ADA1B
745
Total Contact Pairs
82
Significant Changes
11.57
Max Increase
-7.84
Max Decrease
Interactive snake plot
GPCRdb-style topology. Click the view buttons to recolor residues by different data layers. Toggle contact links to draw significant residue-residue contacts as arcs. Click loop labels (ICL/ECL) to expand or collapse loop regions.
Color convention: blue = active-favoring, red = inactive-favoring.
Top 100 conformational changes
Residue pairs with the largest RRCS changes between active and inactive states. GPCRdb numbering in parentheses. Highlighted rows exceed the significance threshold.
| Rank | Residue 1 | Residue 2 | Active RRCS | Inactive RRCS | ΔRRCS | Magnitude |
|---|---|---|---|---|---|---|
| 1 | ARG467 | HIS468 | 11.572 | 0.000 | +11.572 | HIGH |
| 2 | HIS468 | ASP469 | 10.241 | 0.000 | +10.241 | HIGH |
| 3 | LYS243 | GLU250 | 0.000 | 7.840 | -7.840 | HIGH |
| 4 | THR233 (5.68x68) | GLU289 (6.30x30) | 7.771 | 0.000 | +7.771 | HIGH |
| 5 | ARG115 (3.22x22) | ASP192 | 1.800 | 9.341 | -7.541 | HIGH |
| 6 | ARG148 (3.55x55) | TYR149 (3.56x56) | 4.241 | 11.182 | -6.941 | HIGH |
| 7 | THR80 (2.39x39) | ARG143 (3.50x50) | 0.000 | 6.808 | -6.808 | HIGH |
| 8 | GLU186 | GLU200 (5.35x36) | 8.587 | 15.148 | -6.560 | HIGH |
| 9 | THR295 (6.36x36) | SER351 (7.56x56) | 0.851 | 7.049 | -6.199 | HIGH |
| 10 | ASN236 (5.71x71) | VAL241 | 0.000 | 6.095 | -6.095 | HIGH |
| 11 | GLN506 | PRO507 | 0.000 | 6.063 | -6.063 | HIGH |
| 12 | ASP91 (2.50x50) | SER341 (7.46x46) | 9.193 | 3.188 | +6.006 | HIGH |
| 13 | GLU238 (5.73x73) | PHE286 (6.27x27) | 5.960 | 0.000 | +5.960 | HIGH |
| 14 | PHE286 (6.27x27) | LYS290 (6.31x31) | 5.894 | 0.000 | +5.894 | HIGH |
| 15 | ASP142 (3.49x49) | ARG143 (3.50x50) | 0.000 | 5.117 | -5.117 | HIGH |
| 16 | THR234 (5.69x69) | GLU289 (6.30x30) | 5.062 | 0.000 | +5.062 | HIGH |
| 17 | PHE303 (6.44x44) | ASN340 (7.45x45) | 0.000 | 4.920 | -4.920 | MED |
| 18 | ASN340 (7.45x45) | ASN344 (7.49x49) | 4.844 | 0.000 | +4.844 | MED |
| 19 | TRP307 (6.48x48) | GLY337 (7.42x41) | 3.821 | 8.646 | -4.825 | MED |
| 20 | ARG361 (8.56x56) | GLN366 | 0.000 | 4.778 | -4.778 | MED |
| 21 | ASN73 (1.60x60) | HIS75 (12.49x49) | 4.704 | 0.000 | +4.704 | MED |
| 22 | GLY240 | ILE255 | 0.000 | 4.703 | -4.703 | MED |
| 23 | GLU244 | SER278 | 4.694 | 0.000 | +4.694 | MED |
| 24 | PHE310 (6.51x51) | LEU314 (6.55x55) | 1.001 | 5.542 | -4.540 | MED |
| 25 | ILE133 (3.40x40) | TRP307 (6.48x48) | 4.522 | 0.000 | +4.522 | MED |
| 26 | PHE303 (6.44x44) | TRP307 (6.48x48) | 2.469 | 6.920 | -4.451 | MED |
| 27 | PRO349 (7.54x54) | PHE355 (8.50x50) | 5.114 | 0.708 | +4.406 | MED |
| 28 | ARG143 (3.50x50) | TYR223 (5.58x58) | 4.328 | 0.000 | +4.328 | MED |
| 29 | GLU250 | ARG254 | 4.305 | 0.000 | +4.305 | MED |
| 30 | TYR223 (5.58x58) | LEU296 (6.37x37) | 2.317 | 6.549 | -4.233 | MED |
| 31 | TYR144 (3.51x51) | ARG148 (3.55x55) | 2.052 | 6.262 | -4.211 | MED |
| 32 | GLU186 | GLU199 | 0.000 | 4.208 | -4.208 | MED |
| 33 | ILE133 (3.40x40) | PHE303 (6.44x44) | 1.379 | 5.454 | -4.074 | MED |
| 34 | GLU244 | LYS282 | 6.410 | 2.337 | +4.074 | MED |
| 35 | PRO349 (7.54x54) | LYS356 (8.51x51) | 0.465 | 4.489 | -4.024 | MED |
| 36 | LEU136 (3.43x43) | TYR348 (7.53x53) | 3.958 | 0.000 | +3.958 | MED |
| 37 | SER267 | THR268 | 0.000 | 3.787 | -3.787 | MED |
| 38 | LEU237 (5.72x72) | LYS285 (6.26x26) | 3.784 | 0.000 | +3.784 | MED |
| 39 | SER150 (34.50x50) | PHE286 (6.27x27) | 0.000 | 3.742 | -3.742 | MED |
| 40 | TYR144 (3.51x51) | MET222 (5.57x57) | 4.215 | 0.546 | +3.669 | MED |
| 41 | HIS75 (12.49x49) | LEU76 (12.50x50) | 3.536 | 0.000 | +3.536 | MED |
| 42 | GLY179 (4.59x59) | TRP184 (4.64x64) | 5.755 | 2.384 | +3.371 | MED |
| 43 | ILE60 (1.47x47) | PHE96 (2.55x55) | 0.559 | 3.888 | -3.328 | MED |
| 44 | TYR348 (7.53x53) | PHE355 (8.50x50) | 0.000 | 3.314 | -3.314 | MED |
| 45 | ARG77 (12.51x51) | TYR82 (2.41x41) | 3.341 | 0.102 | +3.239 | MED |
| 46 | ILE84 (2.43x43) | TYR348 (7.53x53) | 0.262 | 3.482 | -3.220 | MED |
| 47 | ILE176 (4.56x56) | TYR203 (5.38x39) | 3.146 | 0.000 | +3.146 | MED |
| 48 | SER402 | ARG403 | 0.347 | 3.311 | -2.964 | MED |
| 49 | ASP409 | SER410 | 2.958 | 0.000 | +2.958 | MED |
| 50 | TYR223 (5.58x58) | GLY297 | 0.000 | 2.884 | -2.884 | MED |
| 51 | TRP307 (6.48x48) | ASN340 (7.45x45) | 6.198 | 3.343 | +2.855 | MED |
| 52 | PRO180 | LYS185 | 2.933 | 0.110 | +2.824 | MED |
| 53 | ASN2 | PRO3 | 0.084 | 2.903 | -2.819 | MED |
| 54 | LYS404 | ASP405 | 0.000 | 2.802 | -2.802 | MED |
| 55 | ARG115 (3.22x22) | LYS185 | 2.789 | 0.000 | +2.789 | MED |
| 56 | SER352 | GLU354 | 3.474 | 0.717 | +2.757 | MED |
| 57 | TYR223 (5.58x58) | VAL300 | 2.088 | 4.812 | -2.724 | MED |
| 58 | LEU105 | TYR110 | 3.593 | 0.870 | +2.723 | MED |
| 59 | LYS285 (6.26x26) | GLU289 (6.30x30) | 2.794 | 0.092 | +2.703 | MED |
| 60 | ARG115 (3.22x22) | ALA188 | 2.933 | 0.232 | +2.701 | MED |
| 61 | HIS75 (12.49x49) | ARG361 (8.56x56) | 2.694 | 0.000 | +2.694 | MED |
| 62 | ILE133 (3.40x40) | PHE212 | 0.122 | 2.813 | -2.691 | MED |
| 63 | SER484 | PRO485 | 0.511 | 3.164 | -2.653 | MED |
| 64 | ASN63 | ASP91 (2.50x50) | 4.624 | 1.976 | +2.649 | MED |
| 65 | PRO180 | THR198 | 2.604 | 0.000 | +2.604 | MED |
| 66 | LEU40 | ARG44 | 2.596 | 0.000 | +2.596 | MED |
| 67 | ARG115 (3.22x22) | ASP119 | 2.582 | 0.000 | +2.582 | MED |
| 68 | PHE55 | PHE339 | 1.421 | 3.996 | -2.575 | MED |
| 69 | GLU186 | THR198 | 0.000 | 2.546 | -2.546 | MED |
| 70 | PHE55 | TYR338 | 0.000 | 2.517 | -2.517 | MED |
| 71 | LEU136 (3.43x43) | PHE303 (6.44x44) | 1.490 | 3.994 | -2.505 | MED |
| 72 | GLU354 | ARG357 | 0.000 | 2.478 | -2.478 | MED |
| 73 | ASN63 | ALA88 | 5.506 | 3.038 | +2.469 | MED |
| 74 | LEU151 | GLU244 | 0.000 | 2.465 | -2.465 | MED |
| 75 | ASP191 | LYS193 | 2.462 | 0.000 | +2.462 | MED |
| 76 | ASN81 | PHE355 (8.50x50) | 3.322 | 0.948 | +2.374 | MED |
| 77 | THR234 (5.69x69) | PHE286 (6.27x27) | 2.374 | 0.000 | +2.374 | MED |
| 78 | ARG143 (3.50x50) | LEU296 (6.37x37) | 0.000 | 2.352 | -2.352 | MED |
| 79 | SER425 | PRO426 | 2.726 | 0.390 | +2.336 | MED |
| 80 | SER417 | GLN418 | 2.334 | 0.000 | +2.334 | MED |
| 81 | PHE55 | CYS342 | 1.041 | 3.372 | -2.330 | MED |
| 82 | LEU237 (5.72x72) | LEU283 | 0.000 | 2.290 | -2.290 | MED |
| 83 | PHE58 | CYS342 | 1.891 | 4.167 | -2.277 | LOW |
| 84 | ILE219 | PHE303 (6.44x44) | 2.254 | 0.000 | +2.254 | LOW |
| 85 | SER150 (34.50x50) | GLU289 (6.30x30) | 0.000 | 2.208 | -2.208 | LOW |
| 86 | ILE228 | ARG232 | 2.190 | 0.000 | +2.190 | LOW |
| 87 | ASP91 (2.50x50) | ASN344 (7.49x49) | 4.744 | 2.581 | +2.163 | LOW |
| 88 | MET242 | PHE286 (6.27x27) | 0.000 | 2.144 | -2.144 | LOW |
| 89 | PHE212 | PHE311 | 14.751 | 16.853 | -2.102 | LOW |
| 90 | THR234 (5.69x69) | LYS290 (6.31x31) | 0.123 | 2.184 | -2.062 | LOW |
| 91 | GLU244 | ILE279 | 2.058 | 0.000 | +2.058 | LOW |
| 92 | LEU515 | ALA516 | 0.000 | 2.031 | -2.031 | LOW |
| 93 | CYS496 | GLU497 | 2.029 | 0.000 | +2.029 | LOW |
| 94 | ARG143 (3.50x50) | TYR348 (7.53x53) | 2.025 | 0.000 | +2.025 | LOW |
| 95 | ILE362 | CYS367 | 0.000 | 1.996 | -1.996 | LOW |
| 96 | SER511 | ASN512 | 0.000 | 1.982 | -1.982 | LOW |
| 97 | PRO3 | ASP4 | 0.000 | 1.978 | -1.978 | LOW |
| 98 | ASN34 | THR36 | 1.937 | 0.000 | +1.937 | LOW |
| 99 | ARG143 (3.50x50) | TYR153 | 0.000 | 1.935 | -1.935 | LOW |
| 100 | LEU151 | LYS282 | 0.000 | 1.913 | -1.913 | LOW |
Transmembrane domain analysis
Active-favoring residues form stronger contacts in the active state (positive ΔRRCS). Inactive-favoring residues form stronger contacts in the inactive state (negative ΔRRCS). Only residues with |ΔRRCS| ≥ 2.28 are shown (per-receptor significance threshold = max(mean(|Δ|) + σ, 0.2)).
