962 | 0.962 |
| 202 | PHE | 451 | PHE | 1.822 | 0.868 | 0.954 | 0.954 |
| 500 | ARG | 504 | LYS | 0.952 | 0.000 | 0.952 | 0.952 |
| 115 | ASN | 161 | LEU | 0.950 | 0.000 | 0.950 | 0.950 |
| 17 | VAL | 19 | HIS | 0.000 | 0.941 | -0.941 | 0.941 |
| 68 | TYR | 127 | ASP | 5.669 | 6.611 | -0.941 | 0.941 |
| 40 | VAL | 84 | MET | 1.822 | 0.883 | 0.939 | 0.939 |
| 106 | TRP | 110 | ASP | 0.338 | 1.276 | -0.938 | 0.938 |
| 157 | ILE | 161 | LEU | 0.000 | 0.937 | -0.937 | 0.937 |
| 118 | VAL | 201 | ALA | 1.373 | 0.437 | 0.937 | 0.937 |
| 56 | LYS | 62 | LYS | 2.372 | 3.303 | -0.931 | 0.931 |
| 323 | VAL | 325 | TYR | 0.000 | 0.930 | -0.930 | 0.930 |
| 173 | VAL | 175 | LYS | 0.000 | 0.929 | -0.929 | 0.929 |
| 292 | ALA | 293 | ASN | 0.000 | 0.928 | -0.928 | 0.928 |
| 178 | VAL | 184 | GLN | 3.452 | 2.528 | 0.924 | 0.924 |
| 79 | ILE | 113 | ALA | 1.885 | 0.961 | 0.923 | 0.923 |
| 217 | TYR | 221 | GLU | 0.395 | 1.317 | -0.922 | 0.922 |
| 185 | ILE | 187 | PHE | 4.980 | 4.058 | 0.922 | 0.922 |
| 55 | PHE | 70 | LEU | 1.239 | 2.161 | -0.922 | 0.922 |
| 162 | TRP | 201 | ALA | 1.346 | 0.439 | 0.907 | 0.907 |
| 338 | SER | 339 | ALA | 0.904 | 0.000 | 0.904 | 0.904 |
| 524 | TYR | 528 | ASN | 0.000 | 0.891 | -0.891 | 0.891 |
| 23 | GLU | 475 | THR | 0.000 | 0.889 | -0.889 | 0.889 |
| 104 | ASP | 186 | GLN | 0.883 | 0.000 | 0.883 | 0.883 |
| 508 | MET | 512 | CYS | 0.881 | 0.000 | 0.881 | 0.881 |
| 37 | THR | 482 | TRP | 5.141 | 6.015 | -0.874 | 0.874 |
| 117 | SER | 491 | ASN | 0.898 | 0.026 | 0.872 | 0.872 |
| 90 | TYR | 96 | TRP | 3.581 | 4.450 | -0.869 | 0.869 |
| 251 | ARG | 252 | SER | 0.864 | 0.000 | 0.864 | 0.864 |
| 106 | TRP | 481 | TYR | 0.863 | 0.000 | 0.863 | 0.863 |
| 44 | THR | 84 | MET | 2.134 | 2.996 | -0.863 | 0.863 |
| 34 | ALA | 92 | LEU | 2.244 | 1.383 | 0.861 | 0.861 |
| 165 | ALA | 187 | PHE | 3.017 | 3.876 | -0.859 | 0.859 |
| 68 | TYR | 147 | ALA | 4.134 | 4.985 | -0.851 | 0.851 |
| 129 | TYR | 212 | LEU | 3.675 | 2.826 | 0.849 | 0.849 |
| 68 | TYR | 124 | ILE | 1.784 | 2.628 | -0.844 | 0.844 |
| 83 | SER | 106 | TRP | 2.556 | 1.715 | 0.841 | 0.841 |
| 185 | ILE | 188 | LEU | 1.824 | 2.664 | -0.840 | 0.840 |
| 72 | LEU | 117 | SER | 2.815 | 3.645 | -0.830 | 0.830 |
| 335 | GLU | 336 | GLU | 0.828 | 0.000 | 0.828 | 0.828 |
| 505 | THR | 514 | TRP | 0.000 | 0.825 | -0.825 | 0.825 |
| 141 | LYS | 146 | ARG | 4.385 | 3.560 | 0.825 | 0.825 |
| 287 | ALA | 288 | THR | 1.029 | 0.210 | 0.819 | 0.819 |
| 262 | ALA | 263 | GLN | 0.000 | 0.816 | -0.816 | 0.816 |
| 33 | ILE | 92 | LEU | 0.331 | 1.145 | -0.814 | 0.814 |
| 194 | THR | 466 | THR | 2.099 | 1.287 | 0.812 | 0.812 |
| 47 | GLY | 506 | PHE | 0.811 | 0.000 | 0.811 | 0.811 |
| 203 | TYR | 463 | LEU | 2.491 | 1.689 | 0.802 | 0.802 |
| 458 | TYR | 459 | ASN | 0.186 | 0.984 | -0.797 | 0.797 |
| 492 | PRO | 506 | PHE | 0.000 | 0.797 | -0.797 | 0.797 |
| 128 | ARG | 447 | ILE | 0.793 | 0.000 | 0.793 | 0.793 |
| 178 | VAL | 186 | GLN | 0.780 | 1.568 | -0.789 | 0.789 |
| 76 | ASP | 117 | SER | 2.749 | 1.962 | 0.787 | 0.787 |
| 112 | VAL | 158 | SER | 3.894 | 3.131 | 0.763 | 0.763 |
| 217 | TYR | 444 | LEU | 0.759 | 0.000 | 0.759 | 0.759 |
| 199 | ILE | 203 | TYR | 0.218 | 0.971 | -0.753 | 0.753 |
| 135 | PRO | 223 | ARG | 0.824 | 0.085 | 0.740 | 0.740 |
| 120 | ASN | 151 | ILE | 0.000 | 0.736 | -0.736 | 0.736 |
| 517 | LYS | 520 | GLU | 0.000 | 0.736 | -0.736 | 0.736 |
| 279 | SER | 280 | THR | 1.564 | 2.296 | -0.733 | 0.733 |
| 447 | ILE | 494 | CYS | 2.585 | 1.856 | 0.729 | 0.729 |
| 206 | VAL | 451 | PHE | 1.406 | 0.679 | 0.727 | 0.727 |
| 108 | ALA | 167 | LEU | 1.426 | 0.705 | 0.721 | 0.721 |
| 44 | THR | 489 | THR | 5.795 | 6.514 | -0.719 | 0.719 |
| 96 | TRP | 181 | ASP | 4.051 | 3.335 | 0.717 | 0.717 |
| 531 | LEU | 532 | PRO | 1.397 | 0.680 | 0.717 | 0.717 |
| 122 | LEU | 205 | PRO | 1.270 | 0.555 | 0.715 | 0.715 |
| 260 | THR | 261 | LEU | 0.000 | 0.711 | -0.711 | 0.711 |
| 125 | SER | 212 | LEU | 2.553 | 1.847 | 0.707 | 0.707 |
| 461 | MET | 472 | VAL | 1.356 | 2.063 | -0.706 | 0.706 |
| 121 | LEU | 447 | ILE | 0.000 | 0.702 | -0.702 | 0.702 |
| 209 | MET | 213 | TYR | 0.701 | 0.000 | 0.701 | 0.701 |
| 104 | ASP | 176 | ARG | 6.515 | 7.214 | -0.699 | 0.699 |
| 498 | CYS | 503 | ARG | 0.694 | 0.000 | 0.694 | 0.694 |
| 91 | ILE | 478 | HIS | 2.198 | 2.890 | -0.693 | 0.693 |
