TSHR

Gene TSHR Glycoprotein hormone receptors Protein receptors UniProt P16473

UniProt ↗ GPCRdb ↗ NCBI Gene ↗

1864

Total Contact Pairs

201

Significant Changes

11.80

Max Increase

-12.89

Max Decrease

Interactive snake plot

GPCRdb-style topology. Click the view buttons to recolor residues by different data layers. Toggle contact links to draw significant residue-residue contacts as arcs. Click loop labels (ICL/ECL) to expand or collapse loop regions.

Color convention: blue = active-favoring, red = inactive-favoring.

Top 100 conformational changes

Residue pairs with the largest RRCS changes between active and inactive states. GPCRdb numbering in parentheses. Highlighted rows exceed the significance threshold.

Rank	Residue 1	Residue 2	Active RRCS	Inactive RRCS	ΔRRCS	Magnitude
1	GLU404	SER657 (7.32x31)	0.000	12.886	-12.886	HIGH
2	TYR601 (5.58x58)	MET626 (6.37x37)	1.202	13.307	-12.105	HIGH
3	GLU34	ARG38	11.795	0.000	+11.795	HIGH
4	TYR605 (5.62x62)	ARG609 (5.66x66)	0.965	11.908	-10.943	HIGH
5	TYR601 (5.58x58)	ILE630 (6.41x41)	2.110	11.552	-9.442	HIGH
6	PHE676 (7.51x51)	PHE681 (7.56x56)	8.328	0.000	+8.328	HIGH
7	GLU409	SER657 (7.32x31)	8.299	0.000	+8.299	HIGH
8	GLU404	VAL656 (7.31x30)	1.595	9.760	-8.165	HIGH
9	PHE405	VAL656 (7.31x30)	1.418	9.274	-7.856	HIGH
10	ASP617 (6.28x28)	LYS621 (6.32x32)	0.000	7.561	-7.561	HIGH
11	LYS262	ASN483	7.530	0.000	+7.530	HIGH
12	LEU732	HIS733	7.495	0.000	+7.495	HIGH
13	ARG625 (6.36x36)	PHE681 (7.56x56)	0.000	7.466	-7.466	HIGH
14	ARG112	ASN135	0.583	8.017	-7.435	HIGH
15	GLU107	ARG109	4.744	12.018	-7.274	HIGH
16	TYR643 (6.54x54)	SER659 (7.34x33)	0.000	7.146	-7.146	HIGH
17	PHE405	LYS565	6.911	0.000	+6.911	HIGH
18	LYS290	GLU394	6.845	0.000	+6.845	HIGH
19	MET735	GLU736	6.987	0.394	+6.593	HIGH
20	HIS347	ASN348	6.467	0.000	+6.467	HIGH
21	SER266	GLN289	0.000	6.423	-6.423	HIGH
22	GLU339	LYS340	0.000	6.220	-6.220	HIGH
23	TYR739	GLU740	6.179	0.000	+6.179	HIGH
24	SER561	ASP573	7.404	1.234	+6.169	HIGH
25	GLU30	LYS42	5.867	0.000	+5.867	HIGH
26	PRO675 (7.50x50)	ILE680 (7.55x55)	5.867	0.000	+5.867	HIGH
27	TYR444	ARG687 (8.52x52)	16.999	11.242	+5.757	HIGH
28	ASN372	GLN374	5.708	0.000	+5.708	HIGH
29	GLU328	ASN329	5.719	0.110	+5.608	HIGH
30	LEU263	ASN483	5.567	0.000	+5.567	HIGH
31	ARG519 (3.50x50)	TYR678 (7.53x53)	5.527	0.000	+5.527	HIGH
32	HIS105	PHE130	10.471	4.958	+5.513	HIGH
33	ARG293	CYS390	5.461	0.000	+5.461	HIGH
34	GLU740	LEU741	5.288	0.000	+5.288	HIGH
35	LYS262	HIS484	5.242	0.000	+5.242	HIGH
36	LEU439 (1.57x57)	ARG450 (2.40x40)	5.967	0.853	+5.114	HIGH
37	PHE153	THR179	0.058	5.148	-5.090	HIGH
38	PHE585 (5.42x42)	LEU589 (5.46x46)	0.255	5.342	-5.087	HIGH
39	CYS636 (6.47x47)	ASN670 (7.45x45)	7.240	2.185	+5.055	HIGH
40	ARG729	GLN730	5.073	0.085	+4.988	MED
41	LYS683 (8.48x48)	THR762	4.871	0.000	+4.871	MED
42	ASP410	HIS478	1.248	6.102	-4.855	MED
43	CYS408	TYR482	0.000	4.822	-4.822	MED
44	GLN704	GLN709	0.000	4.785	-4.785	MED
45	ILE411	LYS660 (7.35x34)	5.708	1.072	+4.636	MED
46	LYS291	GLU394	4.595	0.000	+4.595	MED
47	PHE423 (1.41x41)	LEU427 (1.45x45)	0.000	4.552	-4.552	MED
48	GLU251	ARG274	5.602	10.150	-4.548	MED
49	GLU404	LYS660 (7.35x34)	0.000	4.543	-4.543	MED
50	PHE405	LYS660 (7.35x34)	0.000	4.522	-4.522	MED
51	PHE594 (5.51x51)	PHE634 (6.45x45)	5.555	9.960	-4.406	MED
52	CYS301	CYS390	4.356	0.000	+4.356	MED
53	GLN33	PHE37	0.823	5.161	-4.338	MED
54	THR273	LYS291	4.322	0.000	+4.322	MED
55	ASN288	TYR482	0.000	4.258	-4.258	MED
56	HIS105	GLU107	4.200	0.000	+4.200	MED
57	ASP219	LYS244	5.281	1.087	+4.194	MED
58	SER243	SER268	0.000	4.170	-4.170	MED
59	TYR643 (6.54x54)	ILE654	3.023	7.191	-4.167	MED
60	GLU409	LYS660 (7.35x34)	4.153	0.000	+4.153	MED
61	TYR482	ASN483	4.052	8.175	-4.123	MED
62	PHE405	SER567	0.032	4.140	-4.108	MED
63	LYS261	ILE486	0.139	4.219	-4.081	MED
64	TYR444	ARG450 (2.40x40)	0.000	4.067	-4.067	MED
65	LEU429 (1.47x47)	GLY464 (2.54x54)	4.064	0.000	+4.064	MED
66	ASP403	PHE405	4.063	0.000	+4.063	MED
67	ASN734	MET735	4.037	0.000	+4.037	MED
68	GLN722	LYS723	4.009	0.000	+4.009	MED
69	SER304	ASP386	3.980	0.000	+3.980	MED
70	TYR82	HIS105	3.976	0.024	+3.952	MED
71	LYS261	THR490	0.000	3.932	-3.932	MED
72	GLN45	HIS63	7.128	3.212	+3.916	MED
73	PRO615 (6.26x26)	ASP619 (6.30x30)	4.041	0.154	+3.887	MED
74	ILE654	SER659 (7.34x33)	4.212	0.337	+3.875	MED
75	TYR279	ILE486	5.575	1.760	+3.816	MED
76	PHE631 (6.42x42)	ILE635 (6.46x46)	3.789	0.000	+3.789	MED
77	LYS129	ASP151	4.016	7.788	-3.772	MED
78	LYS287	CYS408	3.760	0.000	+3.760	MED
79	SER646 (6.57x57)	LYS651	10.525	6.768	+3.757	MED
80	SER25	PRO51	3.704	0.000	+3.704	MED
81	LEU267	PHE269	3.687	0.000	+3.687	MED
82	ASP530 (34.54x54)	ARG531 (34.55x55)	3.666	0.000	+3.666	MED
83	LEU693 (8.58x58)	ILE698	3.254	6.918	-3.664	MED
84	ASP403	ASN406	3.638	0.000	+3.638	MED
85	ARG293	GLU297	1.383	4.985	-3.603	MED
86	TYR643 (6.54x54)	LEU662 (7.37x36)	2.174	5.756	-3.582	MED
87	SER383	TYR385	0.000	3.544	-3.544	MED
88	PHE676 (7.51x51)	ILE680 (7.55x55)	1.454	4.998	-3.544	MED
89	GLU480	HIS484	3.843	0.302	+3.542	MED
90	THR159	TYR185	8.203	11.712	-3.509	MED
91	TRP488	CYS494 (3.25x25)	5.773	9.280	-3.507	MED
92	SER393	ASP395	3.495	0.000	+3.495	MED
93	GLU518 (3.49x49)	ARG519 (3.50x50)	0.141	3.632	-3.490	MED
94	ARG418 (1.36x36)	ASP474 (2.64x64)	9.502	6.023	+3.479	MED
95	GLN289	MET396	3.465	0.000	+3.465	MED
96	THR717	ASP718	3.457	0.000	+3.457	MED
97	TYR678 (7.53x53)	THR682 (8.47x47)	0.000	3.457	-3.457	MED
98	LYS291	ASP392	3.420	0.000	+3.420	MED
99	LYS291	ASP395	3.400	0.000	+3.400	MED
100	GLN704	VAL711	0.000	3.394	-3.394	MED