| Segment | Range | Active-favoring residues | Count | Inactive-favoring residues | Count |
|---|---|---|---|---|---|
| TM1 | 60-73 | 73 (4.7) | 1 | 60 (-3.3) | 1 |
| TM2 | 80-96 | 91 (6.0), 82 (3.2) | 2 | 80 (-6.8), 96 (-3.3), 84 (-3.2) | 3 |
| TM3 | 115-149 | 133 (4.5), 136 (4.0) | 2 | 115 (-7.5), 148 (-6.9), 149 (-6.9), 143 (-6.8), 142 (-5.1), 144 (-4.2) | 6 |
| TM4 | 176-184 | 179 (3.4), 184 (3.4), 176 (3.1) | 3 | - | 0 |
| TM5 | 200-238 | 233 (7.8), 238 (6.0), 234 (5.1), 223 (4.3), 237 (3.8), 222 (3.7), 203 (3.1) | 7 | 200 (-6.6), 236 (-6.1) | 2 |
| TM6 | 285-314 | 289 (7.8), 286 (6.0), 290 (5.9), 285 (3.8) | 4 | 295 (-6.2), 303 (-4.9), 307 (-4.8), 310 (-4.5), 314 (-4.5), 296 (-4.2) | 6 |
| TM7 | 337-351 | 341 (6.0), 344 (4.8), 349 (4.4), 348 (4.0) | 4 | 351 (-6.2), 340 (-4.9), 337 (-4.8) | 3 |
| Intracellular / Extracellular loops & H8 | |||||
| ICL1 | 75-77 | 75 (4.7), 76 (3.5), 77 (3.2) | 3 | - | 0 |
| ICL2 | 150-150 | - | 0 | 150 (-3.7) | 1 |
| ICL3 | 240-282 | 244 (4.7), 278 (4.7), 254 (4.3), 282 (4.1) | 4 | 243 (-7.8), 250 (-7.8), 241 (-6.1), 240 (-4.7), 255 (-4.7), 267 (-3.8), 268 (-3.8) | 7 |
| ECL1 | - | - | 0 | - | 0 |
| ECL2 | 186-199 | - | 0 | 192 (-7.5), 186 (-6.6), 199 (-4.2) | 3 |
| ECL3 | - | - | 0 | - | 0 |
| H8 | 355-361 | 355 (4.4) | 1 | 361 (-4.8), 356 (-4.0) | 2 |
Interactive visualizations
ΔRRCS distribution
Active vs inactive comparison
Residue-wise changes
TM domain breakdown
Variants of interest
89 variants mapped to contact positions, sorted by |ΔRRCS| impact.
| Position | Protein change | DNA change | Allele freq | Het / Hom | ΔRRCS | AM score | Pathogenicity | Conservation | dbSNP |
|---|---|---|---|---|---|---|---|---|---|
| 467 | p.Arg467Ser | c.1399C>A | 7.75e-07 | 1 / 0 | 11.57 | 0.115 | BENIGN | 0.66 | |
| 468 | p.His468Arg | c.1403A>G | 7.73e-07 | 1 / 0 | 11.57 | 0.047 | BENIGN | 0.42 | rs1755891332 |
| 467 | p.Arg467Leu | c.1400G>T | 6.61e-06 | 1 / 0 | 11.57 | - | nan | - | rs1277910780 |
| 467 | p.Arg467His | c.1400G>A | 1.55e-06 | 2 / 0 | 11.57 | - | nan | - | rs1277910780 |
| 468 | p.His468Gln | c.1404C>G | 7.73e-07 | 1 / 0 | 11.57 | - | nan | - | rs1283756765 |
| 469 | p.Asp469Asn | c.1405G>A | 2.32e-06 | 3 / 0 | 10.24 | 0.108 | BENIGN | 0.76 | |
| 469 | p.Asp469Tyr | c.1405G>T | 7.73e-07 | 1 / 0 | 10.24 | - | nan | - | |
| 469 | p.Asp469Glu | c.1407C>A | 1.60e-05 | 21 / 0 | 10.24 | - | nan | - | rs1489591708 |
| 289 | p.Glu289Lys | c.865G>A | 6.84e-07 | 1 / 0 | 7.77 | 0.990 | PATHOGENIC | 0.88 | |
| 233 | p.Thr233Ile | c.698C>T | 6.84e-07 | 1 / 0 | 7.77 | 0.707 | PATHOGENIC | 0.48 | rs2113149926 |
| 233 | p.Thr233Asn | c.698C>A | 6.84e-07 | 1 / 0 | 7.77 | - | nan | - | |
| 115 | p.Arg115Trp | c.343C>T | 2.05e-06 | 3 / 0 | 7.54 | 0.533 | AMBIGUOUS | 0.57 | |
| 192 | p.Asp192Val | c.575A>T | 6.57e-06 | 1 / 0 | 7.54 | 0.157 | BENIGN | 0.54 | rs1754354319 |
| 115 | p.Arg115Gln | c.344G>A | 1.98e-05 | 29 / 0 | 7.54 | - | nan | - | rs1273352160 |
| 149 | p.Tyr149Cys | c.446A>G | 2.05e-06 | 3 / 0 | 6.94 | 0.536 | AMBIGUOUS | 0.49 | rs1419494240 |
| 148 | p.Arg148His | c.443G>A | 6.84e-07 | 1 / 0 | 6.94 | 0.280 | BENIGN | 0.51 | rs758643818 |
| 143 | p.Arg143Gly | c.427C>G | 6.84e-07 | 1 / 0 | 6.81 | 0.996 | PATHOGENIC | 0.98 | |
| 80 | p.Thr80Ile | c.239C>T | 2.05e-06 | 3 / 0 | 6.81 | 0.992 | PATHOGENIC | 0.85 | rs760038075 |
| 143 | p.Arg143His | c.428G>A | 7.53e-06 | 11 / 0 | 6.81 | - | nan | - | rs757479764 |
| 186 | p.Glu186Lys | c.556G>A | 1.37e-06 | 2 / 0 | 6.56 | 0.543 | AMBIGUOUS | 0.66 | rs1256293097 |
| 186 | p.Glu186Gly | c.557A>G | 8.89e-06 | 11 / 1 | 6.56 | - | nan | - | rs751944506 |
| 186 | p.Glu186Asp | c.558G>C | 1.37e-06 | 2 / 0 | 6.56 | - | nan | - | rs371731539 |
| 295 | p.Thr295Pro | c.883A>C | 6.57e-06 | 1 / 0 | 6.20 | 0.979 | PATHOGENIC | 0.92 | rs202097956 |
| 351 | p.Ser351Thr | c.1051T>A | 1.53e-06 | 2 / 0 | 6.20 | 0.407 | AMBIGUOUS | 0.44 | rs777674763 |
| 295 | p.Thr295Met | c.884C>T | 8.21e-06 | 12 / 0 | 6.20 | - | nan | - | rs758073042 |
| 351 | p.Ser351Pro | c.1051T>C | 7.66e-07 | 1 / 0 | 6.20 | - | nan | - | rs777674763 |
| 351 | p.Ser351Cys | c.1052C>G | 7.53e-07 | 1 / 0 | 6.20 | - | nan | - | rs749273189 |
| 241 | p.Val241Leu | c.721G>C | 1.37e-06 | 2 / 0 | 6.10 | 0.496 | AMBIGUOUS | 0.47 | |
| 506 | p.Gln506His | c.1518G>C | 7.46e-07 | 1 / 0 | 6.06 | 0.114 | BENIGN | 0.83 | |
| 507 | p.Pro507Ser | c.1519C>T | 7.46e-07 | 1 / 0 | 6.06 | 0.079 | BENIGN | 0.74 | rs1755895561 |
| 507 | p.Pro507Leu | c.1520C>T | 2.24e-06 | 3 / 0 | 6.06 | - | nan | - | |
| 507 | p.Pro507Arg | c.1520C>G | 1.34e-05 | 16 / 1 | 6.06 | - | nan | - | rs1458467133 |
| 91 | p.Asp91Asn | c.271G>A | 1.37e-06 | 2 / 0 | 6.01 | 0.995 | PATHOGENIC | 1.00 | rs761487660 |
| 238 | p.Glu238Gly | c.713A>G | 6.58e-06 | 1 / 0 | 5.96 | 0.794 | PATHOGENIC | 0.49 | rs756724973 |
| 290 | p.Lys290Arg | c.869A>G | 1.37e-06 | 2 / 0 | 5.89 | 0.366 | AMBIGUOUS | 0.75 | |
| 290 | p.Lys290Asn | c.870G>T | 6.84e-07 | 1 / 0 | 5.89 | - | nan | - | |
| 142 | p.Asp142His | c.424G>C | 6.57e-06 | 1 / 0 | 5.12 | 0.996 | PATHOGENIC | 0.95 | rs1370209486 |
| 234 | p.Thr234Ala | c.700A>G | 6.58e-06 | 1 / 0 | 5.06 | 0.436 | AMBIGUOUS | 0.64 | rs1422658619 |
| 234 | p.Thr234Ile | c.701C>T | 6.84e-07 | 1 / 0 | 5.06 | - | nan | - | |
| 234 | p.Thr234Asn | c.701C>A | 1.31e-05 | 2 / 0 | 5.06 | - | nan | - | rs1429458895 |
| 303 | p.Phe303Leu | c.907T>C | 6.85e-07 | 1 / 0 | 4.92 | 1.000 | PATHOGENIC | 0.95 | rs1214605078 |
| 344 | p.Asn344Asp | c.1030A>G | 7.60e-07 | 1 / 0 | 4.84 | 0.997 | PATHOGENIC | 0.97 | rs754540841 |
| 344 | p.Asn344Thr | c.1031A>C | 7.95e-07 | 1 / 0 | 4.84 | - | nan | - | |
| 307 | p.Trp307Arg | c.919T>C | 6.85e-07 | 1 / 0 | 4.83 | 0.999 | PATHOGENIC | 0.97 | |
| 366 | p.Gln366Leu | c.1097A>T | 1.16e-05 | 14 / 0 | 4.78 | 0.250 | BENIGN | 0.49 | |
| 361 | p.Arg361His | c.1082G>A | 9.13e-06 | 12 / 0 | 4.78 | 0.203 | BENIGN | 0.53 | rs770336712 |
| 366 | p.Gln366His | c.1098G>T | 1.02e-05 | 13 / 0 | 4.78 | - | nan | - | rs771591500 |
| 366 | p.Gln366His | c.1098G>C | 7.84e-07 | 1 / 0 | 4.78 | - | nan | - | rs771591500 |
| 75 | p.His75Tyr | c.223C>T | 2.74e-06 | 4 / 0 | 4.70 | 0.376 | AMBIGUOUS | 0.57 | rs377516133 |
| 73 | p.Asn73Asp | c.217A>G | 5.47e-06 | 8 / 0 | 4.70 | 0.368 | AMBIGUOUS | 0.52 | rs1256231726 |
| 73 | p.Asn73Ser | c.218A>G | 6.84e-07 | 1 / 0 | 4.70 | - | nan | - | rs1754336735 |
| 244 | p.Glu244Lys | c.730G>A | 6.84e-07 | 1 / 0 | 4.69 | 0.914 | PATHOGENIC | 0.51 | |
| 278 | p.Ser278Phe | c.833C>T | 6.57e-06 | 1 / 0 | 4.69 | 0.710 | PATHOGENIC | 0.60 | rs1382562383 |
| 244 | p.Glu244Val | c.731A>T | 6.58e-06 | 1 / 0 | 4.69 | - | nan | - | rs745539683 |
| 310 | p.Phe310Val | c.928T>G | 6.86e-07 | 1 / 0 | 4.54 | 0.987 | PATHOGENIC | 0.97 | |
| 314 | p.Leu314Val | c.940C>G | 3.45e-06 | 5 / 0 | 4.54 | 0.631 | PATHOGENIC | 0.71 | rs1305758864 |
| 133 | p.Ile133Thr | c.398T>C | 8.89e-06 | 13 / 0 | 4.52 | 0.993 | PATHOGENIC | 1.00 | |
| 355 | p.Phe355Leu | c.1065C>A | 3.02e-06 | 4 / 0 | 4.41 | 0.999 | PATHOGENIC | 0.90 | rs1755877182 |
| 349 | p.Pro349Thr | c.1045C>A | 6.59e-06 | 1 / 0 | 4.41 | 0.662 | PATHOGENIC | 0.56 | rs1221135849 |
| 223 | p.Tyr223His | c.667T>C | 6.84e-07 | 1 / 0 | 4.33 | 0.998 | PATHOGENIC | 0.99 | rs909145193 |
| 223 | p.Tyr223Phe | c.668A>T | 1.23e-05 | 18 / 0 | 4.33 | - | nan | - | rs1754357621 |
| 223 | p.Tyr223Cys | c.668A>G | 6.84e-07 | 1 / 0 | 4.33 | - | nan | - | |
| 296 | p.Leu296Val | c.886T>G | 6.84e-07 | 1 / 0 | 4.23 | 0.543 | AMBIGUOUS | 0.96 | |
| 296 | p.Leu296Phe | c.888G>C | 6.84e-07 | 1 / 0 | 4.23 | - | nan | - | |
| 144 | p.Tyr144Cys | c.431A>G | 6.84e-07 | 1 / 0 | 4.21 | 0.892 | PATHOGENIC | 0.97 | |
| 199 | p.Glu199Lys | c.595G>A | 1.37e-06 | 2 / 0 | 4.21 | 0.138 | BENIGN | 0.66 | rs1328289918 |
| 282 | p.Lys282Glu | c.844A>G | 6.84e-07 | 1 / 0 | 4.07 | 0.973 | PATHOGENIC | 0.67 | rs1190364353 |
| 356 | p.Lys356Glu | c.1066A>G | 7.59e-07 | 1 / 0 | 4.02 | 0.852 | PATHOGENIC | 0.73 | |
| 356 | p.Lys356Asn | c.1068G>T | 7.55e-07 | 1 / 0 | 4.02 | - | nan | - | |
| 136 | p.Leu136Gln | c.407T>A | 6.84e-07 | 1 / 0 | 3.96 | 0.998 | PATHOGENIC | 0.99 | |
| 348 | p.Tyr348His | c.1042T>C | 6.95e-06 | 9 / 0 | 3.96 | 0.997 | PATHOGENIC | 0.97 | |
| 348 | p.Tyr348Asp | c.1042T>G | 7.72e-07 | 1 / 0 | 3.96 | - | nan | - | |
| 268 | p.Thr268Ala | c.802A>G | 2.05e-06 | 3 / 0 | 3.79 | 0.063 | BENIGN | 0.43 | rs1157900390 |
| 150 | p.Ser150Thr | c.448T>A | 2.74e-06 | 4 / 0 | 3.74 | 0.560 | AMBIGUOUS | 0.68 | rs1164698280 |
| 150 | p.Ser150Tyr | c.449C>A | 6.84e-07 | 1 / 0 | 3.74 | - | nan | - | |
| 76 | p.Leu76Met | c.226C>A | 1.09e-05 | 16 / 0 | 3.54 | 0.902 | PATHOGENIC | 0.89 | rs1754337139 |
| 179 | p.Gly179Arg | c.535G>A | 3.42e-06 | 5 / 0 | 3.37 | 0.996 | PATHOGENIC | 0.62 | rs1338417647 |
| 179 | p.Gly179Glu | c.536G>A | 6.84e-07 | 1 / 0 | 3.37 | - | nan | - | rs761926773 |
| 96 | p.Phe96Leu | c.286T>C | 6.84e-07 | 1 / 0 | 3.33 | 0.457 | AMBIGUOUS | 0.41 | rs1218611866 |
| 60 | p.Ile60Val | c.178A>G | 2.74e-06 | 4 / 0 | 3.33 | 0.061 | BENIGN | 0.78 | |
| 60 | p.Ile60Thr | c.179T>C | 6.84e-07 | 1 / 0 | 3.33 | - | nan | - | |
| 96 | p.Phe96Ser | c.287T>C | 5.47e-06 | 8 / 0 | 3.33 | - | nan | - | rs750200746 |
| 77 | p.Arg77Trp | c.229C>T | 9.51e-05 | 139 / 0 | 3.24 | 0.517 | AMBIGUOUS | 0.73 | rs571519472 |
| 82 | p.Tyr82Phe | c.245A>T | 6.84e-07 | 1 / 0 | 3.24 | 0.144 | BENIGN | 0.71 | |
| 77 | p.Arg77Gln | c.230G>A | 1.37e-06 | 2 / 0 | 3.24 | - | nan | - | rs1754337326 |
| 82 | p.Tyr82Cys | c.245A>G | 2.74e-06 | 4 / 0 | 3.24 | - | nan | - | rs768227684 |
| 84 | p.Ile84Leu | c.250A>C | 1.37e-06 | 2 / 0 | 3.22 | 0.562 | AMBIGUOUS | 0.86 | |
| 84 | p.Ile84Thr | c.251T>C | 6.84e-07 | 1 / 0 | 3.22 | - | nan | - | |
| 176 | p.Ile176Val | c.526A>G | 1.98e-05 | 29 / 0 | 3.15 | 0.220 | BENIGN | 0.90 | rs759306305 |
Complete RRCS results
Top 1000 contact pairs by |ΔRRCS|. Significance threshold: |ΔRRCS| ≥ 2.28, computed as max(mean(|Δ|) + σ, 0.2). Highlighted rows indicate significant changes.