| 458 | TYR | 484 | CYS | 2.156 | 1.464 | 0.692 | 0.692 |
| 184 | GLN | 189 | SER | 0.000 | 0.692 | -0.692 | 0.692 |
| 121 | LEU | 451 | PHE | 1.525 | 2.216 | -0.691 | 0.691 |
| 51 | VAL | 496 | ALA | 0.683 | 0.000 | 0.683 | 0.683 |
| 72 | LEU | 121 | LEU | 2.127 | 1.445 | 0.682 | 0.682 |
| 68 | TYR | 142 | ARG | 0.681 | 0.000 | 0.681 | 0.681 |
| 58 | ASN | 60 | GLN | 2.063 | 2.741 | -0.678 | 0.678 |
| 107 | LEU | 183 | CYS | 1.180 | 1.857 | -0.677 | 0.677 |
| 61 | LEU | 66 | ASN | 5.241 | 5.912 | -0.672 | 0.672 |
| 86 | LEU | 106 | TRP | 2.861 | 3.526 | -0.665 | 0.665 |
| 216 | ILE | 444 | LEU | 0.664 | 0.000 | 0.664 | 0.664 |
| 405 | CYS | 406 | PRO | 1.192 | 0.538 | 0.653 | 0.653 |
| 233 | SER | 234 | ASP | 0.639 | 0.000 | 0.639 | 0.639 |
| 308 | SER | 310 | GLU | 0.098 | 0.734 | -0.636 | 0.636 |
| 41 | SER | 85 | ASN | 3.567 | 4.199 | -0.632 | 0.632 |
| 454 | THR | 484 | CYS | 2.754 | 2.127 | 0.626 | 0.626 |
| 84 | MET | 489 | THR | 0.091 | 0.710 | -0.619 | 0.619 |
| 454 | THR | 487 | ASN | 4.832 | 4.218 | 0.614 | 0.614 |
| 4 | ASP | 5 | SER | 0.608 | 0.000 | 0.608 | 0.608 |
| 75 | ALA | 155 | TRP | 2.631 | 3.236 | -0.604 | 0.604 |
| 90 | TYR | 478 | HIS | 0.603 | 0.000 | 0.603 | 0.603 |
| 87 | TYR | 90 | TYR | 1.157 | 0.555 | 0.602 | 0.602 |
| 502 | PHE | 506 | PHE | 0.526 | 1.127 | -0.602 | 0.602 |
| 63 | THR | 65 | ASN | 0.652 | 1.252 | -0.599 | 0.599 |
| 454 | THR | 486 | VAL | 0.790 | 0.191 | 0.598 | 0.598 |
| 119 | MET | 154 | ALA | 2.715 | 2.117 | 0.598 | 0.598 |
| 188 | LEU | 462 | VAL | 0.596 | 0.000 | 0.596 | 0.596 |
| 107 | LEU | 185 | ILE | 1.109 | 1.703 | -0.593 | 0.593 |
| 75 | ALA | 116 | ALA | 0.592 | 0.000 | 0.592 | 0.592 |
| 110 | ASP | 481 | TYR | 2.976 | 3.566 | -0.590 | 0.590 |
| 118 | VAL | 205 | PRO | 1.130 | 0.547 | 0.583 | 0.583 |
| 111 | TYR | 188 | LEU | 0.000 | 0.578 | -0.578 | 0.578 |
| 123 | VAL | 150 | MET | 1.640 | 1.064 | 0.576 | 0.576 |
| 206 | VAL | 452 | ILE | 0.049 | 0.621 | -0.573 | 0.573 |
| 119 | MET | 200 | ALA | 0.572 | 0.000 | 0.572 | 0.572 |
| 473 | PRO | 475 | THR | 0.563 | 0.000 | 0.563 | 0.563 |
| 468 | CYS | 471 | CYS | 3.736 | 3.175 | 0.562 | 0.562 |
| 4 | ASP | 6 | TYR | 0.000 | 0.561 | -0.561 | 0.561 |
| 161 | LEU | 201 | ALA | 0.561 | 0.000 | 0.561 | 0.561 |
| 203 | TYR | 460 | ILE | 0.997 | 1.556 | -0.559 | 0.559 |
| 139 | ARG | 433 | VAL | 0.000 | 0.558 | -0.558 | 0.558 |
| 130 | PHE | 134 | ARG | 0.647 | 1.205 | -0.558 | 0.558 |
| 128 | ARG | 213 | TYR | 0.816 | 0.260 | 0.556 | 0.556 |
| 121 | LEU | 491 | ASN | 0.554 | 0.000 | 0.554 | 0.554 |
| 227 | LEU | 433 | VAL | 0.543 | 0.000 | 0.543 | 0.543 |
| 48 | ASN | 73 | ALA | 4.904 | 5.446 | -0.542 | 0.542 |
| 497 | LEU | 503 | ARG | 4.038 | 3.500 | 0.539 | 0.539 |
| 55 | PHE | 62 | LYS | 4.105 | 4.643 | -0.538 | 0.538 |
| 169 | TRP | 186 | GLN | 3.223 | 3.761 | -0.538 | 0.538 |
| 40 | VAL | 482 | TRP | 0.000 | 0.533 | -0.533 | 0.533 |
| 64 | VAL | 67 | TYR | 0.531 | 0.000 | 0.531 | 0.531 |
| 234 | ASP | 423 | SER | 0.000 | 0.529 | -0.529 | 0.529 |
| 65 | ASN | 66 | ASN | 0.450 | 0.976 | -0.527 | 0.527 |
| 28 | TRP | 32 | THR | 0.000 | 0.524 | -0.524 | 0.524 |
| 198 | ALA | 462 | VAL | 0.000 | 0.519 | -0.519 | 0.519 |
| 61 | LEU | 505 | THR | 0.775 | 1.289 | -0.514 | 0.514 |
| 53 | ILE | 509 | LEU | 1.068 | 1.579 | -0.511 | 0.511 |
| 466 | THR | 467 | PHE | 2.431 | 1.926 | 0.505 | 0.505 |
| 213 | TYR | 447 | ILE | 0.503 | 0.000 | 0.503 | 0.503 |
| 450 | ALA | 490 | VAL | 0.172 | 0.672 | -0.500 | 0.500 |
| 126 | PHE | 130 | PHE | 1.143 | 1.641 | -0.497 | 0.497 |
| 106 | TRP | 485 | TYR | 0.418 | 0.913 | -0.495 | 0.495 |
| 199 | ILE | 204 | ILE | 6.187 | 5.692 | 0.494 | 0.494 |
| 499 | ASN | 501 | THR | 1.878 | 1.386 | 0.491 | 0.491 |
| 122 | LEU | 125 | SER | 0.487 | 0.000 | 0.487 | 0.487 |
| 138 | TYR | 150 | MET | 0.000 | 0.487 | -0.487 | 0.487 |
| 55 | PHE | 61 | LEU | 3.446 | 2.961 | 0.485 | 0.485 |
| 169 | TRP | 190 | GLU | 0.477 | 0.000 | 0.477 | 0.477 |
| 72 | LEU | 124 | ILE | 0.470 | 0.000 | 0.470 | 0.470 |
| 18 | ASN | 20 | GLN | 0.469 | 0.000 | 0.469 | 0.469 |
| 318 | ASP | 319 | PRO | 0.314 | 0.779 | -0.465 | 0.465 |
| 209 | MET | 495 | TYR | 0.462 | 0.000 | 0.462 | 0.462 |
| 48 | ASN | 488 | SER | 1.523 | 1.982 | -0.459 | 0.459 |
| 490 | VAL | 494 | CYS | 0.214 | 0.667 | -0.453 | 0.453 |