Transmembrane domain analysis

Active-favoring residues form stronger contacts in the active state (positive ΔRRCS). Inactive-favoring residues form stronger contacts in the inactive state (negative ΔRRCS). Only residues with |ΔRRCS| ≥ 2.22 are shown (per-receptor significance threshold = max(mean(|Δ|) + σ, 0.2)).

Segment	Range	Active-favoring residues	Count	Inactive-favoring residues	Count
TM1	418-439	439 (5.1), 429 (4.1), 418 (3.5)	3	423 (-4.6), 427 (-4.6)	2
TM2	449-474	450 (5.1), 464 (4.1), 474 (3.5), 460 (3.3), 449 (3.0)	5	453 (-3.1)	1
TM3	494-519	519 (5.5), 508 (3.3)	2	494 (-3.5), 518 (-3.5)	2
TM4	-	-	0	-	0
TM5	585-609	598 (3.2)	1	601 (-12.1), 605 (-10.9), 609 (-10.9), 585 (-5.1), 589 (-5.1), 594 (-4.4)	6
TM6	615-646	636 (5.1), 615 (3.9), 619 (3.9), 631 (3.8), 635 (3.8), 646 (3.8), 629 (3.3)	7	626 (-12.1), 630 (-9.4), 617 (-7.6), 621 (-7.6), 625 (-7.5), 643 (-7.1), 634 (-4.4)	7
TM7	656-681	676 (8.3), 681 (8.3), 675 (5.9), 680 (5.9), 678 (5.5), 670 (5.1), 660 (4.6)	7	657 (-12.9), 656 (-8.2), 659 (-7.1), 662 (-3.6), 666 (-3.3)	5
Intracellular / Extracellular loops & H8
ICL1	444-444	444 (5.8)	1	-	0
ICL2	530-531	530 (3.7), 531 (3.7)	2	-	0
ICL3	-	-	0	-	0
ECL1	478-490	483 (7.5), 484 (5.2), 480 (3.5)	3	478 (-4.9), 482 (-4.8), 486 (-4.1), 490 (-3.9), 488 (-3.5)	5
ECL2	561-573	565 (6.9), 561 (6.2), 573 (6.2)	3	567 (-4.1)	1
ECL3	651-654	651 (3.8)	1	654 (-4.2)	1
H8	682-693	687 (5.8), 683 (4.9), 686 (3.2)	3	693 (-3.7), 682 (-3.5), 685 (-3.3)	3

Interactive visualizations

ΔRRCS distribution

Active vs inactive comparison

Residue-wise changes

TM domain breakdown

Variants of interest

223 variants mapped to contact positions, sorted by |ΔRRCS| impact.