| Res1 | AA1 | Res2 | AA2 | Active RRCS | Inactive RRCS | ΔRRCS | |ΔRRCS| |
|---|---|---|---|---|---|---|---|
| 467 | ARG | 468 | HIS | 11.572 | 0.000 | 11.572 | 11.572 |
| 468 | HIS | 469 | ASP | 10.241 | 0.000 | 10.241 | 10.241 |
| 243 | LYS | 250 | GLU | 0.000 | 7.840 | -7.840 | 7.840 |
| 233 | THR | 289 | GLU | 7.771 | 0.000 | 7.771 | 7.771 |
| 115 | ARG | 192 | ASP | 1.800 | 9.341 | -7.541 | 7.541 |
| 148 | ARG | 149 | TYR | 4.241 | 11.182 | -6.941 | 6.941 |
| 80 | THR | 143 | ARG | 0.000 | 6.808 | -6.808 | 6.808 |
| 186 | GLU | 200 | GLU | 8.587 | 15.148 | -6.560 | 6.560 |
| 295 | THR | 351 | SER | 0.851 | 7.049 | -6.199 | 6.199 |
| 236 | ASN | 241 | VAL | 0.000 | 6.095 | -6.095 | 6.095 |
| 506 | GLN | 507 | PRO | 0.000 | 6.063 | -6.063 | 6.063 |
| 91 | ASP | 341 | SER | 9.193 | 3.188 | 6.006 | 6.006 |
| 238 | GLU | 286 | PHE | 5.960 | 0.000 | 5.960 | 5.960 |
| 286 | PHE | 290 | LYS | 5.894 | 0.000 | 5.894 | 5.894 |
| 142 | ASP | 143 | ARG | 0.000 | 5.117 | -5.117 | 5.117 |
| 234 | THR | 289 | GLU | 5.062 | 0.000 | 5.062 | 5.062 |
| 303 | PHE | 340 | ASN | 0.000 | 4.920 | -4.920 | 4.920 |
| 340 | ASN | 344 | ASN | 4.844 | 0.000 | 4.844 | 4.844 |
| 307 | TRP | 337 | GLY | 3.821 | 8.646 | -4.825 | 4.825 |
| 361 | ARG | 366 | GLN | 0.000 | 4.778 | -4.778 | 4.778 |
| 73 | ASN | 75 | HIS | 4.704 | 0.000 | 4.704 | 4.704 |
| 240 | GLY | 255 | ILE | 0.000 | 4.703 | -4.703 | 4.703 |
| 244 | GLU | 278 | SER | 4.694 | 0.000 | 4.694 | 4.694 |
| 310 | PHE | 314 | LEU | 1.001 | 5.542 | -4.540 | 4.540 |
| 133 | ILE | 307 | TRP | 4.522 | 0.000 | 4.522 | 4.522 |
| 303 | PHE | 307 | TRP | 2.469 | 6.920 | -4.451 | 4.451 |
| 349 | PRO | 355 | PHE | 5.114 | 0.708 | 4.406 | 4.406 |
| 143 | ARG | 223 | TYR | 4.328 | 0.000 | 4.328 | 4.328 |
| 250 | GLU | 254 | ARG | 4.305 | 0.000 | 4.305 | 4.305 |
| 223 | TYR | 296 | LEU | 2.317 | 6.549 | -4.233 | 4.233 |
| 144 | TYR | 148 | ARG | 2.052 | 6.262 | -4.211 | 4.211 |
| 186 | GLU | 199 | GLU | 0.000 | 4.208 | -4.208 | 4.208 |
| 133 | ILE | 303 | PHE | 1.379 | 5.454 | -4.074 | 4.074 |
| 244 | GLU | 282 | LYS | 6.410 | 2.337 | 4.074 | 4.074 |
| 349 | PRO | 356 | LYS | 0.465 | 4.489 | -4.024 | 4.024 |
| 136 | LEU | 348 | TYR | 3.958 | 0.000 | 3.958 | 3.958 |
| 267 | SER | 268 | THR | 0.000 | 3.787 | -3.787 | 3.787 |
| 237 | LEU | 285 | LYS | 3.784 | 0.000 | 3.784 | 3.784 |
| 150 | SER | 286 | PHE | 0.000 | 3.742 | -3.742 | 3.742 |
| 144 | TYR | 222 | MET | 4.215 | 0.546 | 3.669 | 3.669 |
| 75 | HIS | 76 | LEU | 3.536 | 0.000 | 3.536 | 3.536 |
| 179 | GLY | 184 | TRP | 5.755 | 2.384 | 3.371 | 3.371 |
| 60 | ILE | 96 | PHE | 0.559 | 3.888 | -3.328 | 3.328 |
| 348 | TYR | 355 | PHE | 0.000 | 3.314 | -3.314 | 3.314 |
| 77 | ARG | 82 | TYR | 3.341 | 0.102 | 3.239 | 3.239 |
| 84 | ILE | 348 | TYR | 0.262 | 3.482 | -3.220 | 3.220 |
| 176 | ILE | 203 | TYR | 3.146 | 0.000 | 3.146 | 3.146 |
| 402 | SER | 403 | ARG | 0.347 | 3.311 | -2.964 | 2.964 |
| 409 | ASP | 410 | SER | 2.958 | 0.000 | 2.958 | 2.958 |
| 223 | TYR | 297 | GLY | 0.000 | 2.884 | -2.884 | 2.884 |
| 307 | TRP | 340 | ASN | 6.198 | 3.343 | 2.855 | 2.855 |
| 180 | PRO | 185 | LYS | 2.933 | 0.110 | 2.824 | 2.824 |
| 2 | ASN | 3 | PRO | 0.084 | 2.903 | -2.819 | 2.819 |
| 404 | LYS | 405 | ASP | 0.000 | 2.802 | -2.802 | 2.802 |
| 115 | ARG | 185 | LYS | 2.789 | 0.000 | 2.789 | 2.789 |
| 352 | SER | 354 | GLU | 3.474 | 0.717 | 2.757 | 2.757 |
| 223 | TYR | 300 | VAL | 2.088 | 4.812 | -2.724 | 2.724 |
| 105 | LEU | 110 | TYR | 3.593 | 0.870 | 2.723 | 2.723 |
| 285 | LYS | 289 | GLU | 2.794 | 0.092 | 2.703 | 2.703 |
| 115 | ARG | 188 | ALA | 2.933 | 0.232 | 2.701 | 2.701 |
| 75 | HIS | 361 | ARG | 2.694 | 0.000 | 2.694 | 2.694 |
| 133 | ILE | 212 | PHE | 0.122 | 2.813 | -2.691 | 2.691 |
| 484 | SER | 485 | PRO | 0.511 | 3.164 | -2.653 | 2.653 |
| 63 | ASN | 91 | ASP | 4.624 | 1.976 | 2.649 | 2.649 |
| 180 | PRO | 198 | THR | 2.604 | 0.000 | 2.604 | 2.604 |
| 40 | LEU | 44 | ARG | 2.596 | 0.000 | 2.596 | 2.596 |
| 115 | ARG | 119 | ASP | 2.582 | 0.000 | 2.582 | 2.582 |
| 55 | PHE | 339 | PHE | 1.421 | 3.996 | -2.575 | 2.575 |
| 186 | GLU | 198 | THR | 0.000 | 2.546 | -2.546 | 2.546 |
| 55 | PHE | 338 | TYR | 0.000 | 2.517 | -2.517 | 2.517 |
| 136 | LEU | 303 | PHE | 1.490 | 3.994 | -2.505 | 2.505 |
| 354 | GLU | 357 | ARG | 0.000 | 2.478 | -2.478 | 2.478 |
| 63 | ASN | 88 | ALA | 5.506 | 3.038 | 2.469 | 2.469 |
| 151 | LEU | 244 | GLU | 0.000 | 2.465 | -2.465 | 2.465 |
| 191 | ASP | 193 | LYS | 2.462 | 0.000 | 2.462 | 2.462 |
| 81 | ASN | 355 | PHE | 3.322 | 0.948 | 2.374 | 2.374 |
| 234 | THR | 286 | PHE | 2.374 | 0.000 | 2.374 | 2.374 |
| 143 | ARG | 296 | LEU | 0.000 | 2.352 | -2.352 | 2.352 |
| 425 | SER | 426 | PRO | 2.726 | 0.390 | 2.336 | 2.336 |
| 417 | SER | 418 | GLN | 2.334 | 0.000 | 2.334 | 2.334 |
| 55 | PHE | 342 | CYS | 1.041 | 3.372 | -2.330 | 2.330 |
| 237 | LEU | 283 | LEU | 0.000 | 2.290 | -2.290 | 2.290 |
| 58 | PHE | 342 | CYS | 1.891 | 4.167 | -2.277 | 2.277 |
| 219 | ILE | 303 | PHE | 2.254 | 0.000 | 2.254 | 2.254 |
| 150 | SER | 289 | GLU | 0.000 | 2.208 | -2.208 | 2.208 |
| 228 | ILE | 232 | ARG | 2.190 | 0.000 | 2.190 | 2.190 |
| 91 | ASP | 344 | ASN | 4.744 | 2.581 | 2.163 | 2.163 |
| 242 | MET | 286 | PHE | 0.000 | 2.144 | -2.144 | 2.144 |
| 212 | PHE | 311 | PHE | 14.751 | 16.853 | -2.102 | 2.102 |
| 234 | THR | 290 | LYS | 0.123 | 2.184 | -2.062 | 2.062 |
| 244 | GLU | 279 | ILE | 2.058 | 0.000 | 2.058 | 2.058 |
| 515 | LEU | 516 | ALA | 0.000 | 2.031 | -2.031 | 2.031 |
| 496 | CYS | 497 | GLU | 2.029 | 0.000 | 2.029 | 2.029 |
| 143 | ARG | 348 | TYR | 2.025 | 0.000 | 2.025 | 2.025 |
| 362 | ILE | 367 | CYS | 0.000 | 1.996 | -1.996 | 1.996 |
| 511 | SER | 512 | ASN | 0.000 | 1.982 | -1.982 | 1.982 |
| 3 | PRO | 4 | ASP | 0.000 | 1.978 | -1.978 | 1.978 |
| 34 | ASN | 36 | THR | 1.937 | 0.000 | 1.937 | 1.937 |
| 143 | ARG | 153 | TYR | 0.000 | 1.935 | -1.935 | 1.935 |
| 151 | LEU | 282 | LYS | 0.000 | 1.913 | -1.913 | 1.913 |
| 234 | THR | 238 | GLU | 0.000 | 1.832 | -1.832 | 1.832 |
| 199 | GLU | 318 | SER | 1.251 | 3.077 | -1.825 | 1.825 |
| 87 | LEU | 136 | LEU | 2.592 | 0.772 | 1.820 | 1.820 |
| 143 | ARG | 351 | SER | 1.818 | 0.000 | 1.818 | 1.818 |
| 472 | PRO | 473 | LEU | 1.816 | 0.000 | 1.816 | 1.816 |
| 67 | ILE | 89 | MET | 2.575 | 0.782 | 1.793 | 1.793 |
| 421 | LEU | 422 | PRO | 2.269 | 0.479 | 1.790 | 1.790 |
| 233 | THR | 286 | PHE | 0.000 | 1.787 | -1.787 | 1.787 |
| 66 | VAL | 348 | TYR | 0.000 | 1.774 | -1.774 | 1.774 |
| 144 | TYR | 225 | ARG | 5.529 | 3.758 | 1.771 | 1.771 |
| 75 | HIS | 354 | GLU | 0.000 | 1.769 | -1.769 | 1.769 |
| 6 | ASP | 7 | THR | 0.000 | 1.765 | -1.765 | 1.765 |
| 349 | PRO | 359 | PHE | 1.689 | 3.452 | -1.763 | 1.763 |
| 41 | ASP | 44 | ARG | 0.000 | 1.757 | -1.757 | 1.757 |
| 137 | CYS | 215 | PRO | 1.280 | 3.016 | -1.736 | 1.736 |
| 51 | VAL | 55 | PHE | 1.736 | 0.000 | 1.736 | 1.736 |
| 501 | ASP | 502 | VAL | 0.793 | 2.525 | -1.732 | 1.732 |
| 306 | CYS | 340 | ASN | 2.800 | 1.087 | 1.713 | 1.713 |
| 413 | CYS | 414 | LEU | 1.699 | 0.000 | 1.699 | 1.699 |
| 82 | TYR | 159 | ARG | 1.536 | 3.227 | -1.691 | 1.691 |
| 185 | LYS | 198 | THR | 2.400 | 4.063 | -1.662 | 1.662 |
| 216 | LEU | 303 | PHE | 1.654 | 0.000 | 1.654 | 1.654 |
| 219 | ILE | 300 | VAL | 0.093 | 1.736 | -1.643 | 1.643 |
| 62 | GLY | 345 | PRO | 3.208 | 4.815 | -1.608 | 1.608 |
| 241 | VAL | 282 | LYS | 1.607 | 0.000 | 1.607 | 1.607 |
| 106 | GLU | 335 | TRP | 2.592 | 1.019 | 1.573 | 1.573 |
| 9 | HIS | 10 | ASN | 3.851 | 2.283 | 1.568 | 1.568 |
| 4 | ASP | 5 | LEU | 0.000 | 1.565 | -1.565 | 1.565 |