| 37 | THR | 88 | THR | 3.991 | 4.442 | -0.451 | 0.451 |
| 524 | TYR | 525 | TRP | 0.000 | 0.450 | -0.450 | 0.450 |
| 210 | THR | 452 | ILE | 0.000 | 0.449 | -0.449 | 0.449 |
| 48 | ASN | 76 | ASP | 4.616 | 4.173 | 0.443 | 0.443 |
| 151 | ILE | 155 | TRP | 0.474 | 0.915 | -0.440 | 0.440 |
| 68 | TYR | 123 | VAL | 2.487 | 2.048 | 0.438 | 0.438 |
| 96 | TRP | 182 | GLU | 0.562 | 1.001 | -0.438 | 0.438 |
| 122 | LEU | 126 | PHE | 2.007 | 1.572 | 0.435 | 0.435 |
| 103 | CYS | 183 | CYS | 6.346 | 6.779 | -0.433 | 0.433 |
| 58 | ASN | 505 | THR | 3.036 | 2.605 | 0.431 | 0.431 |
| 54 | SER | 506 | PHE | 3.100 | 2.676 | 0.424 | 0.424 |
| 464 | VAL | 471 | CYS | 0.000 | 0.422 | -0.422 | 0.422 |
| 452 | ILE | 456 | THR | 1.220 | 0.799 | 0.420 | 0.420 |
| 30 | VAL | 92 | LEU | 1.990 | 2.409 | -0.419 | 0.419 |
| 296 | LYS | 298 | GLU | 0.000 | 0.416 | -0.416 | 0.416 |
| 309 | SER | 311 | ASP | 0.415 | 0.000 | 0.415 | 0.415 |
| 87 | TYR | 477 | TRP | 0.212 | 0.627 | -0.414 | 0.414 |
| 491 | ASN | 496 | ALA | 0.413 | 0.000 | 0.413 | 0.413 |
| 161 | LEU | 200 | ALA | 0.413 | 0.000 | 0.413 | 0.413 |
| 96 | TRP | 183 | CYS | 3.447 | 3.040 | 0.407 | 0.407 |
| 15 | THR | 16 | PRO | 0.942 | 0.536 | 0.406 | 0.406 |
| 33 | ILE | 91 | ILE | 0.000 | 0.406 | -0.406 | 0.406 |
| 284 | PRO | 285 | SER | 0.000 | 0.405 | -0.405 | 0.405 |
| 343 | GLU | 344 | GLU | 0.404 | 0.000 | 0.404 | 0.404 |
| 132 | ILE | 216 | ILE | 1.340 | 0.938 | 0.402 | 0.402 |
| 465 | SER | 477 | TRP | 1.870 | 1.470 | 0.400 | 0.400 |
| 258 | ARG | 260 | THR | 0.000 | 0.396 | -0.396 | 0.396 |
| 58 | ASN | 501 | THR | 1.127 | 0.732 | 0.395 | 0.395 |
| 177 | THR | 178 | VAL | 0.393 | 0.000 | 0.393 | 0.393 |
| 188 | LEU | 197 | THR | 0.000 | 0.390 | -0.390 | 0.390 |
| 394 | ASN | 395 | ASN | 0.599 | 0.981 | -0.383 | 0.383 |
| 52 | MET | 74 | CYS | 0.252 | 0.634 | -0.382 | 0.382 |
| 223 | ARG | 437 | GLU | 0.000 | 0.381 | -0.381 | 0.381 |
| 103 | CYS | 104 | ASP | 0.471 | 0.851 | -0.379 | 0.379 |
| 44 | THR | 488 | SER | 1.720 | 2.097 | -0.377 | 0.377 |
| 5 | SER | 7 | HIS | 0.286 | 0.663 | -0.377 | 0.377 |
| 457 | PRO | 483 | LEU | 1.077 | 1.444 | -0.368 | 0.368 |
| 60 | GLN | 501 | THR | 0.330 | 0.697 | -0.367 | 0.367 |
| 129 | TYR | 216 | ILE | 3.209 | 2.843 | 0.366 | 0.366 |
| 450 | ALA | 494 | CYS | 0.364 | 0.000 | 0.364 | 0.364 |
| 79 | ILE | 114 | SER | 1.817 | 2.180 | -0.363 | 0.363 |
| 106 | TRP | 183 | CYS | 1.975 | 2.337 | -0.361 | 0.361 |
| 45 | ILE | 81 | ILE | 1.677 | 2.031 | -0.354 | 0.354 |
| 143 | THR | 146 | ARG | 1.765 | 2.118 | -0.353 | 0.353 |
| 125 | SER | 448 | LEU | 0.000 | 0.352 | -0.352 | 0.352 |
| 252 | SER | 253 | CYS | 0.483 | 0.833 | -0.349 | 0.349 |
| 112 | VAL | 162 | TRP | 0.659 | 1.006 | -0.347 | 0.347 |
| 57 | VAL | 505 | THR | 0.488 | 0.141 | 0.347 | 0.347 |
| 276 | ARG | 277 | SER | 0.545 | 0.891 | -0.346 | 0.346 |
| 198 | ALA | 203 | TYR | 4.357 | 4.015 | 0.342 | 0.342 |
| 444 | LEU | 447 | ILE | 0.000 | 0.342 | -0.342 | 0.342 |
| 49 | VAL | 52 | MET | 0.341 | 0.000 | 0.341 | 0.341 |
| 216 | ILE | 441 | ALA | 0.000 | 0.339 | -0.339 | 0.339 |
| 108 | ALA | 166 | ILE | 2.244 | 2.582 | -0.338 | 0.338 |
| 51 | VAL | 492 | PRO | 0.779 | 0.441 | 0.338 | 0.338 |
| 392 | GLU | 393 | THR | 1.436 | 1.099 | 0.337 | 0.337 |
| 314 | LYS | 315 | PRO | 1.245 | 0.908 | 0.337 | 0.337 |
| 61 | LEU | 501 | THR | 2.426 | 2.089 | 0.337 | 0.337 |
| 124 | ILE | 213 | TYR | 0.000 | 0.337 | -0.337 | 0.337 |
| 119 | MET | 157 | ILE | 0.336 | 0.000 | 0.336 | 0.336 |
| 66 | ASN | 69 | LEU | 0.720 | 1.056 | -0.336 | 0.336 |
| 79 | ILE | 84 | MET | 4.691 | 4.355 | 0.335 | 0.335 |
| 79 | ILE | 83 | SER | 1.004 | 1.333 | -0.330 | 0.330 |
| 127 | ASP | 142 | ARG | 6.080 | 6.408 | -0.328 | 0.328 |
| 78 | ILE | 155 | TRP | 0.807 | 0.481 | 0.326 | 0.326 |
| 463 | LEU | 466 | THR | 0.000 | 0.322 | -0.322 | 0.322 |
| 461 | MET | 480 | GLY | 1.249 | 0.928 | 0.321 | 0.321 |
| 119 | MET | 205 | PRO | 0.320 | 0.000 | 0.320 | 0.320 |
| 127 | ASP | 131 | SER | 0.477 | 0.158 | 0.319 | 0.319 |
| 108 | ALA | 163 | ALA | 0.168 | 0.486 | -0.318 | 0.318 |
| 454 | THR | 455 | TRP | 1.773 | 1.456 | 0.316 | 0.316 |
| 452 | ILE | 457 | PRO | 0.316 | 0.000 | 0.316 | 0.316 |
| 509 | LEU | 514 | TRP | 0.000 | 0.314 | -0.314 | 0.314 |
| 491 | ASN | 495 | TYR | 0.793 | 0.480 | 0.312 | 0.312 |
| 169 | TRP | 193 | ILE | 1.480 | 1.169 | 0.311 | 0.311 |