Position	Protein change	DNA change	Allele freq	Het / Hom	ΔRRCS	AM score	Pathogenicity	Conservation	dbSNP
657	p.Ser657Gly	c.1969A>G	6.84e-07	1 / 0	12.89	0.213	BENIGN	0.62	rs750049492
657	p.Ser657Thr	c.1970G>C	6.84e-07	1 / 0	12.89	-	nan	-
626	p.Met626Val	c.1876A>G	1.30e-05	19 / 0	12.10	0.805	PATHOGENIC	0.99	rs1252651159
626	p.Met626Leu	c.1876A>T	6.84e-07	1 / 0	12.10	-	nan	-
626	p.Met626Thr	c.1877T>C	6.84e-07	1 / 0	12.10	-	nan	-	rs778963363
38	p.Arg38Gly	c.112A>G	7.52e-06	11 / 0	11.80	0.309	BENIGN	0.49
34	p.Glu34Lys	c.100G>A	2.79e-04	406 / 1	11.80	0.088	BENIGN	0.43	rs45499704
34	p.Glu34Val	c.101A>T	6.84e-07	1 / 0	11.80	-	nan	-	rs1418754419
605	p.Tyr605His	c.1813T>C	6.57e-06	1 / 0	10.94	0.846	PATHOGENIC	0.97	rs1891817323
609	p.Arg609Gln	c.1826G>A	4.24e-05	62 / 0	10.94	0.095	BENIGN	0.61	rs139352934
676	p.Phe676Leu	c.2026T>C	1.37e-06	2 / 0	8.33	0.971	PATHOGENIC	0.86	rs779447985
676	p.Phe676Leu	c.2028C>A	2.05e-06	3 / 0	8.33	-	nan	-	rs141222082
405	p.Phe405Leu	c.1213T>C	2.05e-06	3 / 0	7.86	0.964	PATHOGENIC	0.85
405	p.Phe405Ser	c.1214T>C	6.84e-07	1 / 0	7.86	-	nan	-
621	p.Lys621Glu	c.1861A>G	6.84e-07	1 / 0	7.56	0.247	BENIGN	0.53
617	p.Asp617Glu	c.1851C>A	4.10e-06	6 / 0	7.56	0.135	BENIGN	0.42	rs367866466
621	p.Lys621Arg	c.1862A>G	6.57e-06	1 / 0	7.56	-	nan	-	rs1462191458
483	p.Asn483Ser	c.1448A>G	2.74e-06	4 / 0	7.53	0.168	BENIGN	0.77
483	p.Asn483Lys	c.1449C>A	6.84e-07	1 / 0	7.53	-	nan	-	rs2140111192
732	p.Leu732Ile	c.2194C>A	6.84e-07	1 / 0	7.50	0.070	BENIGN	0.67
732	p.Leu732Phe	c.2194C>T	6.84e-07	1 / 0	7.50	-	nan	-
135	p.Asn135Tyr	c.403A>T	2.06e-06	3 / 0	7.43	0.796	PATHOGENIC	0.63	rs1191546844
135	p.Asn135Thr	c.404A>C	2.74e-06	4 / 0	7.43	-	nan	-	rs1888696892
135	p.Asn135Ser	c.404A>G	6.85e-07	1 / 0	7.43	-	nan	-
135	p.Asn135Lys	c.405C>A	4.11e-06	6 / 0	7.43	-	nan	-	rs752457613
107	p.Glu107Asp	c.321A>C	1.37e-06	2 / 0	7.27	0.890	PATHOGENIC	0.43	rs771512572
109	p.Arg109Trp	c.325C>T	5.48e-06	8 / 0	7.27	0.558	AMBIGUOUS	0.48	rs777252114
109	p.Arg109Gln	c.326G>A	6.16e-06	9 / 0	7.27	-	nan	-	rs121908865
290	p.Lys290Asn	c.870G>T	2.94e-05	43 / 0	6.84	0.439	AMBIGUOUS	0.45	rs757787392
394	p.Glu394Lys	c.1180G>A	6.57e-06	1 / 0	6.84	0.081	BENIGN	0.40	rs2140110153
736	p.Glu736Asp	c.2208A>C	6.84e-07	1 / 0	6.59	0.089	BENIGN	0.54
735	p.Met735Val	c.2203A>G	3.42e-06	5 / 0	6.59	0.068	BENIGN	0.45	rs747999990
735	p.Met735Thr	c.2204T>C	2.05e-06	3 / 0	6.59	-	nan	-	rs1000765116
347	p.His347Asp	c.1039C>G	2.74e-06	4 / 0	6.47	0.071	BENIGN	0.40	rs1374886905
348	p.Asn348Asp	c.1042A>G	6.84e-07	1 / 0	6.47	0.065	BENIGN	0.41	rs2140109254
347	p.His347Tyr	c.1039C>T	2.05e-06	3 / 0	6.47	-	nan	-	rs1374886905
347	p.His347Arg	c.1040A>G	2.05e-06	3 / 0	6.47	-	nan	-	rs748660834
347	p.His347Gln	c.1041T>A	6.57e-06	1 / 0	6.47	-	nan	-	rs987999049
348	p.Asn348Ser	c.1043A>G	6.84e-07	1 / 0	6.47	-	nan	-	rs2140109271
339	p.Glu339Lys	c.1015G>A	1.31e-05	2 / 0	6.22	0.076	BENIGN	0.41	rs150821978
340	p.Lys340Glu	c.1018A>G	6.84e-07	1 / 0	6.22	0.070	BENIGN	0.34
339	p.Glu339Asp	c.1017A>T	6.84e-07	1 / 0	6.22	-	nan	-
340	p.Lys340Met	c.1019A>T	6.84e-07	1 / 0	6.22	-	nan	-	rs2140109093