| 397 | LEU | 398 | GLU | 3.395 | 1.844 | 1.551 | 1.551 |
| 223 | TYR | 299 | VAL | 1.548 | 0.000 | 1.548 | 1.548 |
| 66 | VAL | 349 | PRO | 1.539 | 0.000 | 1.539 | 1.539 |
| 339 | PHE | 343 | LEU | 4.413 | 2.878 | 1.535 | 1.535 |
| 237 | LEU | 282 | LYS | 1.522 | 0.000 | 1.522 | 1.522 |
| 299 | VAL | 348 | TYR | 1.894 | 0.374 | 1.519 | 1.519 |
| 55 | PHE | 335 | TRP | 0.000 | 1.519 | -1.519 | 1.519 |
| 408 | ASP | 409 | ASP | 1.755 | 0.270 | 1.486 | 1.486 |
| 203 | TYR | 207 | SER | 1.587 | 3.057 | -1.470 | 1.470 |
| 146 | GLY | 289 | GLU | 0.000 | 1.459 | -1.459 | 1.459 |
| 69 | SER | 355 | PHE | 0.783 | 2.240 | -1.457 | 1.457 |
| 500 | ALA | 501 | ASP | 1.455 | 0.000 | 1.455 | 1.455 |
| 262 | GLU | 263 | ASP | 0.000 | 1.446 | -1.446 | 1.446 |
| 419 | ARG | 420 | THR | 1.441 | 0.000 | 1.441 | 1.441 |
| 481 | GLU | 482 | PRO | 2.027 | 0.592 | 1.435 | 1.435 |
| 40 | LEU | 107 | VAL | 0.000 | 1.421 | -1.421 | 1.421 |
| 310 | PHE | 311 | PHE | 1.922 | 0.510 | 1.412 | 1.412 |
| 69 | SER | 359 | PHE | 3.421 | 2.015 | 1.406 | 1.406 |
| 140 | SER | 222 | MET | 2.910 | 1.513 | 1.397 | 1.397 |
| 63 | ASN | 345 | PRO | 4.845 | 3.451 | 1.393 | 1.393 |
| 454 | LEU | 455 | SER | 1.387 | 0.000 | 1.387 | 1.387 |
| 198 | THR | 203 | TYR | 1.748 | 3.131 | -1.383 | 1.383 |
| 87 | LEU | 135 | SER | 2.638 | 1.257 | 1.381 | 1.381 |
| 41 | ASP | 43 | THR | 0.000 | 1.374 | -1.374 | 1.374 |
| 185 | LYS | 200 | GLU | 1.373 | 0.000 | 1.373 | 1.373 |
| 139 | ILE | 348 | TYR | 1.367 | 0.000 | 1.367 | 1.367 |
| 255 | ILE | 259 | ASN | 1.351 | 0.000 | 1.351 | 1.351 |
| 86 | ASN | 89 | MET | 0.000 | 1.338 | -1.338 | 1.338 |
| 461 | PRO | 462 | PRO | 2.407 | 1.070 | 1.337 | 1.337 |
| 80 | THR | 142 | ASP | 5.744 | 4.424 | 1.321 | 1.321 |
| 93 | LEU | 97 | THR | 0.000 | 1.313 | -1.313 | 1.313 |
| 361 | ARG | 367 | CYS | 0.000 | 1.308 | -1.308 | 1.308 |
| 344 | ASN | 348 | TYR | 1.708 | 3.012 | -1.304 | 1.304 |
| 335 | TRP | 338 | TYR | 2.092 | 0.803 | 1.289 | 1.289 |
| 137 | CYS | 219 | ILE | 2.359 | 3.646 | -1.287 | 1.287 |
| 492 | SER | 493 | ASN | 2.095 | 3.378 | -1.283 | 1.283 |
| 295 | THR | 296 | LEU | 0.000 | 1.279 | -1.279 | 1.279 |
| 156 | LEU | 161 | LYS | 3.117 | 1.843 | 1.274 | 1.274 |
| 320 | PHE | 323 | LEU | 2.121 | 0.848 | 1.273 | 1.273 |
| 93 | LEU | 128 | CYS | 1.042 | 2.311 | -1.270 | 1.270 |
| 63 | ASN | 341 | SER | 1.651 | 2.920 | -1.269 | 1.269 |
| 176 | ILE | 210 | GLY | 0.156 | 1.421 | -1.265 | 1.265 |
| 412 | SER | 413 | CYS | 0.021 | 1.276 | -1.254 | 1.254 |
| 426 | PRO | 427 | SER | 1.253 | 0.000 | 1.253 | 1.253 |
| 139 | ILE | 143 | ARG | 1.448 | 0.196 | 1.252 | 1.252 |
| 176 | ILE | 207 | SER | 1.248 | 0.000 | 1.248 | 1.248 |
| 133 | ILE | 311 | PHE | 1.247 | 0.000 | 1.247 | 1.247 |
| 278 | SER | 282 | LYS | 1.242 | 0.000 | 1.242 | 1.242 |
| 240 | GLY | 282 | LYS | 1.241 | 0.000 | 1.241 | 1.241 |
| 469 | ASP | 470 | SER | 1.226 | 0.000 | 1.226 | 1.226 |
| 456 | LEU | 457 | PRO | 0.026 | 1.252 | -1.226 | 1.226 |
| 86 | ASN | 170 | TRP | 5.394 | 4.171 | 1.222 | 1.222 |
| 222 | MET | 225 | ARG | 0.000 | 1.222 | -1.222 | 1.222 |
| 102 | SER | 338 | TYR | 3.505 | 2.292 | 1.213 | 1.213 |
| 347 | ILE | 348 | TYR | 1.201 | 0.000 | 1.201 | 1.201 |
| 250 | GLU | 252 | THR | 0.000 | 1.200 | -1.200 | 1.200 |
| 184 | TRP | 203 | TYR | 3.238 | 2.040 | 1.198 | 1.198 |
| 199 | GLU | 324 | LYS | 2.525 | 3.721 | -1.197 | 1.197 |
| 510 | LYS | 511 | SER | 1.189 | 0.000 | 1.189 | 1.189 |
| 344 | ASN | 349 | PRO | 1.259 | 0.071 | 1.188 | 1.188 |
| 306 | CYS | 336 | LEU | 6.314 | 7.496 | -1.182 | 1.182 |
| 65 | LEU | 359 | PHE | 4.622 | 3.445 | 1.177 | 1.177 |
| 240 | GLY | 253 | LEU | 0.000 | 1.170 | -1.170 | 1.170 |
| 137 | CYS | 218 | VAL | 1.558 | 0.389 | 1.169 | 1.169 |
| 90 | ALA | 132 | SER | 0.929 | 2.096 | -1.167 | 1.167 |
| 106 | GLU | 331 | LYS | 7.067 | 8.228 | -1.161 | 1.161 |
| 281 | VAL | 285 | LYS | 0.000 | 1.147 | -1.147 | 1.147 |
| 52 | LEU | 335 | TRP | 5.169 | 6.305 | -1.136 | 1.136 |
| 263 | ASP | 264 | THR | 0.000 | 1.127 | -1.127 | 1.127 |
| 302 | MET | 343 | LEU | 0.612 | 1.737 | -1.125 | 1.125 |
| 111 | TRP | 121 | TRP | 4.563 | 3.444 | 1.120 | 1.120 |
| 73 | ASN | 358 | ALA | 1.190 | 2.301 | -1.111 | 1.111 |
| 184 | TRP | 200 | GLU | 1.141 | 0.033 | 1.109 | 1.109 |
| 213 | TYR | 311 | PHE | 2.890 | 3.990 | -1.100 | 1.100 |
| 60 | ILE | 92 | LEU | 1.138 | 2.225 | -1.087 | 1.087 |
| 64 | ILE | 92 | LEU | 0.971 | 2.057 | -1.086 | 1.086 |
| 212 | PHE | 213 | TYR | 1.727 | 0.645 | 1.083 | 1.083 |
| 334 | PHE | 338 | TYR | 7.343 | 8.425 | -1.082 | 1.082 |
| 37 | LEU | 38 | PRO | 0.758 | 1.838 | -1.080 | 1.080 |
| 98 | VAL | 338 | TYR | 0.693 | 1.766 | -1.073 | 1.073 |
| 115 | ARG | 189 | PRO | 1.349 | 0.283 | 1.067 | 1.067 |
| 516 | ALA | 517 | PRO | 0.707 | 1.774 | -1.066 | 1.066 |
| 127 | LEU | 170 | TRP | 1.190 | 0.128 | 1.063 | 1.063 |
| 430 | TYR | 431 | LEU | 1.058 | 0.000 | 1.058 | 1.058 |
| 226 | VAL | 296 | LEU | 1.047 | 0.000 | 1.047 | 1.047 |
| 424 | ALA | 425 | SER | 1.044 | 0.000 | 1.044 | 1.044 |
| 70 | VAL | 81 | ASN | 0.008 | 1.043 | -1.035 | 1.035 |
| 149 | TYR | 152 | GLN | 1.691 | 0.665 | 1.026 | 1.026 |
| 147 | VAL | 230 | ALA | 0.000 | 1.020 | -1.020 | 1.020 |
| 10 | ASN | 11 | THR | 1.016 | 0.000 | 1.016 | 1.016 |
| 237 | LEU | 286 | PHE | 1.154 | 0.147 | 1.007 | 1.007 |
| 110 | TYR | 112 | VAL | 1.033 | 2.035 | -1.002 | 1.002 |
| 133 | ILE | 219 | ILE | 0.086 | 1.085 | -1.000 | 1.000 |
| 227 | TYR | 293 | ALA | 0.993 | 0.000 | 0.993 | 0.993 |
| 141 | ILE | 218 | VAL | 1.007 | 0.022 | 0.985 | 0.985 |
| 423 | SER | 424 | ALA | 0.976 | 0.000 | 0.976 | 0.976 |
| 139 | ILE | 296 | LEU | 0.000 | 0.972 | -0.972 | 0.972 |
| 70 | VAL | 355 | PHE | 1.821 | 0.851 | 0.970 | 0.970 |
| 94 | LEU | 99 | LEU | 4.178 | 3.210 | 0.968 | 0.968 |
| 274 | ASN | 275 | PRO | 1.146 | 0.181 | 0.965 | 0.965 |
| 152 | GLN | 156 | LEU | 0.962 | 0.000 | 0.962 | 0.962 |
| 216 | LEU | 304 | ILE | 0.628 | 1.584 | -0.957 | 0.957 |
| 45 | ALA | 107 | VAL | 2.868 | 1.918 | 0.950 | 0.950 |
| 487 | THR | 488 | ASP | 0.000 | 0.938 | -0.938 | 0.938 |
| 209 | LEU | 214 | ILE | 4.563 | 5.498 | -0.935 | 0.935 |
| 68 | LEU | 362 | ILE | 1.178 | 2.107 | -0.929 | 0.929 |
| 353 | LYS | 354 | GLU | 0.914 | 0.000 | 0.914 | 0.914 |
| 230 | ALA | 289 | GLU | 0.912 | 0.000 | 0.912 | 0.912 |
| 460 | GLU | 461 | PRO | 3.426 | 2.526 | 0.900 | 0.900 |
| 40 | LEU | 45 | ALA | 0.000 | 0.895 | -0.895 | 0.895 |
| 127 | LEU | 177 | SER | 3.162 | 4.048 | -0.886 | 0.886 |
| 87 | LEU | 91 | ASP | 1.445 | 0.564 | 0.880 | 0.880 |
| 83 | PHE | 142 | ASP | 2.607 | 1.732 | 0.876 | 0.876 |
| 11 | THR | 12 | SER | 0.869 | 0.000 | 0.869 | 0.869 |
| 76 | LEU | 81 | ASN | 5.731 | 6.598 | -0.867 | 0.867 |
| 133 | ILE | 215 | PRO | 1.069 | 1.896 | -0.827 | 0.827 |
| 237 | LEU | 289 | GLU | 0.814 | 0.000 | 0.814 | 0.814 |
| 148 | ARG | 229 | VAL | 0.086 | 0.897 | -0.811 | 0.811 |
| 71 | ALA | 77 | ARG | 2.736 | 3.547 | -0.811 | 0.811 |
| 307 | TRP | 338 | TYR | 0.000 | 0.804 | -0.804 | 0.804 |
| 464 | ARG | 465 | ARG | 0.794 | 0.000 | 0.794 | 0.794 |
| 232 | ARG | 236 | ASN | 0.000 | 0.790 | -0.790 | 0.790 |
| 299 | VAL | 347 | ILE | 1.092 | 1.881 | -0.789 | 0.789 |
| 72 | CYS | 367 | CYS | 0.000 | 0.786 | -0.786 | 0.786 |
| 21 | LYS | 22 | ASN | 0.000 | 0.783 | -0.783 | 0.783 |
| 121 | TRP | 334 | PHE | 0.017 | 0.795 | -0.778 | 0.778 |
| 149 | TYR | 156 | LEU | 1.218 | 0.445 | 0.774 | 0.774 |
| 60 | ILE | 95 | SER | 2.954 | 2.185 | 0.768 | 0.768 |
| 291 | LYS | 351 | SER | 0.000 | 0.765 | -0.765 | 0.765 |
| 140 | SER | 223 | TYR | 3.347 | 4.109 | -0.762 | 0.762 |
| 87 | LEU | 132 | SER | 2.821 | 3.582 | -0.760 | 0.760 |