| 75 | ALA | 117 | SER | 1.168 | 0.860 | 0.308 | 0.308 |
| 344 | GLU | 345 | THR | 0.307 | 0.000 | 0.307 | 0.307 |
| 68 | TYR | 151 | ILE | 2.606 | 2.910 | -0.303 | 0.303 |
| 55 | PHE | 63 | THR | 0.489 | 0.186 | 0.303 | 0.303 |
| 361 | TYR | 362 | LEU | 0.000 | 0.303 | -0.303 | 0.303 |
| 169 | TRP | 173 | VAL | 3.056 | 2.753 | 0.303 | 0.303 |
| 132 | ILE | 220 | THR | 0.000 | 0.301 | -0.301 | 0.301 |
| 133 | THR | 134 | ARG | 0.805 | 1.105 | -0.300 | 0.300 |
| 443 | THR | 447 | ILE | 0.582 | 0.282 | 0.300 | 0.300 |
| 110 | ASP | 485 | TYR | 6.840 | 6.545 | 0.295 | 0.295 |
| 178 | VAL | 182 | GLU | 0.492 | 0.197 | 0.295 | 0.295 |
| 367 | ALA | 368 | ALA | 0.295 | 0.000 | 0.295 | 0.295 |
| 423 | SER | 424 | HIS | 0.292 | 0.000 | 0.292 | 0.292 |
| 508 | MET | 509 | LEU | 0.288 | 0.000 | 0.288 | 0.288 |
| 217 | TYR | 437 | GLU | 0.287 | 0.000 | 0.287 | 0.287 |
| 321 | LEU | 323 | VAL | 0.623 | 0.909 | -0.286 | 0.286 |
| 200 | ALA | 205 | PRO | 1.829 | 2.115 | -0.286 | 0.286 |
| 198 | ALA | 463 | LEU | 0.285 | 0.000 | 0.285 | 0.285 |
| 121 | LEU | 487 | ASN | 0.284 | 0.000 | 0.284 | 0.284 |
| 122 | LEU | 208 | VAL | 1.259 | 0.979 | 0.280 | 0.280 |
| 400 | VAL | 402 | ILE | 0.406 | 0.127 | 0.280 | 0.280 |
| 90 | TYR | 181 | ASP | 0.273 | 0.547 | -0.274 | 0.274 |
| 193 | ILE | 194 | THR | 0.274 | 0.000 | 0.274 | 0.274 |
| 62 | LYS | 67 | TYR | 0.000 | 0.271 | -0.271 | 0.271 |
| 464 | VAL | 468 | CYS | 0.000 | 0.270 | -0.270 | 0.270 |
| 243 | LYS | 244 | PRO | 0.000 | 0.266 | -0.266 | 0.266 |
| 76 | ASP | 79 | ILE | 0.266 | 0.000 | 0.266 | 0.266 |
| 87 | TYR | 478 | HIS | 6.117 | 5.852 | 0.265 | 0.265 |
| 487 | ASN | 490 | VAL | 0.263 | 0.000 | 0.263 | 0.263 |
| 178 | VAL | 179 | PRO | 1.275 | 1.012 | 0.263 | 0.263 |
| 8 | ASN | 9 | ALA | 0.263 | 0.000 | 0.263 | 0.263 |
| 54 | SER | 61 | LEU | 2.223 | 2.484 | -0.261 | 0.261 |
| 120 | ASN | 154 | ALA | 0.264 | 0.525 | -0.260 | 0.260 |
| 369 | HIS | 370 | ARG | 0.259 | 0.000 | 0.259 | 0.259 |
| 345 | THR | 347 | VAL | 0.254 | 0.000 | 0.254 | 0.254 |
| 208 | VAL | 212 | LEU | 0.970 | 0.716 | 0.254 | 0.254 |
| 54 | SER | 505 | THR | 3.090 | 3.343 | -0.253 | 0.253 |
| 275 | ARG | 276 | ARG | 0.252 | 0.000 | 0.252 | 0.252 |
| 223 | ARG | 226 | ASP | 0.000 | 0.251 | -0.251 | 0.251 |
| 91 | ILE | 92 | LEU | 0.000 | 0.249 | -0.249 | 0.249 |
| 245 | ALA | 246 | HIS | 0.247 | 0.000 | 0.247 | 0.247 |
| 211 | ILE | 215 | ARG | 0.932 | 1.177 | -0.245 | 0.245 |
| 143 | THR | 144 | PRO | 1.169 | 0.924 | 0.245 | 0.245 |
| 326 | LYS | 327 | SER | 0.244 | 0.000 | 0.244 | 0.244 |
| 41 | SER | 80 | GLY | 1.532 | 1.776 | -0.243 | 0.243 |
| 306 | TYR | 307 | PRO | 0.990 | 0.747 | 0.243 | 0.243 |
| 383 | LEU | 385 | VAL | 0.000 | 0.243 | -0.243 | 0.243 |
| 365 | PRO | 366 | ALA | 0.497 | 0.255 | 0.242 | 0.242 |
| 113 | ALA | 155 | TRP | 0.000 | 0.242 | -0.242 | 0.242 |
| 494 | CYS | 499 | ASN | 0.000 | 0.241 | -0.241 | 0.241 |
| 44 | THR | 76 | ASP | 0.702 | 0.942 | -0.240 | 0.240 |
| 52 | MET | 73 | ALA | 0.518 | 0.279 | 0.239 | 0.239 |
| 128 | ARG | 216 | ILE | 1.054 | 1.292 | -0.238 | 0.238 |
| 235 | SER | 236 | VAL | 0.421 | 0.183 | 0.237 | 0.237 |
| 69 | LEU | 502 | PHE | 0.697 | 0.460 | 0.237 | 0.237 |
| 50 | LEU | 509 | LEU | 0.677 | 0.914 | -0.237 | 0.237 |
| 84 | MET | 85 | ASN | 0.040 | 0.277 | -0.237 | 0.237 |
| 105 | LEU | 167 | LEU | 1.186 | 0.950 | 0.236 | 0.236 |
| 202 | PHE | 455 | TRP | 3.586 | 3.818 | -0.232 | 0.232 |
| 107 | LEU | 166 | ILE | 1.879 | 2.109 | -0.230 | 0.230 |
| 234 | ASP | 424 | HIS | 0.000 | 0.230 | -0.230 | 0.230 |
| 403 | MET | 404 | PRO | 1.186 | 0.957 | 0.229 | 0.229 |
| 446 | ALA | 494 | CYS | 0.000 | 0.227 | -0.227 | 0.227 |
| 168 | CYS | 172 | LEU | 0.224 | 0.000 | 0.224 | 0.224 |
| 505 | THR | 508 | MET | 0.223 | 0.000 | 0.223 | 0.223 |
| 112 | VAL | 163 | ALA | 1.001 | 1.223 | -0.222 | 0.222 |
| 189 | SER | 194 | THR | 0.000 | 0.220 | -0.220 | 0.220 |
| 40 | VAL | 489 | THR | 1.690 | 1.910 | -0.219 | 0.219 |
| 166 | ILE | 187 | PHE | 1.287 | 1.505 | -0.218 | 0.218 |
| 79 | ILE | 110 | ASP | 0.068 | 0.285 | -0.218 | 0.218 |
| 68 | TYR | 120 | ASN | 0.590 | 0.376 | 0.214 | 0.214 |
| 84 | MET | 488 | SER | 0.000 | 0.211 | -0.211 | 0.211 |
| 63 | THR | 66 | ASN | 3.218 | 3.008 | 0.210 | 0.210 |
| 104 | ASP | 166 | ILE | 0.932 | 1.142 | -0.209 | 0.209 |