340	p.Lys340Asn	c.1020G>C	7.52e-06	11 / 0	6.22	-	nan	-	rs767151716
739	p.Tyr739Cys	c.2216A>G	1.37e-06	2 / 0	6.18	0.066	BENIGN	0.53	rs1456730983
561	p.Ser561Ile	c.1682G>T	6.84e-07	1 / 0	6.17	0.944	PATHOGENIC	0.95
561	p.Ser561Thr	c.1682G>C	2.05e-06	3 / 0	6.17	-	nan	-	rs774590337
561	p.Ser561Asn	c.1682G>A	2.63e-05	4 / 0	6.17	-	nan	-	rs774590337
42	p.Lys42Glu	c.124A>G	2.05e-06	3 / 0	5.87	0.210	BENIGN	0.49	rs766387143
30	p.Glu30Lys	c.88G>A	2.74e-06	2 / 1	5.87	0.105	BENIGN	0.64
30	p.Glu30Gly	c.89A>G	6.84e-07	1 / 0	5.87	-	nan	-
30	p.Glu30Asp	c.90G>C	2.05e-06	3 / 0	5.87	-	nan	-
42	p.Lys42Arg	c.125A>G	6.84e-07	1 / 0	5.87	-	nan	-
680	p.Ile680Val	c.2038A>G	2.74e-06	4 / 0	5.87	0.123	BENIGN	0.76	rs771247662
444	p.Tyr444His	c.1330T>C	2.67e-05	37 / 1	5.76	0.157	BENIGN	0.47	rs754118014
687	p.Arg687Lys	c.2060G>A	2.05e-06	3 / 0	5.76	0.110	BENIGN	0.71
372	p.Asn372Thr	c.1115A>C	3.76e-05	55 / 0	5.71	0.402	AMBIGUOUS	0.50	rs1204330294
372	p.Asn372Lys	c.1116C>A	1.37e-06	2 / 0	5.71	-	nan	-	rs767633836
329	p.Asn329Ser	c.986A>G	6.84e-07	1 / 0	5.61	0.081	BENIGN	0.39
328	p.Glu328Asp	c.984G>C	2.05e-06	3 / 0	5.61	0.065	BENIGN	0.48	rs1231925448
328	p.Glu328Asp	c.984G>T	4.79e-06	7 / 0	5.61	-	nan	-	rs1231925448
263	p.Leu263Phe	c.787C>T	6.57e-06	1 / 0	5.57	0.215	BENIGN	0.63	rs1268072796
519	p.Arg519Ser	c.1555C>A	6.84e-07	1 / 0	5.53	0.986	PATHOGENIC	0.96	rs756016910
678	p.Tyr678Cys	c.2033A>G	6.84e-07	1 / 0	5.53	0.940	PATHOGENIC	0.99	rs758475693
519	p.Arg519Cys	c.1555C>T	1.23e-05	18 / 0	5.53	-	nan	-	rs756016910
519	p.Arg519His	c.1556G>A	2.53e-05	37 / 0	5.53	-	nan	-	rs780018604
130	p.Phe130Ser	c.389T>C	2.74e-06	4 / 0	5.51	0.914	PATHOGENIC	0.43
105	p.His105Asp	c.313C>G	2.05e-06	3 / 0	5.51	0.796	PATHOGENIC	0.45	rs765913152
390	p.Cys390Phe	c.1169G>T	3.22e-05	47 / 0	5.46	0.889	PATHOGENIC	0.80	rs371139156
293	p.Arg293Ser	c.879A>C	1.37e-06	2 / 0	5.46	0.234	BENIGN	0.31
390	p.Cys390Trp	c.1170T>G	7.66e-05	112 / 0	5.46	-	nan	-	rs121908871
484	p.His484Arg	c.1451A>G	1.37e-06	2 / 0	5.24	0.197	BENIGN	0.71	rs1376761472
484	p.His484Gln	c.1452T>G	6.84e-07	1 / 0	5.24	-	nan	-
450	p.Arg450Cys	c.1348C>T	6.16e-06	9 / 0	5.11	0.535	AMBIGUOUS	0.84	rs1357358128
450	p.Arg450His	c.1349G>A	1.11e-04	161 / 1	5.11	-	nan	-	rs189261858
450	p.Arg450Leu	c.1349G>T	6.57e-06	1 / 0	5.11	-	nan	-	rs189261858
585	p.Phe585Leu	c.1755T>G	1.37e-06	2 / 0	5.09	0.141	BENIGN	0.40
589	p.Leu589Val	c.1765C>G	1.37e-06	2 / 0	5.09	0.088	BENIGN	0.55
670	p.Asn670Lys	c.2010C>G	2.05e-06	3 / 0	5.06	0.997	PATHOGENIC	0.99	rs2140113552
636	p.Cys636Gly	c.1906T>G	2.05e-06	1 / 1	5.06	0.784	PATHOGENIC	0.98	rs1891823429
729	p.Arg729Trp	c.2185A>T	2.05e-06	3 / 0	4.99	0.102	BENIGN	0.47	rs750689462
730	p.Gln730Lys	c.2188C>A	6.84e-07	1 / 0	4.99	0.065	BENIGN	0.57	rs2140114384
729	p.Arg729Lys	c.2186G>A	3.42e-06	5 / 0	4.99	-	nan	-	rs903686564
762	p.Thr762Met	c.2285C>T	1.09e-05	16 / 0	4.87	0.084	BENIGN	0.93	rs767693393
762	p.Thr762Lys	c.2285C>A	6.84e-07	1 / 0	4.87	-	nan	-
410	p.Asp410Asn	c.1228G>A	8.21e-06	12 / 0	4.85	0.728	PATHOGENIC	0.98	rs121908868
408	p.Cys408Arg	c.1222T>C	6.16e-06	9 / 0	4.82	0.995	PATHOGENIC	1.00	rs199702292
408	p.Cys408Tyr	c.1223G>A	1.37e-06	2 / 0	4.82	-	nan	-	rs1213004397