| 221 | VAL | 225 | ARG | 1.276 | 0.518 | 0.758 | 0.758 |
| 132 | SER | 344 | ASN | 0.757 | 0.000 | 0.757 | 0.757 |
| 177 | SER | 178 | ILE | 0.757 | 0.000 | 0.757 | 0.757 |
| 66 | VAL | 355 | PHE | 0.756 | 0.000 | 0.756 | 0.756 |
| 69 | SER | 362 | ILE | 2.735 | 1.981 | 0.754 | 0.754 |
| 76 | LEU | 355 | PHE | 0.043 | 0.795 | -0.752 | 0.752 |
| 184 | TRP | 198 | THR | 0.752 | 0.000 | 0.752 | 0.752 |
| 99 | LEU | 338 | TYR | 4.717 | 3.971 | 0.746 | 0.746 |
| 102 | SER | 335 | TRP | 1.942 | 1.203 | 0.740 | 0.740 |
| 76 | LEU | 354 | GLU | 1.550 | 0.813 | 0.737 | 0.737 |
| 208 | SER | 311 | PHE | 1.978 | 1.251 | 0.727 | 0.727 |
| 144 | TYR | 226 | VAL | 2.203 | 2.930 | -0.726 | 0.726 |
| 59 | ALA | 95 | SER | 2.325 | 1.603 | 0.723 | 0.723 |
| 90 | ALA | 170 | TRP | 1.893 | 1.180 | 0.713 | 0.713 |
| 111 | TRP | 195 | CYS | 5.802 | 6.511 | -0.709 | 0.709 |
| 126 | VAL | 177 | SER | 2.147 | 1.439 | 0.709 | 0.709 |
| 201 | PRO | 318 | SER | 3.182 | 3.886 | -0.704 | 0.704 |
| 306 | CYS | 337 | GLY | 2.027 | 2.729 | -0.702 | 0.702 |
| 472 | PRO | 474 | PHE | 0.000 | 0.701 | -0.701 | 0.701 |
| 205 | LEU | 209 | LEU | 0.571 | 1.268 | -0.697 | 0.697 |
| 200 | GLU | 318 | SER | 0.000 | 0.696 | -0.696 | 0.696 |
| 86 | ASN | 166 | LEU | 2.616 | 3.311 | -0.695 | 0.695 |
| 307 | TRP | 310 | PHE | 0.000 | 0.694 | -0.694 | 0.694 |
| 345 | PRO | 359 | PHE | 0.089 | 0.782 | -0.692 | 0.692 |
| 110 | TYR | 193 | LYS | 1.778 | 2.469 | -0.691 | 0.691 |
| 253 | LEU | 279 | ILE | 0.000 | 0.687 | -0.687 | 0.687 |
| 97 | THR | 124 | VAL | 0.758 | 1.439 | -0.681 | 0.681 |
| 111 | TRP | 192 | ASP | 1.818 | 1.141 | 0.677 | 0.677 |
| 84 | ILE | 139 | ILE | 0.852 | 0.182 | 0.669 | 0.669 |
| 237 | LEU | 242 | MET | 0.000 | 0.667 | -0.667 | 0.667 |
| 502 | VAL | 503 | ALA | 0.664 | 0.000 | 0.664 | 0.664 |
| 94 | LEU | 341 | SER | 1.544 | 0.882 | 0.662 | 0.662 |
| 310 | PHE | 330 | PHE | 0.380 | 1.039 | -0.659 | 0.659 |
| 314 | LEU | 330 | PHE | 0.265 | 0.921 | -0.656 | 0.656 |
| 63 | ASN | 344 | ASN | 0.000 | 0.654 | -0.654 | 0.654 |
| 185 | LYS | 196 | GLY | 0.000 | 0.652 | -0.652 | 0.652 |
| 134 | LEU | 172 | LEU | 1.704 | 2.356 | -0.652 | 0.652 |
| 102 | SER | 334 | PHE | 0.000 | 0.650 | -0.650 | 0.650 |
| 322 | THR | 323 | LEU | 0.327 | 0.976 | -0.649 | 0.649 |
| 159 | ARG | 163 | ILE | 0.252 | 0.900 | -0.648 | 0.648 |
| 304 | ILE | 308 | LEU | 1.436 | 0.790 | 0.646 | 0.646 |
| 55 | PHE | 99 | LEU | 0.000 | 0.646 | -0.646 | 0.646 |
| 73 | ASN | 361 | ARG | 3.582 | 2.940 | 0.642 | 0.642 |
| 145 | ILE | 153 | TYR | 1.385 | 0.755 | 0.630 | 0.630 |
| 140 | SER | 218 | VAL | 0.621 | 0.000 | 0.621 | 0.621 |
| 220 | LEU | 300 | VAL | 0.621 | 0.000 | 0.621 | 0.621 |
| 357 | ARG | 361 | ARG | 0.618 | 0.000 | 0.618 | 0.618 |
| 74 | ARG | 446 | GLU | 0.615 | 0.000 | 0.615 | 0.615 |
| 15 | ALA | 16 | HIS | 0.000 | 0.614 | -0.614 | 0.614 |
| 118 | CYS | 195 | CYS | 6.613 | 6.000 | 0.613 | 0.613 |
| 2 | ASN | 4 | ASP | 0.611 | 0.000 | 0.611 | 0.611 |
| 286 | PHE | 289 | GLU | 0.000 | 0.611 | -0.611 | 0.611 |
| 5 | LEU | 6 | ASP | 0.000 | 0.606 | -0.606 | 0.606 |
| 136 | LEU | 299 | VAL | 0.000 | 0.603 | -0.603 | 0.603 |
| 197 | VAL | 314 | LEU | 0.000 | 0.603 | -0.603 | 0.603 |
| 210 | GLY | 215 | PRO | 1.693 | 2.285 | -0.593 | 0.593 |
| 304 | ILE | 309 | PRO | 0.954 | 0.364 | 0.589 | 0.589 |
| 227 | TYR | 231 | LYS | 0.915 | 1.503 | -0.588 | 0.588 |
| 358 | ALA | 361 | ARG | 0.582 | 0.000 | 0.582 | 0.582 |
| 87 | LEU | 139 | ILE | 0.758 | 0.185 | 0.573 | 0.573 |
| 265 | LEU | 266 | SER | 0.614 | 0.049 | 0.565 | 0.565 |
| 132 | SER | 340 | ASN | 0.561 | 0.000 | 0.561 | 0.561 |
| 101 | PHE | 121 | TRP | 1.575 | 1.014 | 0.561 | 0.561 |
| 221 | VAL | 222 | MET | 0.007 | 0.560 | -0.553 | 0.553 |
| 403 | ARG | 404 | LYS | 0.000 | 0.551 | -0.551 | 0.551 |
| 140 | SER | 219 | ILE | 2.838 | 2.287 | 0.551 | 0.551 |
| 223 | TYR | 348 | TYR | 0.550 | 0.000 | 0.550 | 0.550 |
| 233 | THR | 237 | LEU | 0.187 | 0.732 | -0.546 | 0.546 |
| 79 | PRO | 157 | VAL | 0.452 | 0.997 | -0.546 | 0.546 |
| 87 | LEU | 348 | TYR | 1.332 | 0.790 | 0.542 | 0.542 |
| 125 | ASP | 334 | PHE | 1.075 | 0.534 | 0.541 | 0.541 |
| 86 | ASN | 135 | SER | 2.650 | 2.110 | 0.541 | 0.541 |
| 69 | SER | 70 | VAL | 0.000 | 0.541 | -0.541 | 0.541 |
| 74 | ARG | 77 | ARG | 0.539 | 0.000 | 0.539 | 0.539 |
| 317 | GLY | 324 | LYS | 0.714 | 0.182 | 0.532 | 0.532 |
| 189 | PRO | 194 | GLU | 0.543 | 0.014 | 0.529 | 0.529 |
| 119 | ASP | 185 | LYS | 2.196 | 1.668 | 0.528 | 0.528 |
| 178 | ILE | 182 | LEU | 1.373 | 1.901 | -0.528 | 0.528 |
| 112 | VAL | 113 | LEU | 0.000 | 0.528 | -0.528 | 0.528 |
| 105 | LEU | 195 | CYS | 0.473 | 1.001 | -0.528 | 0.528 |
| 44 | ARG | 106 | GLU | 0.000 | 0.527 | -0.527 | 0.527 |
| 105 | LEU | 111 | TRP | 2.435 | 1.912 | 0.523 | 0.523 |
| 399 | ARG | 400 | SER | 3.000 | 2.478 | 0.523 | 0.523 |
| 94 | LEU | 129 | CYS | 1.036 | 0.515 | 0.522 | 0.522 |
| 212 | PHE | 307 | TRP | 3.325 | 2.804 | 0.521 | 0.521 |
| 66 | VAL | 359 | PHE | 2.898 | 2.378 | 0.519 | 0.519 |
| 291 | LYS | 353 | LYS | 0.000 | 0.519 | -0.519 | 0.519 |
| 262 | GLU | 264 | THR | 0.517 | 0.000 | 0.517 | 0.517 |
| 200 | GLU | 201 | PRO | 1.321 | 1.839 | -0.517 | 0.517 |
| 20 | LEU | 21 | LYS | 0.514 | 0.000 | 0.514 | 0.514 |
| 121 | TRP | 125 | ASP | 0.511 | 0.000 | 0.511 | 0.511 |
| 95 | SER | 100 | PRO | 1.701 | 2.210 | -0.509 | 0.509 |
| 491 | ALA | 492 | SER | 0.195 | 0.702 | -0.507 | 0.507 |
| 212 | PHE | 308 | LEU | 4.117 | 3.612 | 0.506 | 0.506 |
| 407 | LEU | 408 | ASP | 0.771 | 0.266 | 0.505 | 0.505 |
| 310 | PHE | 333 | VAL | 3.442 | 3.947 | -0.505 | 0.505 |
| 73 | ASN | 76 | LEU | 1.877 | 1.376 | 0.500 | 0.500 |
| 172 | LEU | 176 | ILE | 0.418 | 0.914 | -0.496 | 0.496 |
| 96 | PHE | 97 | THR | 0.671 | 0.177 | 0.493 | 0.493 |
| 247 | ASN | 248 | SER | 0.000 | 0.492 | -0.492 | 0.492 |
| 499 | ALA | 500 | ALA | 0.488 | 0.000 | 0.488 | 0.488 |
| 274 | ASN | 277 | SER | 0.486 | 0.000 | 0.486 | 0.486 |
| 65 | LEU | 362 | ILE | 0.703 | 1.184 | -0.480 | 0.480 |
| 212 | PHE | 303 | PHE | 1.692 | 1.213 | 0.479 | 0.479 |
| 14 | PRO | 15 | ALA | 0.556 | 0.078 | 0.478 | 0.478 |
| 123 | ALA | 181 | LEU | 0.284 | 0.761 | -0.477 | 0.477 |
| 147 | VAL | 229 | VAL | 1.492 | 1.965 | -0.473 | 0.473 |
| 147 | VAL | 226 | VAL | 0.236 | 0.708 | -0.472 | 0.472 |
| 81 | ASN | 84 | ILE | 0.350 | 0.820 | -0.470 | 0.470 |
| 405 | ASP | 406 | SER | 0.000 | 0.466 | -0.466 | 0.466 |
| 278 | SER | 279 | ILE | 0.000 | 0.462 | -0.462 | 0.462 |
| 302 | MET | 347 | ILE | 1.115 | 0.657 | 0.458 | 0.458 |
| 155 | THR | 161 | LYS | 0.458 | 0.000 | 0.458 | 0.458 |
| 56 | ILE | 99 | LEU | 3.538 | 3.081 | 0.456 | 0.456 |
| 305 | LEU | 336 | LEU | 0.433 | 0.886 | -0.453 | 0.453 |
| 285 | LYS | 288 | ARG | 0.000 | 0.453 | -0.453 | 0.453 |
| 91 | ASP | 132 | SER | 3.012 | 2.559 | 0.453 | 0.453 |
| 136 | LEU | 344 | ASN | 0.453 | 0.000 | 0.453 | 0.453 |
| 266 | SER | 267 | SER | 0.000 | 0.451 | -0.451 | 0.451 |
| 90 | ALA | 128 | CYS | 1.522 | 1.073 | 0.449 | 0.449 |
| 131 | ALA | 170 | TRP | 1.666 | 1.217 | 0.448 | 0.448 |
| 67 | ILE | 85 | VAL | 0.068 | 0.514 | -0.446 | 0.446 |
| 13 | ALA | 14 | PRO | 0.284 | 0.730 | -0.446 | 0.446 |
| 145 | ILE | 156 | LEU | 0.684 | 1.130 | -0.445 | 0.445 |
| 257 | SER | 259 | ASN | 0.000 | 0.440 | -0.440 | 0.440 |
| 136 | LEU | 340 | ASN | 0.436 | 0.000 | 0.436 | 0.436 |
| 82 | TYR | 166 | LEU | 1.149 | 0.714 | 0.435 | 0.435 |
| 204 | ALA | 318 | SER | 0.734 | 0.299 | 0.435 | 0.435 |
| 40 | LEU | 108 | LEU | 0.000 | 0.434 | -0.434 | 0.434 |
| 218 | VAL | 222 | MET | 0.675 | 0.243 | 0.432 | 0.432 |
| 279 | ILE | 283 | LEU | 0.899 | 0.468 | 0.432 | 0.432 |