| 202 | PHE | 203 | TYR | 3.492 | 3.284 | 0.208 | 0.208 |
| 133 | THR | 219 | GLU | 0.000 | 0.206 | -0.206 | 0.206 |
| 53 | ILE | 57 | VAL | 0.581 | 0.376 | 0.205 | 0.205 |
| 497 | LEU | 498 | CYS | 0.000 | 0.203 | -0.203 | 0.203 |
| 413 | PRO | 414 | SER | 0.000 | 0.203 | -0.203 | 0.203 |
| 52 | MET | 70 | LEU | 1.422 | 1.625 | -0.203 | 0.203 |
| 446 | ALA | 498 | CYS | 0.000 | 0.202 | -0.202 | 0.202 |
| 121 | LEU | 448 | LEU | 0.000 | 0.201 | -0.201 | 0.201 |
| 129 | TYR | 130 | PHE | 2.464 | 2.263 | 0.201 | 0.201 |
| 486 | VAL | 490 | VAL | 0.166 | 0.367 | -0.201 | 0.201 |
| 450 | ALA | 487 | ASN | 0.372 | 0.571 | -0.200 | 0.200 |
| 84 | MET | 486 | VAL | 0.000 | 0.200 | -0.200 | 0.200 |
| 187 | PHE | 193 | ILE | 2.661 | 2.462 | 0.199 | 0.199 |
| 159 | PHE | 164 | PRO | 1.784 | 1.975 | -0.191 | 0.191 |
| 75 | ALA | 76 | ASP | 0.000 | 0.189 | -0.189 | 0.189 |
| 115 | ASN | 157 | ILE | 0.187 | 0.000 | 0.187 | 0.187 |
| 110 | ASP | 114 | SER | 0.825 | 1.011 | -0.186 | 0.186 |
| 54 | SER | 509 | LEU | 1.693 | 1.879 | -0.186 | 0.186 |
| 49 | VAL | 77 | LEU | 0.649 | 0.833 | -0.185 | 0.185 |
| 40 | VAL | 44 | THR | 0.067 | 0.249 | -0.182 | 0.182 |
| 191 | PRO | 195 | PHE | 0.181 | 0.000 | 0.181 | 0.181 |
| 114 | SER | 455 | TRP | 2.409 | 2.589 | -0.180 | 0.180 |
| 231 | GLN | 430 | LYS | 0.180 | 0.000 | 0.180 | 0.180 |
| 220 | THR | 440 | ALA | 0.176 | 0.000 | 0.176 | 0.176 |
| 455 | TRP | 459 | ASN | 0.912 | 0.737 | 0.175 | 0.175 |
| 58 | ASN | 61 | LEU | 2.525 | 2.694 | -0.169 | 0.169 |
| 189 | SER | 190 | GLU | 0.166 | 0.000 | 0.166 | 0.166 |
| 213 | TYR | 441 | ALA | 0.000 | 0.164 | -0.164 | 0.164 |
| 451 | PHE | 452 | ILE | 0.000 | 0.162 | -0.162 | 0.162 |
| 197 | THR | 202 | PHE | 0.000 | 0.161 | -0.161 | 0.161 |
| 125 | SER | 126 | PHE | 0.161 | 0.000 | 0.161 | 0.161 |
| 51 | VAL | 73 | ALA | 0.821 | 0.981 | -0.160 | 0.160 |
| 126 | PHE | 208 | VAL | 1.413 | 1.255 | 0.158 | 0.158 |
| 119 | MET | 158 | SER | 0.900 | 1.058 | -0.158 | 0.158 |
| 519 | VAL | 523 | LEU | 0.652 | 0.809 | -0.157 | 0.157 |
| 372 | LYS | 373 | SER | 0.155 | 0.000 | 0.155 | 0.155 |
| 45 | ILE | 49 | VAL | 0.289 | 0.135 | 0.154 | 0.154 |
| 212 | LEU | 213 | TYR | 0.000 | 0.154 | -0.154 | 0.154 |
| 82 | PHE | 106 | TRP | 0.154 | 0.000 | 0.154 | 0.154 |
| 135 | PRO | 139 | ARG | 0.000 | 0.153 | -0.153 | 0.153 |
| 156 | LEU | 160 | ILE | 1.621 | 1.469 | 0.152 | 0.152 |
| 358 | THR | 360 | ASN | 0.000 | 0.152 | -0.152 | 0.152 |
| 61 | LEU | 499 | ASN | 0.152 | 0.000 | 0.152 | 0.152 |
| 64 | VAL | 147 | ALA | 0.828 | 0.978 | -0.150 | 0.150 |
| 72 | LEU | 76 | ASP | 1.819 | 1.969 | -0.150 | 0.150 |
| 96 | TRP | 180 | LEU | 0.514 | 0.664 | -0.150 | 0.150 |
| 493 | ILE | 497 | LEU | 0.686 | 0.834 | -0.149 | 0.149 |
| 40 | VAL | 486 | VAL | 0.000 | 0.148 | -0.148 | 0.148 |
| 304 | SER | 306 | TYR | 0.000 | 0.147 | -0.147 | 0.147 |
| 261 | LEU | 262 | ALA | 0.000 | 0.147 | -0.147 | 0.147 |
| 132 | ILE | 219 | GLU | 0.910 | 0.763 | 0.147 | 0.147 |
| 332 | SER | 333 | PRO | 1.963 | 2.110 | -0.146 | 0.146 |
| 199 | ILE | 205 | PRO | 0.336 | 0.190 | 0.146 | 0.146 |
| 187 | PHE | 197 | THR | 0.226 | 0.081 | 0.145 | 0.145 |
| 93 | MET | 97 | ALA | 0.837 | 0.982 | -0.144 | 0.144 |
| 103 | CYS | 176 | ARG | 0.927 | 0.785 | 0.143 | 0.143 |
| 161 | LEU | 197 | THR | 0.142 | 0.000 | 0.142 | 0.142 |
| 90 | TYR | 106 | TRP | 0.000 | 0.141 | -0.141 | 0.141 |
| 57 | VAL | 509 | LEU | 0.340 | 0.198 | 0.141 | 0.141 |
| 472 | VAL | 476 | LEU | 0.080 | 0.221 | -0.141 | 0.141 |
| 483 | LEU | 486 | VAL | 0.141 | 0.000 | 0.141 | 0.141 |
| 143 | THR | 145 | LYS | 0.484 | 0.344 | 0.140 | 0.140 |
| 77 | LEU | 81 | ILE | 2.088 | 1.950 | 0.138 | 0.138 |
| 115 | ASN | 119 | MET | 2.053 | 2.190 | -0.137 | 0.137 |
| 163 | ALA | 168 | CYS | 0.152 | 0.017 | 0.135 | 0.135 |
| 435 | VAL | 439 | LYS | 1.470 | 1.335 | 0.134 | 0.134 |
| 507 | LYS | 512 | CYS | 0.134 | 0.000 | 0.134 | 0.134 |
| 458 | TYR | 461 | MET | 0.056 | 0.190 | -0.134 | 0.134 |
| 68 | TYR | 138 | TYR | 0.000 | 0.131 | -0.131 | 0.131 |
| 66 | ASN | 499 | ASN | 0.129 | 0.000 | 0.129 | 0.129 |
| 401 | LYS | 403 | MET | 0.000 | 0.128 | -0.128 | 0.128 |
| 33 | ILE | 37 | THR | 0.202 | 0.330 | -0.128 | 0.128 |
| 131 | SER | 138 | TYR | 2.902 | 2.775 | 0.126 | 0.126 |
| 107 | LEU | 184 | GLN | 2.006 | 2.132 | -0.126 | 0.126 |