704	p.Gln704Leu	c.2111A>T	6.84e-07	1 / 0	4.79	0.103	BENIGN	0.48	rs1238269994
709	p.Gln709Lys	c.2125C>A	6.84e-07	1 / 0	4.79	0.093	BENIGN	0.57	rs1387820706
660	p.Lys660Glu	c.1978A>G	4.79e-06	7 / 0	4.64	0.982	PATHOGENIC	1.00	rs1891828795
411	p.Ile411Val	c.1231A>G	6.57e-06	1 / 0	4.64	0.122	BENIGN	0.71	rs1397760048
411	p.Ile411Thr	c.1232T>C	6.84e-07	1 / 0	4.64	-	nan	-
660	p.Lys660Thr	c.1979A>C	2.74e-06	4 / 0	4.64	-	nan	-
291	p.Lys291Ile	c.872A>T	6.84e-07	1 / 0	4.59	0.342	AMBIGUOUS	0.48	rs777323212
251	p.Tyr251Phe	c.752A>T	5.47e-06	8 / 0	4.55	0.829	PATHOGENIC	0.38	rs192880617
274	p.Arg274Gly	c.820C>G	2.05e-06	3 / 0	4.55	0.228	BENIGN	0.44	rs373712078
251	p.Met251Lys	c.752T>A	6.84e-07	1 / 0	4.55	-	nan	-
251	p.Met251Ile	c.753G>A	6.84e-07	1 / 0	4.55	-	nan	-
251	p.Glu251Gln	c.751G>C	6.84e-07	1 / 0	4.55	-	nan	-	rs746685925
274	p.Arg274Trp	c.820C>T	8.89e-06	13 / 0	4.55	-	nan	-	rs373712078
274	p.Arg274Gln	c.821G>A	2.33e-05	34 / 0	4.55	-	nan	-	rs367893332
274	p.Arg274Leu	c.821G>T	6.84e-07	1 / 0	4.55	-	nan	-	rs367893332
634	p.Phe634Cys	c.1901T>G	6.84e-06	10 / 0	4.41	0.855	PATHOGENIC	0.96	rs1891822824
634	p.Phe634Leu	c.1902C>A	1.37e-06	2 / 0	4.41	-	nan	-	rs1891822984
301	p.Cys301Arg	c.901T>C	1.37e-06	2 / 0	4.36	0.881	PATHOGENIC	0.48	rs763974794
301	p.Cys301Tyr	c.902G>A	6.84e-06	10 / 0	4.36	-	nan	-	rs751054919
33	p.Gln33Arg	c.98A>G	6.57e-06	1 / 0	4.34	0.070	BENIGN	0.48	rs1886635842
273	p.Thr273Lys	c.818C>A	6.57e-06	1 / 0	4.32	0.117	BENIGN	0.37	rs1389373815
219	p.Asp219Tyr	c.655G>T	6.84e-07	1 / 0	4.19	0.095	BENIGN	0.57	rs371658911
244	p.Lys244Arg	c.731A>G	1.23e-05	18 / 0	4.19	0.066	BENIGN	0.40	rs2140032222
219	p.Asp219Glu	c.657T>G	6.84e-07	1 / 0	4.19	-	nan	-
244	p.Lys244Asn	c.732G>T	6.84e-07	1 / 0	4.19	-	nan	-	rs1890070461
244	p.Lys244Asn	c.732G>C	6.84e-07	1 / 0	4.19	-	nan	-
244	p.Gln244Arg	c.731A>G	6.84e-07	1 / 0	4.19	-	nan	-	rs2140032486
244	p.Gln244His	c.732G>C	2.74e-06	4 / 0	4.19	-	nan	-	rs374565206
243	p.Cys243Arg	c.727T>C	1.37e-06	2 / 0	4.17	0.271	BENIGN	0.44	rs1477626389
268	p.Ser268Gly	c.802A>G	6.57e-06	1 / 0	4.17	0.085	BENIGN	0.40	rs535187721
243	p.Cys243Tyr	c.728G>A	6.84e-07	1 / 0	4.17	-	nan	-	rs753939060
243	p.Cys243Phe	c.728G>T	6.84e-07	1 / 0	4.17	-	nan	-
243	p.Thr243Ile	c.728C>T	1.37e-06	2 / 0	4.17	-	nan	-
243	p.Ser243Tyr	c.728C>A	6.84e-07	1 / 0	4.17	-	nan	-
243	p.Ser243Cys	c.728C>G	6.84e-07	1 / 0	4.17	-	nan	-
268	p.Ser268Arg	c.804T>A	6.84e-07	1 / 0	4.17	-	nan	-
654	p.Ile654Thr	c.1961T>C	4.10e-06	6 / 0	4.17	0.974	PATHOGENIC	0.94	rs1566836131
486	p.Ile486Thr	c.1457T>C	6.84e-07	1 / 0	4.08	0.558	AMBIGUOUS	0.66
261	p.Lys261Gln	c.781A>C	6.57e-06	1 / 0	4.08	0.383	AMBIGUOUS	0.78	rs1891605681
261	p.Lys261Arg	c.782A>G	6.84e-07	1 / 0	4.08	-	nan	-
464	p.Gly464Arg	c.1390G>A	6.84e-07	1 / 0	4.06	0.996	PATHOGENIC	0.97	rs2140110967
429	p.Leu429Phe	c.1285C>T	6.84e-07	1 / 0	4.06	0.193	BENIGN	0.64	rs1271370001
429	p.Leu429Ile	c.1285C>A	6.84e-07	1 / 0	4.06	-	nan	-
429	p.Leu429Val	c.1285C>G	6.84e-07	1 / 0	4.06	-	nan	-	rs1271370001
403	p.Asp403Asn	c.1207G>A	1.71e-05	25 / 0	4.06	0.284	BENIGN	0.86	rs565082329