| 414 | LEU | 415 | SER | 0.565 | 0.142 | 0.423 | 0.423 |
| 134 | LEU | 169 | VAL | 3.424 | 3.004 | 0.420 | 0.420 |
| 299 | VAL | 344 | ASN | 0.000 | 0.417 | -0.417 | 0.417 |
| 352 | SER | 355 | PHE | 2.874 | 2.460 | 0.414 | 0.414 |
| 241 | VAL | 253 | LEU | 0.000 | 0.406 | -0.406 | 0.406 |
| 418 | GLN | 419 | ARG | 0.402 | 0.000 | 0.402 | 0.402 |
| 398 | GLU | 399 | ARG | 1.458 | 1.858 | -0.400 | 0.400 |
| 98 | VAL | 121 | TRP | 4.926 | 4.528 | 0.397 | 0.397 |
| 186 | GLU | 187 | PRO | 1.226 | 0.830 | 0.396 | 0.396 |
| 92 | LEU | 96 | PHE | 0.000 | 0.396 | -0.396 | 0.396 |
| 115 | ARG | 190 | ASN | 0.000 | 0.395 | -0.395 | 0.395 |
| 209 | LEU | 215 | PRO | 0.223 | 0.616 | -0.393 | 0.393 |
| 130 | THR | 211 | SER | 1.254 | 1.645 | -0.392 | 0.392 |
| 204 | ALA | 314 | LEU | 0.392 | 0.000 | 0.392 | 0.392 |
| 310 | PHE | 334 | PHE | 1.870 | 2.261 | -0.391 | 0.391 |
| 177 | SER | 203 | TYR | 0.380 | 0.000 | 0.380 | 0.380 |
| 213 | TYR | 312 | ILE | 0.878 | 1.258 | -0.379 | 0.379 |
| 56 | ILE | 95 | SER | 1.014 | 0.637 | 0.377 | 0.377 |
| 435 | ALA | 436 | PRO | 0.000 | 0.376 | -0.376 | 0.376 |
| 90 | ALA | 131 | ALA | 0.954 | 0.581 | 0.373 | 0.373 |
| 44 | ARG | 48 | VAL | 0.000 | 0.372 | -0.372 | 0.372 |
| 174 | THR | 178 | ILE | 1.056 | 0.685 | 0.371 | 0.371 |
| 227 | TYR | 296 | LEU | 0.370 | 0.000 | 0.370 | 0.370 |
| 129 | CYS | 338 | TYR | 0.368 | 0.000 | 0.368 | 0.368 |
| 140 | SER | 141 | ILE | 0.367 | 0.000 | 0.367 | 0.367 |
| 226 | VAL | 293 | ALA | 0.000 | 0.364 | -0.364 | 0.364 |
| 57 | LEU | 61 | VAL | 0.444 | 0.807 | -0.363 | 0.363 |
| 94 | LEU | 98 | VAL | 1.667 | 2.029 | -0.362 | 0.362 |
| 136 | LEU | 219 | ILE | 1.389 | 1.750 | -0.360 | 0.360 |
| 178 | ILE | 181 | LEU | 0.357 | 0.000 | 0.357 | 0.357 |
| 325 | PRO | 330 | PHE | 0.399 | 0.043 | 0.356 | 0.356 |
| 231 | LYS | 234 | THR | 0.353 | 0.000 | 0.353 | 0.353 |
| 125 | ASP | 338 | TYR | 6.480 | 6.128 | 0.353 | 0.353 |
| 79 | PRO | 162 | ALA | 0.349 | 0.000 | 0.349 | 0.349 |
| 236 | ASN | 242 | MET | 0.000 | 0.343 | -0.343 | 0.343 |
| 302 | MET | 305 | LEU | 0.697 | 0.356 | 0.341 | 0.341 |
| 49 | GLY | 107 | VAL | 1.343 | 1.004 | 0.339 | 0.339 |
| 295 | THR | 347 | ILE | 0.000 | 0.338 | -0.338 | 0.338 |
| 229 | VAL | 233 | THR | 0.000 | 0.336 | -0.336 | 0.336 |
| 63 | ASN | 95 | SER | 0.336 | 0.000 | 0.336 | 0.336 |
| 284 | PHE | 353 | LYS | 0.335 | 0.000 | 0.335 | 0.335 |
| 313 | ALA | 333 | VAL | 0.358 | 0.024 | 0.334 | 0.334 |
| 56 | ILE | 100 | PRO | 1.362 | 1.029 | 0.333 | 0.333 |
| 143 | ARG | 226 | VAL | 0.749 | 1.079 | -0.331 | 0.331 |
| 274 | ASN | 276 | ARG | 0.328 | 0.000 | 0.328 | 0.328 |
| 220 | LEU | 304 | ILE | 0.223 | 0.550 | -0.326 | 0.326 |
| 473 | LEU | 475 | THR | 0.000 | 0.322 | -0.322 | 0.322 |
| 143 | ARG | 299 | VAL | 0.322 | 0.000 | 0.322 | 0.322 |
| 140 | SER | 226 | VAL | 0.000 | 0.319 | -0.319 | 0.319 |
| 144 | TYR | 229 | VAL | 0.000 | 0.318 | -0.318 | 0.318 |
| 137 | CYS | 141 | ILE | 0.318 | 0.000 | 0.318 | 0.318 |
| 95 | SER | 99 | LEU | 0.752 | 1.069 | -0.317 | 0.317 |
| 98 | VAL | 125 | ASP | 3.903 | 3.589 | 0.314 | 0.314 |
| 111 | TRP | 117 | PHE | 2.129 | 2.439 | -0.311 | 0.311 |
| 251 | LEU | 255 | ILE | 0.309 | 0.000 | 0.309 | 0.309 |
| 55 | PHE | 56 | ILE | 0.000 | 0.309 | -0.309 | 0.309 |
| 98 | VAL | 124 | VAL | 1.661 | 1.353 | 0.308 | 0.308 |
| 513 | MET | 514 | PRO | 0.712 | 1.019 | -0.307 | 0.307 |
| 354 | GLU | 361 | ARG | 0.302 | 0.000 | 0.302 | 0.302 |
| 111 | TRP | 113 | LEU | 1.508 | 1.808 | -0.300 | 0.300 |
| 42 | ILE | 46 | ILE | 0.700 | 0.996 | -0.296 | 0.296 |
| 24 | ASN | 26 | THR | 0.296 | 0.000 | 0.296 | 0.296 |
| 176 | ILE | 211 | SER | 0.575 | 0.281 | 0.295 | 0.295 |
| 264 | THR | 265 | LEU | 0.737 | 0.444 | 0.292 | 0.292 |
| 133 | ILE | 216 | LEU | 0.000 | 0.292 | -0.292 | 0.292 |
| 308 | LEU | 312 | ILE | 2.440 | 2.150 | 0.291 | 0.291 |
| 48 | VAL | 107 | VAL | 0.097 | 0.385 | -0.288 | 0.288 |
| 460 | GLU | 462 | PRO | 0.000 | 0.284 | -0.284 | 0.284 |
| 309 | PRO | 333 | VAL | 2.967 | 3.251 | -0.284 | 0.284 |
| 242 | MET | 251 | LEU | 0.000 | 0.283 | -0.283 | 0.283 |
| 179 | GLY | 203 | TYR | 0.559 | 0.841 | -0.282 | 0.282 |
| 392 | THR | 393 | ARG | 0.282 | 0.000 | 0.282 | 0.282 |
| 127 | LEU | 173 | SER | 3.892 | 3.611 | 0.281 | 0.281 |
| 30 | GLN | 31 | THR | 0.000 | 0.279 | -0.279 | 0.279 |
| 48 | VAL | 106 | GLU | 0.042 | 0.320 | -0.278 | 0.278 |
| 147 | VAL | 286 | PHE | 0.000 | 0.277 | -0.277 | 0.277 |
| 212 | PHE | 216 | LEU | 1.777 | 1.503 | 0.274 | 0.274 |
| 79 | PRO | 153 | TYR | 0.168 | 0.441 | -0.273 | 0.273 |
| 130 | THR | 173 | SER | 5.202 | 4.931 | 0.271 | 0.271 |
| 329 | VAL | 333 | VAL | 0.043 | 0.313 | -0.270 | 0.270 |
| 353 | LYS | 357 | ARG | 0.267 | 0.000 | 0.267 | 0.267 |
| 130 | THR | 176 | ILE | 0.753 | 1.018 | -0.265 | 0.265 |
| 153 | TYR | 157 | VAL | 4.714 | 4.451 | 0.263 | 0.263 |
| 84 | ILE | 355 | PHE | 0.880 | 0.617 | 0.263 | 0.263 |
| 70 | VAL | 76 | LEU | 2.021 | 1.758 | 0.263 | 0.263 |
| 458 | ALA | 459 | PRO | 1.039 | 0.782 | 0.257 | 0.257 |
| 126 | VAL | 203 | TYR | 0.289 | 0.033 | 0.256 | 0.256 |
| 141 | ILE | 222 | MET | 0.926 | 1.182 | -0.256 | 0.256 |
| 185 | LYS | 188 | ALA | 0.253 | 0.000 | 0.253 | 0.253 |
| 316 | LEU | 323 | LEU | 0.251 | 0.000 | 0.251 | 0.251 |
| 31 | THR | 32 | SER | 0.251 | 0.000 | 0.251 | 0.251 |
| 309 | PRO | 336 | LEU | 0.272 | 0.520 | -0.248 | 0.248 |
| 307 | TRP | 311 | PHE | 2.488 | 2.735 | -0.246 | 0.246 |
| 198 | THR | 204 | ALA | 0.000 | 0.245 | -0.245 | 0.245 |
| 332 | VAL | 336 | LEU | 0.244 | 0.000 | 0.244 | 0.244 |
| 66 | VAL | 88 | ALA | 1.070 | 0.827 | 0.243 | 0.243 |
| 468 | HIS | 470 | SER | 0.000 | 0.243 | -0.243 | 0.243 |
| 118 | CYS | 196 | GLY | 0.408 | 0.165 | 0.243 | 0.243 |
| 69 | SER | 76 | LEU | 1.433 | 1.675 | -0.242 | 0.242 |
| 108 | LEU | 112 | VAL | 0.734 | 0.976 | -0.242 | 0.242 |
| 452 | ALA | 453 | LEU | 0.240 | 0.000 | 0.240 | 0.240 |
| 314 | LEU | 315 | PRO | 1.330 | 1.092 | 0.238 | 0.238 |
| 97 | THR | 121 | TRP | 0.054 | 0.292 | -0.238 | 0.238 |
| 298 | ILE | 351 | SER | 0.000 | 0.237 | -0.237 | 0.237 |
| 318 | SER | 319 | LEU | 0.237 | 0.000 | 0.237 | 0.237 |
| 300 | VAL | 304 | ILE | 0.809 | 0.573 | 0.236 | 0.236 |
| 207 | SER | 211 | SER | 0.308 | 0.072 | 0.236 | 0.236 |
| 61 | VAL | 65 | LEU | 0.617 | 0.383 | 0.234 | 0.234 |
| 243 | LYS | 247 | ASN | 0.232 | 0.000 | 0.232 | 0.232 |
| 59 | ALA | 341 | SER | 0.703 | 0.474 | 0.229 | 0.229 |
| 126 | VAL | 207 | SER | 0.000 | 0.227 | -0.227 | 0.227 |
| 94 | LEU | 125 | ASP | 1.439 | 1.212 | 0.227 | 0.227 |
| 141 | ILE | 145 | ILE | 0.879 | 0.654 | 0.225 | 0.225 |
| 509 | PHE | 510 | LYS | 0.839 | 1.060 | -0.221 | 0.221 |
| 83 | PHE | 138 | ALA | 0.987 | 1.208 | -0.221 | 0.221 |
| 273 | HIS | 278 | SER | 0.219 | 0.000 | 0.219 | 0.219 |
| 287 | SER | 291 | LYS | 0.218 | 0.000 | 0.218 | 0.218 |
| 121 | TRP | 122 | ALA | 0.772 | 0.554 | 0.218 | 0.218 |
| 121 | TRP | 195 | CYS | 1.499 | 1.283 | 0.216 | 0.216 |
| 93 | LEU | 98 | VAL | 0.446 | 0.230 | 0.216 | 0.216 |
| 241 | VAL | 283 | LEU | 0.214 | 0.000 | 0.214 | 0.214 |
| 111 | TRP | 118 | CYS | 6.157 | 5.944 | 0.213 | 0.213 |
| 298 | ILE | 347 | ILE | 0.000 | 0.212 | -0.212 | 0.212 |
| 59 | ALA | 92 | LEU | 0.000 | 0.210 | -0.210 | 0.210 |
| 163 | ILE | 167 | LEU | 0.565 | 0.358 | 0.208 | 0.208 |
| 295 | THR | 299 | VAL | 0.205 | 0.000 | 0.205 | 0.205 |
| 306 | CYS | 343 | LEU | 0.595 | 0.800 | -0.205 | 0.205 |
| 60 | ILE | 64 | ILE | 0.887 | 0.683 | 0.204 | 0.204 |
| 130 | THR | 134 | LEU | 0.759 | 0.963 | -0.204 | 0.204 |
| 231 | LYS | 235 | LYS | 0.377 | 0.578 | -0.202 | 0.202 |