| 234 | ASP | 238 | LYS | 0.000 | 0.126 | -0.126 | 0.126 |
| 167 | LEU | 168 | CYS | 0.110 | 0.232 | -0.122 | 0.122 |
| 69 | LEU | 124 | ILE | 0.603 | 0.481 | 0.122 | 0.122 |
| 121 | LEU | 124 | ILE | 0.206 | 0.084 | 0.121 | 0.121 |
| 476 | LEU | 479 | LEU | 0.043 | 0.164 | -0.120 | 0.120 |
| 149 | ILE | 153 | LEU | 0.639 | 0.520 | 0.119 | 0.119 |
| 125 | SER | 216 | ILE | 0.321 | 0.439 | -0.118 | 0.118 |
| 354 | SER | 355 | ASP | 0.697 | 0.579 | 0.118 | 0.118 |
| 188 | LEU | 477 | TRP | 0.118 | 0.000 | 0.118 | 0.118 |
| 86 | LEU | 96 | TRP | 0.901 | 0.785 | 0.116 | 0.116 |
| 86 | LEU | 98 | LEU | 0.533 | 0.418 | 0.115 | 0.115 |
| 178 | VAL | 183 | CYS | 0.170 | 0.055 | 0.115 | 0.115 |
| 49 | VAL | 53 | ILE | 1.135 | 1.020 | 0.114 | 0.114 |
| 434 | LEU | 437 | GLU | 0.113 | 0.000 | 0.113 | 0.113 |
| 472 | VAL | 473 | PRO | 0.804 | 0.691 | 0.113 | 0.113 |
| 258 | ARG | 259 | PRO | 0.244 | 0.131 | 0.113 | 0.113 |
| 82 | PHE | 86 | LEU | 0.221 | 0.108 | 0.112 | 0.112 |
| 107 | LEU | 481 | TYR | 0.213 | 0.102 | 0.111 | 0.111 |
| 126 | PHE | 212 | LEU | 4.366 | 4.472 | -0.106 | 0.106 |
| 98 | LEU | 102 | ALA | 0.675 | 0.569 | 0.106 | 0.106 |
| 39 | VAL | 43 | ILE | 0.646 | 0.751 | -0.105 | 0.105 |
| 505 | THR | 509 | LEU | 0.461 | 0.566 | -0.105 | 0.105 |
| 67 | TYR | 151 | ILE | 2.000 | 2.104 | -0.104 | 0.104 |
| 89 | THR | 93 | MET | 0.988 | 1.092 | -0.103 | 0.103 |
| 159 | PHE | 163 | ALA | 1.629 | 1.732 | -0.103 | 0.103 |
| 475 | THR | 476 | LEU | 0.103 | 0.000 | 0.103 | 0.103 |
| 48 | ASN | 52 | MET | 0.103 | 0.000 | 0.103 | 0.103 |
| 471 | CYS | 472 | VAL | 0.000 | 0.103 | -0.103 | 0.103 |
| 464 | VAL | 472 | VAL | 0.531 | 0.634 | -0.102 | 0.102 |
| 443 | THR | 499 | ASN | 0.000 | 0.102 | -0.102 | 0.102 |
| 213 | TYR | 216 | ILE | 0.896 | 0.999 | -0.102 | 0.102 |
| 460 | ILE | 464 | VAL | 0.161 | 0.263 | -0.102 | 0.102 |
| 477 | TRP | 481 | TYR | 0.102 | 0.000 | 0.102 | 0.102 |
| 469 | ASP | 470 | LYS | 0.100 | 0.000 | 0.100 | 0.100 |
| 45 | ILE | 85 | ASN | 0.100 | 0.000 | 0.100 | 0.100 |
| 366 | ALA | 367 | ALA | 0.221 | 0.321 | -0.100 | 0.100 |
| 205 | PRO | 209 | MET | 0.000 | 0.097 | -0.097 | 0.097 |
| 503 | ARG | 507 | LYS | 0.097 | 0.000 | 0.097 | 0.097 |
| 50 | LEU | 510 | LEU | 0.537 | 0.633 | -0.096 | 0.096 |
| 58 | ASN | 59 | SER | 0.129 | 0.224 | -0.095 | 0.095 |
| 373 | SER | 374 | GLN | 0.000 | 0.095 | -0.095 | 0.095 |
| 117 | SER | 487 | ASN | 0.280 | 0.187 | 0.094 | 0.094 |
| 219 | GLU | 222 | LYS | 0.227 | 0.134 | 0.092 | 0.092 |
| 104 | ASP | 167 | LEU | 1.975 | 1.883 | 0.092 | 0.092 |
| 443 | THR | 444 | LEU | 0.000 | 0.091 | -0.091 | 0.091 |
| 202 | PHE | 456 | THR | 2.767 | 2.678 | 0.089 | 0.089 |
| 48 | ASN | 77 | LEU | 0.992 | 1.081 | -0.089 | 0.089 |
| 169 | TRP | 172 | LEU | 0.000 | 0.087 | -0.087 | 0.087 |
| 295 | ALA | 296 | LYS | 0.086 | 0.000 | 0.086 | 0.086 |
| 130 | PHE | 133 | THR | 0.264 | 0.178 | 0.086 | 0.086 |
| 443 | THR | 494 | CYS | 0.000 | 0.085 | -0.085 | 0.085 |
| 82 | PHE | 109 | LEU | 1.345 | 1.429 | -0.084 | 0.084 |
| 83 | SER | 110 | ASP | 6.536 | 6.452 | 0.084 | 0.084 |
| 168 | CYS | 171 | TYR | 0.000 | 0.082 | -0.082 | 0.082 |
| 88 | THR | 92 | LEU | 1.046 | 1.127 | -0.082 | 0.082 |
| 69 | LEU | 496 | ALA | 0.082 | 0.000 | 0.082 | 0.082 |
| 206 | VAL | 209 | MET | 0.000 | 0.080 | -0.080 | 0.080 |
| 220 | THR | 434 | LEU | 0.000 | 0.080 | -0.080 | 0.080 |
| 134 | ARG | 137 | THR | 1.401 | 1.480 | -0.079 | 0.079 |
| 248 | ALA | 249 | LEU | 0.078 | 0.000 | 0.078 | 0.078 |
| 507 | LYS | 511 | LEU | 0.077 | 0.000 | 0.077 | 0.077 |
| 75 | ALA | 113 | ALA | 1.171 | 1.248 | -0.077 | 0.077 |
| 194 | THR | 462 | VAL | 1.153 | 1.229 | -0.076 | 0.076 |
| 153 | LEU | 156 | LEU | 0.074 | 0.000 | 0.074 | 0.074 |
| 74 | CYS | 155 | TRP | 0.215 | 0.287 | -0.072 | 0.072 |
| 529 | SER | 530 | LYS | 0.072 | 0.000 | 0.072 | 0.072 |
| 164 | PRO | 169 | TRP | 0.376 | 0.305 | 0.071 | 0.071 |
| 34 | ALA | 88 | THR | 0.027 | 0.098 | -0.071 | 0.071 |
| 104 | ASP | 170 | GLN | 3.014 | 2.943 | 0.071 | 0.071 |
| 69 | LEU | 444 | LEU | 0.000 | 0.071 | -0.071 | 0.071 |
| 41 | SER | 45 | ILE | 0.472 | 0.541 | -0.070 | 0.070 |
| 424 | HIS | 427 | THR | 0.000 | 0.070 | -0.070 | 0.070 |
| 110 | ASP | 111 | TYR | 0.155 | 0.085 | 0.070 | 0.070 |
| 15 | THR | 17 | VAL | 0.000 | 0.069 | -0.069 | 0.069 |
| 45 | ILE | 80 | GLY | 2.393 | 2.462 | -0.069 | 0.069 |