403	p.Asp403His	c.1207G>C	6.57e-06	1 / 0	4.06	-	nan	-	rs565082329
403	p.Asp403Tyr	c.1207G>T	6.57e-06	1 / 0	4.06	-	nan	-
734	p.Asn734Thr	c.2201A>C	6.57e-06	1 / 0	4.04	0.074	BENIGN	0.55	rs1891843907
734	p.Asn734Ser	c.2201A>G	6.84e-07	1 / 0	4.04	-	nan	-
723	p.Lys723Asn	c.2169G>T	2.74e-06	4 / 0	4.01	0.119	BENIGN	0.55	rs1433610933
722	p.Gln722Glu	c.2164C>G	6.57e-06	1 / 0	4.01	0.075	BENIGN	0.57	rs1406691651
386	p.Asp386Gly	c.1157A>G	6.84e-07	1 / 0	3.98	0.214	BENIGN	0.58
304	p.Ser304Asn	c.911G>A	2.74e-06	4 / 0	3.98	0.138	BENIGN	0.43	rs745523456
386	p.Asp386Glu	c.1158C>G	4.10e-06	6 / 0	3.98	-	nan	-	rs1302205384
82	p.Pro82Thr	c.244C>A	4.06e-06	1 / 0	3.95	0.645	PATHOGENIC	0.41
490	p.Thr490Ala	c.1468A>G	4.11e-06	6 / 0	3.93	0.361	AMBIGUOUS	0.55
490	p.Thr490Ile	c.1469C>T	6.84e-07	1 / 0	3.93	-	nan	-
63	p.His63Tyr	c.187C>T	6.85e-07	1 / 0	3.92	0.101	BENIGN	0.50	rs1347835071
619	p.Asp619Asn	c.1855G>A	6.84e-07	1 / 0	3.89	0.717	PATHOGENIC	0.86
615	p.Pro615Ser	c.1843C>T	6.84e-07	1 / 0	3.89	0.124	BENIGN	0.46
615	p.Pro615Leu	c.1844C>T	6.84e-07	1 / 0	3.89	-	nan	-
635	p.Ile635Leu	c.1903A>C	6.84e-07	1 / 0	3.79	0.051	BENIGN	0.54	rs772262589
635	p.Ile635Thr	c.1904T>C	6.84e-07	1 / 0	3.79	-	nan	-
635	p.Ile635Met	c.1905A>G	1.09e-05	16 / 0	3.79	-	nan	-	rs773335213
129	p.Lys129Glu	c.385A>G	1.37e-06	2 / 0	3.77	0.701	PATHOGENIC	0.63
151	p.Asp151Glu	c.453T>A	6.85e-07	1 / 0	3.77	0.361	AMBIGUOUS	0.41	rs1439290597
287	p.Lys287Thr	c.860A>C	6.84e-07	1 / 0	3.76	0.246	BENIGN	0.53
287	p.Lys287Asn	c.861G>C	6.57e-06	1 / 0	3.76	-	nan	-	rs372126085
646	p.Ser646Pro	c.1936T>C	6.84e-07	1 / 0	3.76	0.912	PATHOGENIC	0.69	rs775408982
651	p.Lys651Gln	c.1951A>C	1.09e-05	16 / 0	3.76	0.098	BENIGN	0.36	rs1328134414
651	p.Lys651Asn	c.1953G>T	6.84e-07	1 / 0	3.76	-	nan	-	rs1891826850
51	p.Pro51Ala	c.151C>G	6.84e-07	1 / 0	3.70	0.210	BENIGN	0.62	rs752234113
25	p.Ser25Pro	c.73T>C	6.84e-07	1 / 0	3.70	0.047	BENIGN	0.47	rs1886633581
25	p.Ser25Trp	c.74C>G	2.74e-06	4 / 0	3.70	-	nan	-	rs1279964862
51	p.Pro51Ser	c.151C>T	6.84e-07	1 / 0	3.70	-	nan	-	rs752234113
51	p.Pro51Leu	c.152C>T	7.52e-06	11 / 0	3.70	-	nan	-	rs758059665
267	p.Leu267Ser	c.800T>C	3.42e-06	5 / 0	3.69	0.140	BENIGN	0.33	rs1278487169
530	p.Asp530His	c.1588G>C	6.84e-07	1 / 0	3.67	0.463	AMBIGUOUS	0.62
531	p.Arg531Gly	c.1591C>G	1.31e-05	2 / 0	3.67	0.281	BENIGN	0.71	rs139892516
531	p.Arg531Trp	c.1591C>T	6.57e-05	96 / 0	3.67	-	nan	-	rs139892516
531	p.Arg531Gln	c.1592G>A	5.13e-05	75 / 0	3.67	-	nan	-	rs750198847
693	p.Leu693Phe	c.2077C>T	2.05e-06	3 / 0	3.66	0.114	BENIGN	0.65
698	p.Ile698Phe	c.2092A>T	2.05e-06	3 / 0	3.66	0.062	BENIGN	0.46	rs755360771
698	p.Ile698Val	c.2092A>G	6.84e-07	1 / 0	3.66	-	nan	-	rs755360771
698	p.Ile698Thr	c.2093T>C	6.84e-07	1 / 0	3.66	-	nan	-	rs1414044226
406	p.Asn406Ile	c.1217A>T	6.57e-06	1 / 0	3.64	0.814	PATHOGENIC	0.88	rs1891778465
297	p.Glu297Gly	c.890A>G	6.84e-07	1 / 0	3.60	0.172	BENIGN	0.50
385	p.Tyr385Cys	c.1154A>G	2.74e-06	4 / 0	3.54	0.348	AMBIGUOUS	0.49	rs1224730822
383	p.Ser383Cys	c.1147A>T	3.42e-06	5 / 0	3.54	0.253	BENIGN	0.43	rs1891773859
383	p.Ser383Asn	c.1148G>A	1.37e-06	2 / 0	3.54	-	nan	-	rs1375916178