| 101 | PHE | 111 | TRP | 0.946 | 1.148 | -0.201 | 0.201 |
| 58 | PHE | 346 | ILE | 1.759 | 1.959 | -0.200 | 0.200 |
| 48 | VAL | 52 | LEU | 0.950 | 1.148 | -0.199 | 0.199 |
| 180 | PRO | 203 | TYR | 5.877 | 5.679 | 0.198 | 0.198 |
| 223 | TYR | 293 | ALA | 0.000 | 0.195 | -0.195 | 0.195 |
| 401 | GLN | 402 | SER | 0.097 | 0.291 | -0.195 | 0.195 |
| 145 | ILE | 150 | SER | 0.392 | 0.198 | 0.194 | 0.194 |
| 101 | PHE | 113 | LEU | 2.182 | 1.990 | 0.192 | 0.192 |
| 185 | LYS | 186 | GLU | 0.000 | 0.192 | -0.192 | 0.192 |
| 144 | TYR | 145 | ILE | 0.189 | 0.000 | 0.189 | 0.189 |
| 129 | CYS | 307 | TRP | 2.893 | 2.707 | 0.186 | 0.186 |
| 142 | ASP | 157 | VAL | 1.075 | 1.261 | -0.186 | 0.186 |
| 396 | SER | 397 | LEU | 1.333 | 1.519 | -0.186 | 0.186 |
| 78 | THR | 81 | ASN | 3.943 | 3.757 | 0.186 | 0.186 |
| 87 | LEU | 344 | ASN | 2.908 | 2.722 | 0.186 | 0.186 |
| 108 | LEU | 110 | TYR | 0.822 | 0.637 | 0.185 | 0.185 |
| 205 | LEU | 315 | PRO | 0.184 | 0.000 | 0.184 | 0.184 |
| 312 | ILE | 316 | LEU | 0.874 | 1.057 | -0.183 | 0.183 |
| 121 | TRP | 338 | TYR | 0.554 | 0.372 | 0.182 | 0.182 |
| 437 | PRO | 438 | PRO | 0.633 | 0.815 | -0.182 | 0.182 |
| 64 | ILE | 68 | LEU | 1.122 | 0.940 | 0.182 | 0.182 |
| 184 | TRP | 202 | PHE | 0.542 | 0.723 | -0.181 | 0.181 |
| 310 | PHE | 315 | PRO | 0.181 | 0.000 | 0.181 | 0.181 |
| 114 | GLY | 192 | ASP | 0.236 | 0.055 | 0.181 | 0.181 |
| 219 | ILE | 223 | TYR | 1.292 | 1.111 | 0.181 | 0.181 |
| 273 | HIS | 277 | SER | 0.180 | 0.000 | 0.180 | 0.180 |
| 66 | VAL | 344 | ASN | 0.000 | 0.180 | -0.180 | 0.180 |
| 317 | GLY | 323 | LEU | 0.177 | 0.000 | 0.177 | 0.177 |
| 436 | PRO | 437 | PRO | 0.928 | 0.751 | 0.177 | 0.177 |
| 459 | PRO | 460 | GLU | 0.000 | 0.177 | -0.177 | 0.177 |
| 70 | VAL | 77 | ARG | 1.589 | 1.414 | 0.176 | 0.176 |
| 503 | ALA | 504 | ASN | 0.176 | 0.000 | 0.176 | 0.176 |
| 355 | PHE | 359 | PHE | 0.357 | 0.184 | 0.173 | 0.173 |
| 83 | PHE | 139 | ILE | 2.175 | 2.002 | 0.173 | 0.173 |
| 44 | ARG | 331 | LYS | 0.000 | 0.173 | -0.173 | 0.173 |
| 199 | GLU | 330 | PHE | 0.169 | 0.000 | 0.169 | 0.169 |
| 139 | ILE | 223 | TYR | 0.385 | 0.216 | 0.169 | 0.169 |
| 208 | SER | 315 | PRO | 0.862 | 0.694 | 0.167 | 0.167 |
| 498 | ALA | 499 | ALA | 0.167 | 0.000 | 0.167 | 0.167 |
| 211 | SER | 311 | PHE | 1.827 | 1.662 | 0.165 | 0.165 |
| 80 | THR | 153 | TYR | 2.556 | 2.392 | 0.164 | 0.164 |
| 444 | PHE | 445 | PRO | 0.392 | 0.556 | -0.164 | 0.164 |
| 188 | ALA | 189 | PRO | 0.808 | 0.647 | 0.161 | 0.161 |
| 152 | GLN | 155 | THR | 0.459 | 0.616 | -0.157 | 0.157 |
| 240 | GLY | 254 | ARG | 0.000 | 0.156 | -0.156 | 0.156 |
| 320 | PHE | 322 | THR | 0.376 | 0.220 | 0.156 | 0.156 |
| 303 | PHE | 308 | LEU | 0.205 | 0.051 | 0.154 | 0.154 |
| 135 | SER | 169 | VAL | 0.992 | 1.145 | -0.153 | 0.153 |
| 306 | CYS | 307 | TRP | 0.378 | 0.225 | 0.153 | 0.153 |
| 63 | ASN | 92 | LEU | 2.732 | 2.581 | 0.151 | 0.151 |
| 347 | ILE | 351 | SER | 0.000 | 0.151 | -0.151 | 0.151 |
| 238 | GLU | 290 | LYS | 0.149 | 0.000 | 0.149 | 0.149 |
| 118 | CYS | 194 | GLU | 0.149 | 0.000 | 0.149 | 0.149 |
| 131 | ALA | 169 | VAL | 1.247 | 1.395 | -0.148 | 0.148 |
| 427 | SER | 428 | PRO | 2.066 | 2.213 | -0.147 | 0.147 |
| 155 | THR | 156 | LEU | 0.147 | 0.000 | 0.147 | 0.147 |
| 69 | SER | 358 | ALA | 1.335 | 1.188 | 0.146 | 0.146 |
| 123 | ALA | 177 | SER | 2.867 | 2.723 | 0.144 | 0.144 |
| 200 | GLU | 203 | TYR | 0.572 | 0.430 | 0.142 | 0.142 |
| 339 | PHE | 342 | CYS | 0.544 | 0.403 | 0.142 | 0.142 |
| 86 | ASN | 169 | VAL | 0.334 | 0.193 | 0.141 | 0.141 |
| 142 | ASP | 153 | TYR | 6.086 | 6.225 | -0.139 | 0.139 |
| 99 | LEU | 335 | TRP | 0.075 | 0.213 | -0.138 | 0.138 |
| 126 | VAL | 176 | ILE | 0.493 | 0.356 | 0.136 | 0.136 |
| 111 | TRP | 193 | LYS | 5.427 | 5.292 | 0.135 | 0.135 |
| 115 | ARG | 191 | ASP | 0.135 | 0.000 | 0.135 | 0.135 |
| 143 | ARG | 292 | ALA | 0.000 | 0.133 | -0.133 | 0.133 |
| 344 | ASN | 345 | PRO | 0.891 | 1.023 | -0.133 | 0.133 |
| 67 | ILE | 92 | LEU | 0.744 | 0.875 | -0.131 | 0.131 |
| 52 | LEU | 103 | ALA | 1.532 | 1.661 | -0.129 | 0.129 |
| 400 | SER | 401 | GLN | 4.415 | 4.286 | 0.129 | 0.129 |
| 93 | LEU | 170 | TRP | 0.944 | 0.820 | 0.124 | 0.124 |
| 47 | SER | 48 | VAL | 0.025 | 0.150 | -0.124 | 0.124 |
| 319 | LEU | 320 | PHE | 1.318 | 1.193 | 0.124 | 0.124 |
| 359 | PHE | 364 | GLY | 0.124 | 0.000 | 0.124 | 0.124 |
| 94 | LEU | 338 | TYR | 1.051 | 0.928 | 0.123 | 0.123 |
| 156 | LEU | 157 | VAL | 0.137 | 0.015 | 0.122 | 0.122 |
| 102 | SER | 121 | TRP | 3.936 | 4.057 | -0.121 | 0.121 |
| 131 | ALA | 173 | SER | 4.086 | 3.966 | 0.120 | 0.120 |
| 325 | PRO | 329 | VAL | 0.652 | 0.770 | -0.118 | 0.118 |
| 83 | PHE | 165 | ALA | 0.646 | 0.529 | 0.117 | 0.117 |
| 385 | ALA | 387 | THR | 0.116 | 0.000 | 0.116 | 0.116 |
| 316 | LEU | 320 | PHE | 0.490 | 0.382 | 0.108 | 0.108 |
| 343 | LEU | 347 | ILE | 0.450 | 0.557 | -0.106 | 0.106 |
| 103 | ALA | 335 | TRP | 2.206 | 2.312 | -0.106 | 0.106 |
| 205 | LEU | 208 | SER | 0.094 | 0.198 | -0.104 | 0.104 |
| 208 | SER | 213 | TYR | 6.082 | 6.185 | -0.103 | 0.103 |
| 158 | THR | 161 | LYS | 2.227 | 2.124 | 0.103 | 0.103 |
| 214 | ILE | 215 | PRO | 1.454 | 1.352 | 0.101 | 0.101 |
| 157 | VAL | 162 | ALA | 0.099 | 0.000 | 0.099 | 0.099 |
| 216 | LEU | 308 | LEU | 0.126 | 0.028 | 0.098 | 0.098 |
| 248 | SER | 252 | THR | 0.098 | 0.000 | 0.098 | 0.098 |
| 298 | ILE | 302 | MET | 0.337 | 0.240 | 0.097 | 0.097 |
| 113 | LEU | 117 | PHE | 1.371 | 1.275 | 0.096 | 0.096 |
| 62 | GLY | 359 | PHE | 0.175 | 0.080 | 0.096 | 0.096 |
| 174 | THR | 177 | SER | 0.198 | 0.103 | 0.096 | 0.096 |
| 134 | LEU | 215 | PRO | 0.606 | 0.702 | -0.095 | 0.095 |
| 158 | THR | 160 | ARG | 0.462 | 0.366 | 0.095 | 0.095 |
| 144 | TYR | 149 | TYR | 0.106 | 0.011 | 0.095 | 0.095 |
| 150 | SER | 151 | LEU | 0.186 | 0.281 | -0.095 | 0.095 |
| 330 | PHE | 333 | VAL | 0.094 | 0.000 | 0.094 | 0.094 |
| 59 | ALA | 99 | LEU | 0.000 | 0.093 | -0.093 | 0.093 |
| 303 | PHE | 304 | ILE | 0.127 | 0.035 | 0.092 | 0.092 |
| 94 | LEU | 128 | CYS | 2.867 | 2.956 | -0.090 | 0.090 |
| 169 | VAL | 173 | SER | 0.203 | 0.293 | -0.090 | 0.090 |
| 136 | LEU | 139 | ILE | 0.174 | 0.260 | -0.086 | 0.086 |
| 346 | ILE | 350 | CYS | 0.000 | 0.085 | -0.085 | 0.085 |
| 201 | PRO | 202 | PHE | 0.085 | 0.000 | 0.085 | 0.085 |
| 76 | LEU | 358 | ALA | 0.609 | 0.524 | 0.085 | 0.085 |
| 147 | VAL | 289 | GLU | 0.000 | 0.084 | -0.084 | 0.084 |
| 72 | CYS | 73 | ASN | 0.231 | 0.147 | 0.084 | 0.084 |
| 96 | PHE | 101 | PHE | 0.000 | 0.084 | -0.084 | 0.084 |
| 315 | PRO | 318 | SER | 0.083 | 0.000 | 0.083 | 0.083 |
| 244 | GLU | 251 | LEU | 0.000 | 0.083 | -0.083 | 0.083 |
| 59 | ALA | 342 | CYS | 1.646 | 1.564 | 0.083 | 0.083 |
| 308 | LEU | 309 | PRO | 0.848 | 0.929 | -0.082 | 0.082 |
| 207 | SER | 212 | PHE | 0.150 | 0.068 | 0.081 | 0.081 |
| 83 | PHE | 166 | LEU | 2.699 | 2.619 | 0.080 | 0.080 |
| 340 | ASN | 343 | LEU | 0.000 | 0.077 | -0.077 | 0.077 |
| 120 | ILE | 124 | VAL | 0.282 | 0.358 | -0.076 | 0.076 |
| 201 | PRO | 319 | LEU | 0.130 | 0.205 | -0.075 | 0.075 |
| 59 | ALA | 63 | ASN | 0.151 | 0.077 | 0.074 | 0.074 |
| 325 | PRO | 326 | PRO | 0.688 | 0.614 | 0.074 | 0.074 |
| 205 | LEU | 319 | LEU | 0.074 | 0.000 | 0.074 | 0.074 |
| 209 | LEU | 213 | TYR | 0.954 | 1.027 | -0.074 | 0.074 |
| 119 | ASP | 181 | LEU | 0.409 | 0.483 | -0.073 | 0.073 |
| 134 | LEU | 137 | CYS | 0.000 | 0.073 | -0.073 | 0.073 |
| 126 | VAL | 197 | VAL | 0.073 | 0.000 | 0.073 | 0.073 |
| 136 | LEU | 300 | VAL | 0.000 | 0.071 | -0.071 | 0.071 |
| 133 | ILE | 211 | SER | 2.716 | 2.645 | 0.071 | 0.071 |
| 359 | PHE | 363 | LEU | 0.068 | 0.000 | 0.068 | 0.068 |