| 202 | PHE | 206 | VAL | 2.699 | 2.630 | 0.068 | 0.068 |
| 79 | ILE | 488 | SER | 2.671 | 2.604 | 0.067 | 0.067 |
| 103 | CYS | 107 | LEU | 0.894 | 0.961 | -0.067 | 0.067 |
| 118 | VAL | 451 | PHE | 1.890 | 1.824 | 0.066 | 0.066 |
| 454 | THR | 480 | GLY | 0.042 | 0.107 | -0.065 | 0.065 |
| 44 | THR | 48 | ASN | 0.670 | 0.605 | 0.064 | 0.064 |
| 90 | TYR | 91 | ILE | 0.000 | 0.063 | -0.063 | 0.063 |
| 87 | TYR | 106 | TRP | 3.026 | 2.963 | 0.063 | 0.063 |
| 162 | TRP | 166 | ILE | 0.533 | 0.596 | -0.063 | 0.063 |
| 118 | VAL | 209 | MET | 0.000 | 0.060 | -0.060 | 0.060 |
| 78 | ILE | 113 | ALA | 0.889 | 0.829 | 0.060 | 0.060 |
| 129 | TYR | 134 | ARG | 0.000 | 0.060 | -0.060 | 0.060 |
| 474 | VAL | 478 | HIS | 1.811 | 1.752 | 0.059 | 0.059 |
| 80 | GLY | 85 | ASN | 2.397 | 2.339 | 0.058 | 0.058 |
| 180 | LEU | 181 | ASP | 0.000 | 0.058 | -0.058 | 0.058 |
| 431 | ARG | 435 | VAL | 0.000 | 0.058 | -0.058 | 0.058 |
| 495 | TYR | 503 | ARG | 0.000 | 0.058 | -0.058 | 0.058 |
| 225 | LYS | 229 | ASP | 0.000 | 0.058 | -0.058 | 0.058 |
| 521 | GLU | 525 | TRP | 0.058 | 0.000 | 0.058 | 0.058 |
| 111 | TYR | 166 | ILE | 1.143 | 1.087 | 0.056 | 0.056 |
| 158 | SER | 163 | ALA | 0.011 | 0.065 | -0.054 | 0.054 |
| 23 | GLU | 25 | HIS | 0.000 | 0.053 | -0.053 | 0.053 |
| 55 | PHE | 502 | PHE | 0.053 | 0.000 | 0.053 | 0.053 |
| 162 | TRP | 187 | PHE | 1.125 | 1.177 | -0.052 | 0.052 |
| 36 | VAL | 40 | VAL | 0.000 | 0.052 | -0.052 | 0.052 |
| 374 | GLN | 376 | CYS | 0.052 | 0.000 | 0.052 | 0.052 |
| 46 | VAL | 50 | LEU | 0.701 | 0.650 | 0.051 | 0.051 |
| 117 | SER | 488 | SER | 0.050 | 0.000 | 0.050 | 0.050 |
| 116 | ALA | 158 | SER | 4.060 | 4.110 | -0.050 | 0.050 |
| 78 | ILE | 109 | LEU | 1.563 | 1.512 | 0.050 | 0.050 |
| 221 | GLU | 224 | THR | 0.050 | 0.000 | 0.050 | 0.050 |
| 163 | ALA | 167 | LEU | 0.141 | 0.092 | 0.049 | 0.049 |
| 59 | SER | 60 | GLN | 0.129 | 0.178 | -0.049 | 0.049 |
| 107 | LEU | 111 | TYR | 0.246 | 0.199 | 0.048 | 0.048 |
| 79 | ILE | 117 | SER | 0.047 | 0.000 | 0.047 | 0.047 |
| 456 | THR | 460 | ILE | 1.239 | 1.286 | -0.047 | 0.047 |
| 442 | GLN | 445 | SER | 0.000 | 0.046 | -0.046 | 0.046 |
| 51 | VAL | 502 | PHE | 0.998 | 0.954 | 0.044 | 0.044 |
| 204 | ILE | 208 | VAL | 0.154 | 0.111 | 0.044 | 0.044 |
| 191 | PRO | 192 | THR | 0.043 | 0.000 | 0.043 | 0.043 |
| 116 | ALA | 120 | ASN | 0.103 | 0.145 | -0.042 | 0.042 |
| 451 | PHE | 456 | THR | 0.009 | 0.050 | -0.041 | 0.041 |
| 103 | CYS | 182 | GLU | 0.067 | 0.107 | -0.041 | 0.041 |
| 153 | LEU | 157 | ILE | 0.000 | 0.040 | -0.040 | 0.040 |
| 465 | SER | 466 | THR | 0.000 | 0.039 | -0.039 | 0.039 |
| 501 | THR | 505 | THR | 0.084 | 0.045 | 0.039 | 0.039 |
| 129 | TYR | 215 | ARG | 3.860 | 3.899 | -0.039 | 0.039 |
| 139 | ARG | 142 | ARG | 0.039 | 0.000 | 0.039 | 0.039 |
| 445 | SER | 449 | LEU | 0.039 | 0.000 | 0.039 | 0.039 |
| 198 | ALA | 459 | ASN | 0.081 | 0.042 | 0.038 | 0.038 |
| 119 | MET | 122 | LEU | 0.304 | 0.342 | -0.038 | 0.038 |
| 32 | THR | 36 | VAL | 0.091 | 0.128 | -0.037 | 0.037 |
| 64 | VAL | 68 | TYR | 0.000 | 0.036 | -0.036 | 0.036 |
| 134 | ARG | 138 | TYR | 0.490 | 0.524 | -0.034 | 0.034 |
| 54 | SER | 502 | PHE | 1.111 | 1.076 | 0.034 | 0.034 |
| 120 | ASN | 155 | TRP | 1.062 | 1.095 | -0.033 | 0.033 |
| 103 | CYS | 184 | GLN | 0.279 | 0.312 | -0.033 | 0.033 |
| 462 | VAL | 465 | SER | 0.032 | 0.000 | 0.032 | 0.032 |
| 78 | ILE | 83 | SER | 0.933 | 0.902 | 0.031 | 0.031 |
| 64 | VAL | 142 | ARG | 3.432 | 3.402 | 0.031 | 0.031 |
| 79 | ILE | 485 | TYR | 1.777 | 1.748 | 0.030 | 0.030 |
| 491 | ASN | 492 | PRO | 0.898 | 0.927 | -0.029 | 0.029 |
| 159 | PHE | 160 | ILE | 0.145 | 0.117 | 0.028 | 0.028 |
| 364 | SER | 365 | PRO | 0.810 | 0.784 | 0.026 | 0.026 |
| 380 | LYS | 382 | ARG | 0.026 | 0.000 | 0.026 | 0.026 |
| 134 | ARG | 135 | PRO | 0.115 | 0.089 | 0.026 | 0.026 |
| 456 | THR | 457 | PRO | 0.957 | 0.982 | -0.026 | 0.026 |
| 123 | VAL | 154 | ALA | 0.145 | 0.120 | 0.025 | 0.025 |
| 121 | LEU | 209 | MET | 2.166 | 2.141 | 0.025 | 0.025 |
| 130 | PHE | 135 | PRO | 0.197 | 0.174 | 0.024 | 0.024 |
| 169 | TRP | 174 | GLY | 0.023 | 0.000 | 0.023 | 0.023 |
| 210 | THR | 214 | CYS | 0.291 | 0.269 | 0.023 | 0.023 |
| 132 | ILE | 437 | GLU | 0.000 | 0.022 | -0.022 | 0.022 |
| 216 | ILE | 220 | THR | 0.320 | 0.341 | -0.021 | 0.021 |