480	p.Glu480Ala	c.1439A>C	6.84e-07	1 / 0	3.54	0.239	BENIGN	0.55
480	p.Glu480Asp	c.1440G>C	6.84e-07	1 / 0	3.54	-	nan	-	rs1891791816
159	p.Thr159Ala	c.475A>G	6.84e-07	1 / 0	3.51	0.085	BENIGN	0.41	rs1334238684
488	p.Trp488Arg	c.1462T>C	6.84e-07	1 / 0	3.51	0.999	PATHOGENIC	0.97	rs2140111252
488	p.Trp488Gly	c.1462T>G	6.84e-07	1 / 0	3.51	-	nan	-
395	p.Asp395Ala	c.1184A>C	6.84e-07	1 / 0	3.49	0.107	BENIGN	0.42
393	p.Ser393Gly	c.1177A>G	6.84e-07	1 / 0	3.49	0.070	BENIGN	0.35	rs747039454
393	p.Ser393Asn	c.1178G>A	6.84e-07	1 / 0	3.49	-	nan	-	rs1452747853
518	p.Glu518Val	c.1553A>T	6.84e-07	1 / 0	3.49	0.960	PATHOGENIC	0.97	rs767327783
518	p.Glu518Asp	c.1554G>C	6.84e-07	1 / 0	3.49	-	nan	-	rs750282571
474	p.Asp474Glu	c.1422C>A	1.16e-05	17 / 0	3.48	0.988	PATHOGENIC	1.00
418	p.Arg418Thr	c.1253G>C	6.84e-07	1 / 0	3.48	0.901	PATHOGENIC	0.98
718	p.Asp718Ala	c.2153A>C	1.37e-06	2 / 0	3.46	0.073	BENIGN	0.42
718	p.Asp718Gly	c.2153A>G	6.84e-07	1 / 0	3.46	-	nan	-
682	p.Thr682Ile	c.2045C>T	2.05e-06	3 / 0	3.46	0.980	PATHOGENIC	0.98	rs1290916925
392	p.Asp392Asn	c.1174G>A	1.09e-05	16 / 0	3.42	0.082	BENIGN	0.46	rs201396672
711	p.Val711Ile	c.2131G>A	2.74e-06	2 / 1	3.39	0.074	BENIGN	0.51	rs2140114115
373	p.Pro373Arg	c.1118C>G	2.63e-05	4 / 0	3.36	0.242	BENIGN	0.60	rs749925257
666	p.Phe666Leu	c.1996T>C	2.05e-06	3 / 0	3.33	0.956	PATHOGENIC	0.84
738	p.Val738Phe	c.2212G>T	1.37e-06	2 / 0	3.32	0.077	BENIGN	0.32
738	p.Val738Ile	c.2212G>A	6.84e-07	1 / 0	3.32	-	nan	-	rs1891844286
508	p.Ser508Leu	c.1523C>T	5.48e-06	8 / 0	3.31	0.920	PATHOGENIC	0.98	rs772490052
98	p.Tyr98Cys	c.293A>G	6.84e-07	1 / 0	3.25	0.101	BENIGN	0.36	rs2139910203
124	p.Glu124Asp	c.372G>T	4.65e-05	68 / 0	3.25	0.095	BENIGN	0.45	rs146496347
343	p.Phe343Leu	c.1027T>C	6.84e-07	1 / 0	3.24	0.293	BENIGN	0.38
345	p.Asp345Asn	c.1033G>A	1.98e-05	29 / 0	3.24	0.073	BENIGN	0.53	rs376188008
345	p.Asp345Tyr	c.1033G>T	3.42e-06	5 / 0	3.24	-	nan	-
345	p.Asp345His	c.1033G>C	6.84e-07	1 / 0	3.24	-	nan	-	rs376188008
255	p.Arg255Gly	c.763A>G	1.37e-06	2 / 0	3.23	0.159	BENIGN	0.39	rs1356767261
276	p.Asp276Asn	c.826G>A	1.37e-06	2 / 0	3.23	0.097	BENIGN	0.35	rs1891608431
255	p.Arg255Ile	c.764G>T	6.84e-07	1 / 0	3.23	-	nan	-
276	p.Asp276Glu	c.828C>A	6.84e-07	1 / 0	3.23	-	nan	-
686	p.Gln686His	c.2058G>T	6.84e-07	1 / 0	3.18	0.339	BENIGN	0.76
270	p.Leu270Pro	c.809T>C	6.84e-07	1 / 0	3.14	0.110	BENIGN	0.34
453	p.Met453Leu	c.1357A>C	1.37e-06	2 / 0	3.12	0.321	BENIGN	0.87
453	p.Met453Ile	c.1359G>C	6.84e-07	1 / 0	3.12	-	nan	-
389	p.Ile389Val	c.1165A>G	3.94e-05	6 / 0	3.08	0.059	BENIGN	0.48	rs377424991
389	p.Ile389Thr	c.1166T>C	5.47e-06	8 / 0	3.08	-	nan	-	rs1202308497
258	p.Trp258Arg	c.772T>C	6.84e-07	1 / 0	3.07	0.581	PATHOGENIC	0.34	rs1287622394
449	p.Pro449Ser	c.1345C>T	6.84e-07	1 / 0	3.04	0.189	BENIGN	0.72	rs1216365087
449	p.Pro449Leu	c.1346C>T	6.84e-07	1 / 0	3.04	-	nan	-
699	p.Cys699Arg	c.2095T>C	6.84e-07	1 / 0	3.02	0.576	PATHOGENIC	0.85	rs920050118
699	p.Cys699Tyr	c.2096G>A	1.37e-06	2 / 0	3.02	-	nan	-	rs2140113889
246	p.Leu246Phe	c.736C>T	3.42e-06	5 / 0	3.01	0.364	AMBIGUOUS	0.81	rs779236713
246	p.Ala246Thr	c.736G>A	2.46e-05	36 / 0	3.01	-	nan	-	rs376113420