| 389 | ARG | 390 | PRO | 0.323 | 0.390 | -0.066 | 0.066 |
| 235 | LYS | 238 | GLU | 0.145 | 0.080 | 0.066 | 0.066 |
| 74 | ARG | 444 | PHE | 0.000 | 0.065 | -0.065 | 0.065 |
| 315 | PRO | 319 | LEU | 0.000 | 0.064 | -0.064 | 0.064 |
| 165 | ALA | 168 | SER | 0.041 | 0.105 | -0.064 | 0.064 |
| 134 | LEU | 210 | GLY | 0.063 | 0.000 | 0.063 | 0.063 |
| 145 | ILE | 149 | TYR | 0.276 | 0.213 | 0.063 | 0.063 |
| 326 | PRO | 329 | VAL | 1.175 | 1.112 | 0.063 | 0.063 |
| 83 | PHE | 162 | ALA | 1.695 | 1.758 | -0.062 | 0.062 |
| 216 | LEU | 220 | LEU | 1.296 | 1.358 | -0.062 | 0.062 |
| 28 | PRO | 29 | ASN | 0.420 | 0.359 | 0.061 | 0.061 |
| 306 | CYS | 333 | VAL | 0.187 | 0.247 | -0.060 | 0.060 |
| 70 | VAL | 85 | VAL | 1.833 | 1.774 | 0.059 | 0.059 |
| 127 | LEU | 174 | THR | 2.515 | 2.458 | 0.057 | 0.057 |
| 449 | ALA | 450 | PRO | 0.559 | 0.505 | 0.054 | 0.054 |
| 140 | SER | 300 | VAL | 0.000 | 0.053 | -0.053 | 0.053 |
| 230 | ALA | 233 | THR | 0.053 | 0.000 | 0.053 | 0.053 |
| 56 | ILE | 60 | ILE | 0.799 | 0.851 | -0.053 | 0.053 |
| 171 | VAL | 175 | VAL | 0.057 | 0.005 | 0.051 | 0.051 |
| 78 | THR | 80 | THR | 0.324 | 0.375 | -0.051 | 0.051 |
| 63 | ASN | 66 | VAL | 0.000 | 0.050 | -0.050 | 0.050 |
| 66 | VAL | 345 | PRO | 0.996 | 1.044 | -0.049 | 0.049 |
| 98 | VAL | 102 | SER | 0.637 | 0.685 | -0.048 | 0.048 |
| 68 | LEU | 73 | ASN | 0.032 | 0.078 | -0.047 | 0.047 |
| 199 | GLU | 204 | ALA | 0.088 | 0.133 | -0.046 | 0.046 |
| 111 | TRP | 194 | GLU | 0.089 | 0.044 | 0.045 | 0.045 |
| 46 | ILE | 50 | LEU | 0.672 | 0.627 | 0.045 | 0.045 |
| 138 | ALA | 169 | VAL | 0.000 | 0.044 | -0.044 | 0.044 |
| 124 | VAL | 128 | CYS | 0.539 | 0.496 | 0.044 | 0.044 |
| 133 | ILE | 134 | LEU | 0.000 | 0.043 | -0.043 | 0.043 |
| 246 | SER | 247 | ASN | 0.041 | 0.000 | 0.041 | 0.041 |
| 67 | ILE | 88 | ALA | 1.118 | 1.078 | 0.040 | 0.040 |
| 65 | LEU | 363 | LEU | 0.482 | 0.442 | 0.040 | 0.040 |
| 361 | ARG | 370 | ARG | 0.000 | 0.040 | -0.040 | 0.040 |
| 203 | TYR | 208 | SER | 0.094 | 0.055 | 0.039 | 0.039 |
| 280 | ALA | 284 | PHE | 0.000 | 0.039 | -0.039 | 0.039 |
| 44 | ARG | 47 | SER | 0.039 | 0.000 | 0.039 | 0.039 |
| 57 | LEU | 62 | GLY | 0.000 | 0.039 | -0.039 | 0.039 |
| 59 | ALA | 345 | PRO | 0.038 | 0.000 | 0.038 | 0.038 |
| 348 | TYR | 349 | PRO | 0.859 | 0.822 | 0.038 | 0.038 |
| 116 | ILE | 120 | ILE | 0.454 | 0.418 | 0.037 | 0.037 |
| 241 | VAL | 252 | THR | 0.000 | 0.037 | -0.037 | 0.037 |
| 261 | HIS | 262 | GLU | 0.035 | 0.000 | 0.035 | 0.035 |
| 197 | VAL | 334 | PHE | 0.034 | 0.000 | 0.034 | 0.034 |
| 334 | PHE | 335 | TRP | 0.000 | 0.033 | -0.033 | 0.033 |
| 56 | ILE | 103 | ALA | 0.550 | 0.517 | 0.033 | 0.033 |
| 164 | LEU | 167 | LEU | 0.000 | 0.033 | -0.033 | 0.033 |
| 91 | ASP | 340 | ASN | 0.000 | 0.033 | -0.033 | 0.033 |
| 78 | THR | 79 | PRO | 0.971 | 0.938 | 0.033 | 0.033 |
| 95 | SER | 341 | SER | 0.353 | 0.385 | -0.032 | 0.032 |
| 197 | VAL | 198 | THR | 0.105 | 0.073 | 0.032 | 0.032 |
| 58 | PHE | 63 | ASN | 0.000 | 0.032 | -0.032 | 0.032 |
| 313 | ALA | 325 | PRO | 0.331 | 0.299 | 0.032 | 0.032 |
| 347 | ILE | 352 | SER | 0.000 | 0.031 | -0.031 | 0.031 |
| 104 | ALA | 109 | GLY | 0.109 | 0.081 | 0.028 | 0.028 |
| 317 | GLY | 325 | PRO | 0.027 | 0.000 | 0.027 | 0.027 |
| 126 | VAL | 130 | THR | 0.483 | 0.510 | -0.027 | 0.027 |
| 243 | LYS | 252 | THR | 0.000 | 0.025 | -0.025 | 0.025 |
| 99 | LEU | 100 | PRO | 0.740 | 0.764 | -0.025 | 0.025 |
| 52 | LEU | 106 | GLU | 0.523 | 0.546 | -0.023 | 0.023 |
| 115 | ARG | 118 | CYS | 0.023 | 0.000 | 0.023 | 0.023 |
| 145 | ILE | 157 | VAL | 0.678 | 0.656 | 0.023 | 0.023 |
| 134 | LEU | 173 | SER | 1.201 | 1.223 | -0.022 | 0.022 |
| 66 | VAL | 84 | ILE | 0.593 | 0.615 | -0.022 | 0.022 |
| 83 | PHE | 135 | SER | 0.021 | 0.000 | 0.021 | 0.021 |
| 146 | GLY | 153 | TYR | 3.003 | 3.023 | -0.020 | 0.020 |
| 44 | ARG | 49 | GLY | 0.000 | 0.020 | -0.020 | 0.020 |
| 306 | CYS | 339 | PHE | 0.041 | 0.021 | 0.019 | 0.019 |
| 80 | THR | 81 | ASN | 0.475 | 0.456 | 0.019 | 0.019 |
| 137 | CYS | 140 | SER | 0.019 | 0.000 | 0.019 | 0.019 |
| 153 | TYR | 154 | PRO | 1.312 | 1.329 | -0.017 | 0.017 |
| 302 | MET | 340 | ASN | 0.005 | 0.020 | -0.015 | 0.015 |
| 241 | VAL | 279 | ILE | 0.013 | 0.000 | 0.013 | 0.013 |
| 68 | LEU | 72 | CYS | 0.021 | 0.009 | 0.013 | 0.013 |
| 321 | SER | 322 | THR | 0.094 | 0.105 | -0.010 | 0.010 |
| 97 | THR | 98 | VAL | 0.640 | 0.630 | 0.010 | 0.010 |
| 85 | VAL | 89 | MET | 0.380 | 0.370 | 0.010 | 0.010 |
| 249 | LYS | 251 | LEU | 0.000 | 0.010 | -0.010 | 0.010 |
| 125 | ASP | 129 | CYS | 1.382 | 1.373 | 0.009 | 0.009 |
| 213 | TYR | 315 | PRO | 0.349 | 0.357 | -0.008 | 0.008 |
| 282 | LYS | 286 | PHE | 0.000 | 0.007 | -0.007 | 0.007 |
| 324 | LYS | 325 | PRO | 0.942 | 0.935 | 0.007 | 0.007 |
| 453 | LEU | 455 | SER | 0.000 | 0.007 | -0.007 | 0.007 |
| 182 | LEU | 184 | TRP | 0.000 | 0.006 | -0.006 | 0.006 |
| 104 | ALA | 108 | LEU | 0.006 | 0.000 | 0.006 | 0.006 |
| 211 | SER | 212 | PHE | 0.295 | 0.301 | -0.005 | 0.005 |
| 356 | LYS | 360 | VAL | 0.000 | 0.005 | -0.005 | 0.005 |
| 97 | THR | 128 | CYS | 0.000 | 0.005 | -0.005 | 0.005 |
| 294 | LYS | 298 | ILE | 0.336 | 0.331 | 0.004 | 0.004 |
| 152 | GLN | 246 | SER | 0.000 | 0.003 | -0.003 | 0.003 |
| 204 | ALA | 315 | PRO | 0.003 | 0.000 | 0.003 | 0.003 |
| 343 | LEU | 346 | ILE | 0.023 | 0.021 | 0.002 | 0.002 |
| 94 | LEU | 100 | PRO | 0.030 | 0.031 | -0.001 | 0.001 |
| 315 | PRO | 320 | PHE | 0.000 | 0.001 | -0.001 | 0.001 |
RRCS change distribution
0.01
Mean ΔRRCS
1.64
Std Dev
0.03
Median
Magnitude classification
16
High (|Δ| ≥ 5.0)
66
Medium (2.3 ≤ |Δ| < 5.0)
663
Low (|Δ| < 2.3)
Methods
RRCS analysis. Residue-Residue Contact Scores (RRCS) were calculated for each conformational state based on inter-atomic distances. Changes (ΔRRCS) were computed by comparing active and inactive structures predicted by AlphaFold multistate (del Alamo et al., 2022).
Significance threshold. |ΔRRCS| ≥ 2.28, computed as max(mean(|Δ|) + σ, 0.2). The 0.2 floor ensures receptors with very small overall change still surface their largest contacts.
Variant data. Population frequencies from gnomAD v4; pathogenicity predictions from AlphaMissense; conservation from ProtVar / UniProt.
Structural annotation. TM domain boundaries and generic numbering from GPCRdb.
What is RRCS?
Residue-Residue Contact Score (RRCS) measures how strongly two amino acids interact in a protein structure. When a protein changes shape (from inactive to active), these contact strengths change.
ΔRRCS (Delta RRCS) shows the difference in contact strength between states:
- Positive ΔRRCS. Contact is stronger in the active state.
- Negative ΔRRCS. Contact is stronger in the inactive state.
- Large |ΔRRCS|. Significant structural rearrangement.
Residues with large RRCS changes are critical for protein function. Mutations at these positions may disrupt the protein's ability to change shape properly.
Pathogenicity predictions
AlphaMissense predicts whether a mutation harms protein function:
- Pathogenic (score ≥ 0.564): mutation likely damages function.
- Ambiguous (0.34–0.563): effect uncertain.
- Benign (< 0.34): mutation likely tolerated.
Conservation & population data
Conservation score. How well-preserved a position is across species (0 = variable, 1 = conserved). High conservation suggests the position is critical for function.
Allele frequency. How often a variant appears in the population. Rare variants (low frequency) may be more likely to be harmful.
Sources: AlphaFold multistate · RRCS · gnomAD v4 · AlphaMissense · GPCRdb · UniProt / ProtVar