| 68 | TYR | 150 | MET | 1.114 | 1.135 | -0.021 | 0.021 |
| 227 | LEU | 434 | LEU | 0.000 | 0.020 | -0.020 | 0.020 |
| 412 | GLU | 413 | PRO | 0.066 | 0.086 | -0.020 | 0.020 |
| 473 | PRO | 476 | LEU | 1.660 | 1.679 | -0.019 | 0.019 |
| 204 | ILE | 205 | PRO | 1.456 | 1.475 | -0.018 | 0.018 |
| 382 | ARG | 384 | VAL | 0.018 | 0.000 | 0.018 | 0.018 |
| 193 | ILE | 197 | THR | 0.351 | 0.369 | -0.017 | 0.017 |
| 42 | LEU | 46 | VAL | 0.668 | 0.651 | 0.017 | 0.017 |
| 71 | SER | 155 | TRP | 3.183 | 3.166 | 0.016 | 0.016 |
| 27 | LEU | 31 | ILE | 0.936 | 0.921 | 0.016 | 0.016 |
| 67 | TYR | 147 | ALA | 0.038 | 0.022 | 0.016 | 0.016 |
| 116 | ALA | 154 | ALA | 0.061 | 0.076 | -0.015 | 0.015 |
| 522 | LYS | 526 | GLN | 0.015 | 0.000 | 0.015 | 0.015 |
| 255 | ARG | 256 | CYS | 0.013 | 0.000 | 0.013 | 0.013 |
| 179 | PRO | 184 | GLN | 0.035 | 0.022 | 0.013 | 0.013 |
| 145 | LYS | 149 | ILE | 0.011 | 0.000 | 0.011 | 0.011 |
| 138 | TYR | 139 | ARG | 0.011 | 0.000 | 0.011 | 0.011 |
| 342 | THR | 343 | GLU | 1.893 | 1.903 | -0.010 | 0.010 |
| 44 | THR | 80 | GLY | 1.026 | 1.016 | 0.009 | 0.009 |
| 121 | LEU | 213 | TYR | 0.008 | 0.000 | 0.008 | 0.008 |
| 407 | PHE | 408 | PRO | 0.498 | 0.506 | -0.008 | 0.008 |
| 224 | THR | 438 | ARG | 0.000 | 0.008 | -0.008 | 0.008 |
| 136 | LEU | 137 | THR | 0.007 | 0.000 | 0.007 | 0.007 |
| 263 | GLN | 264 | ARG | 1.333 | 1.326 | 0.007 | 0.007 |
| 78 | ILE | 82 | PHE | 0.306 | 0.300 | 0.006 | 0.006 |
| 299 | GLN | 300 | LEU | 0.006 | 0.000 | 0.006 | 0.006 |
| 206 | VAL | 210 | THR | 0.395 | 0.391 | 0.005 | 0.005 |
| 163 | ALA | 164 | PRO | 0.825 | 0.820 | 0.004 | 0.004 |
| 458 | TYR | 480 | GLY | 2.261 | 2.257 | 0.004 | 0.004 |
| 10 | THR | 12 | VAL | 0.000 | 0.004 | -0.004 | 0.004 |
| 43 | ILE | 489 | THR | 1.186 | 1.184 | 0.003 | 0.003 |
| 57 | VAL | 58 | ASN | 0.149 | 0.146 | 0.003 | 0.003 |
| 486 | VAL | 489 | THR | 0.000 | 0.003 | -0.003 | 0.003 |
| 499 | ASN | 500 | ARG | 0.002 | 0.000 | 0.002 | 0.002 |
| 51 | VAL | 69 | LEU | 0.298 | 0.296 | 0.002 | 0.002 |
| 162 | TRP | 185 | ILE | 0.189 | 0.189 | 0.001 | 0.001 |
| 22 | LEU | 474 | VAL | 0.000 | 0.000 | -0.000 | 0.000 |
| 320 | VAL | 322 | GLN | 0.016 | 0.016 | -0.000 | 0.000 |
RRCS change distribution
-0.13
Mean ΔRRCS
1.67
Std Dev
-0.04
Median
Magnitude classification
21
High (|Δ| ≥ 5.0)
57
Medium (2.3 ≤ |Δ| < 5.0)
719
Low (|Δ| < 2.3)
Methods
RRCS analysis. Residue-Residue Contact Scores (RRCS) were calculated for each conformational state based on inter-atomic distances. Changes (ΔRRCS) were computed by comparing active and inactive structures predicted by AlphaFold multistate (del Alamo et al., 2022).
Significance threshold. |ΔRRCS| ≥ 2.33, computed as max(mean(|Δ|) + σ, 0.2). The 0.2 floor ensures receptors with very small overall change still surface their largest contacts.
Variant data. Population frequencies from gnomAD v4; pathogenicity predictions from AlphaMissense; conservation from ProtVar / UniProt.
Structural annotation. TM domain boundaries and generic numbering from GPCRdb.
What is RRCS?
Residue-Residue Contact Score (RRCS) measures how strongly two amino acids interact in a protein structure. When a protein changes shape (from inactive to active), these contact strengths change.
ΔRRCS (Delta RRCS) shows the difference in contact strength between states:
- Positive ΔRRCS. Contact is stronger in the active state.
- Negative ΔRRCS. Contact is stronger in the inactive state.
- Large |ΔRRCS|. Significant structural rearrangement.
Residues with large RRCS changes are critical for protein function. Mutations at these positions may disrupt the protein's ability to change shape properly.
Pathogenicity predictions
AlphaMissense predicts whether a mutation harms protein function:
- Pathogenic (score ≥ 0.564): mutation likely damages function.
- Ambiguous (0.34–0.563): effect uncertain.
- Benign (< 0.34): mutation likely tolerated.
Conservation & population data
Conservation score. How well-preserved a position is across species (0 = variable, 1 = conserved). High conservation suggests the position is critical for function.
Allele frequency. How often a variant appears in the population. Rare variants (low frequency) may be more likely to be harmful.
Sources: AlphaFold multistate · RRCS · gnomAD v4 · AlphaMissense · GPCRdb · UniProt / ProtVar