Complete RRCS results

Res1	AA1	Res2	AA2	Active RRCS	Inactive RRCS	ΔRRCS	\|ΔRRCS\|
404	GLU	657	SER	0.000	12.886	-12.886	12.886
601	TYR	626	MET	1.202	13.307	-12.105	12.105
34	GLU	38	ARG	11.795	0.000	11.795	11.795
605	TYR	609	ARG	0.965	11.908	-10.943	10.943
601	TYR	630	ILE	2.110	11.552	-9.442	9.442
676	PHE	681	PHE	8.328	0.000	8.328	8.328
409	GLU	657	SER	8.299	0.000	8.299	8.299
404	GLU	656	VAL	1.595	9.760	-8.165	8.165
405	PHE	656	VAL	1.418	9.274	-7.856	7.856
617	ASP	621	LYS	0.000	7.561	-7.561	7.561
262	LYS	483	ASN	7.530	0.000	7.530	7.530
732	LEU	733	HIS	7.495	0.000	7.495	7.495
625	ARG	681	PHE	0.000	7.466	-7.466	7.466
112	ARG	135	ASN	0.583	8.017	-7.435	7.435
107	GLU	109	ARG	4.744	12.018	-7.274	7.274
643	TYR	659	SER	0.000	7.146	-7.146	7.146
405	PHE	565	LYS	6.911	0.000	6.911	6.911
290	LYS	394	GLU	6.845	0.000	6.845	6.845
735	MET	736	GLU	6.987	0.394	6.593	6.593
347	HIS	348	ASN	6.467	0.000	6.467	6.467
266	SER	289	GLN	0.000	6.423	-6.423	6.423
339	GLU	340	LYS	0.000	6.220	-6.220	6.220
739	TYR	740	GLU	6.179	0.000	6.179	6.179
561	SER	573	ASP	7.404	1.234	6.169	6.169
30	GLU	42	LYS	5.867	0.000	5.867	5.867
675	PRO	680	ILE	5.867	0.000	5.867	5.867
444	TYR	687	ARG	16.999	11.242	5.757	5.757
372	ASN	374	GLN	5.708	0.000	5.708	5.708
328	GLU	329	ASN	5.719	0.110	5.608	5.608
263	LEU	483	ASN	5.567	0.000	5.567	5.567
519	ARG	678	TYR	5.527	0.000	5.527	5.527
105	HIS	130	PHE	10.471	4.958	5.513	5.513
293	ARG	390	CYS	5.461	0.000	5.461	5.461
740	GLU	741	LEU	5.288	0.000	5.288	5.288
262	LYS	484	HIS	5.242	0.000	5.242	5.242
439	LEU	450	ARG	5.967	0.853	5.114	5.114
153	PHE	179	THR	0.058	5.148	-5.090	5.090
585	PHE	589	LEU	0.255	5.342	-5.087	5.087
636	CYS	670	ASN	7.240	2.185	5.055	5.055
729	ARG	730	GLN	5.073	0.085	4.988	4.988
683	LYS	762	THR	4.871	0.000	4.871	4.871
410	ASP	478	HIS	1.248	6.102	-4.855	4.855
408	CYS	482	TYR	0.000	4.822	-4.822	4.822
704	GLN	709	GLN	0.000	4.785	-4.785	4.785
411	ILE	660	LYS	5.708	1.072	4.636	4.636
291	LYS	394	GLU	4.595	0.000	4.595	4.595
423	PHE	427	LEU	0.000	4.552	-4.552	4.552
251	GLU	274	ARG	5.602	10.150	-4.548	4.548
404	GLU	660	LYS	0.000	4.543	-4.543	4.543
405	PHE	660	LYS	0.000	4.522	-4.522	4.522
594	PHE	634	PHE	5.555	9.960	-4.406	4.406
301	CYS	390	CYS	4.356	0.000	4.356	4.356
33	GLN	37	PHE	0.823	5.161	-4.338	4.338
273	THR	291	LYS	4.322	0.000	4.322	4.322
288	ASN	482	TYR	0.000	4.258	-4.258	4.258
105	HIS	107	GLU	4.200	0.000	4.200	4.200
219	ASP	244	LYS	5.281	1.087	4.194	4.194
243	SER	268	SER	0.000	4.170	-4.170	4.170
643	TYR	654	ILE	3.023	7.191	-4.167	4.167
409	GLU	660	LYS	4.153	0.000	4.153	4.153
482	TYR	483	ASN	4.052	8.175	-4.123	4.123
405	PHE	567	SER	0.032	4.140	-4.108	4.108
261	LYS	486	ILE	0.139	4.219	-4.081	4.081
444	TYR	450	ARG	0.000	4.067	-4.067	4.067
429	LEU	464	GLY	4.064	0.000	4.064	4.064
403	ASP	405	PHE	4.063	0.000	4.063	4.063
734	ASN	735	MET	4.037	0.000	4.037	4.037
722	GLN	723	LYS	4.009	0.000	4.009	4.009
304	SER	386	ASP	3.980	0.000	3.980	3.980
82	TYR	105	HIS	3.976	0.024	3.952	3.952
261	LYS	490	THR	0.000	3.932	-3.932	3.932
45	GLN	63	HIS	7.128	3.212	3.916	3.916
615	PRO	619	ASP	4.041	0.154	3.887	3.887
654	ILE	659	SER	4.212	0.337	3.875	3.875
279	TYR	486	ILE	5.575	1.760	3.816	3.816
631	PHE	635	ILE	3.789	0.000	3.789	3.789
129	LYS	151	ASP	4.016	7.788	-3.772	3.772
287	LYS	408	CYS	3.760	0.000	3.760	3.760
646	SER	651	LYS	10.525	6.768	3.757	3.757
25	SER	51	PRO	3.704	0.000	3.704	3.704
267	LEU	269	PHE	3.687	0.000	3.687	3.687
530	ASP	531	ARG	3.666	0.000	3.666	3.666
693	LEU	698	ILE	3.254	6.918	-3.664	3.664
403	ASP	406	ASN	3.638	0.000	3.638	3.638
293	ARG	297	GLU	1.383	4.985	-3.603	3.603
643	TYR	662	LEU	2.174	5.756	-3.582	3.582
383	SER	385	TYR	0.000	3.544	-3.544	3.544
676	PHE	680	ILE	1.454	4.998	-3.544	3.544
480	GLU	484	HIS	3.843	0.302	3.542	3.542
159	THR	185	TYR	8.203	11.712	-3.509	3.509
488	TRP	494	CYS	5.773	9.280	-3.507	3.507
393	SER	395	ASP	3.495	0.000	3.495	3.495
518	GLU	519	ARG	0.141	3.632	-3.490	3.490
418	ARG	474	ASP	9.502	6.023	3.479	3.479
289	GLN	396	MET	3.465	0.000	3.465	3.465
717	THR	718	ASP	3.457	0.000	3.457	3.457
678	TYR	682	THR	0.000	3.457	-3.457	3.457
291	LYS	392	ASP	3.420	0.000	3.420	3.420
291	LYS	395	ASP	3.400	0.000	3.400	3.400
704	GLN	711	VAL	0.000	3.394	-3.394	3.394
372	ASN	373	PRO	2.188	5.546	-3.358	3.358
291	LYS	396	MET	3.340	0.000	3.340	3.340
662	LEU	666	PHE	0.818	4.144	-3.327	3.327
737	ASP	738	VAL	3.320	0.000	3.320	3.320
460	ASP	508	SER	3.339	0.027	3.312	3.312
629	LEU	678	TYR	3.285	0.000	3.285	3.285
98	TYR	124	GLU	3.894	0.641	3.252	3.252
678	TYR	685	PHE	0.000	3.252	-3.252	3.252
343	PHE	345	ASP	3.486	0.243	3.243	3.243
680	ILE	685	PHE	3.231	0.000	3.231	3.231
255	ARG	276	ASP	9.921	6.691	3.230	3.230
218	LYS	219	ASP	0.000	3.212	-3.212	3.212
281	SER	482	TYR	4.395	1.193	3.202	3.202
683	LYS	686	GLN	3.341	0.159	3.182	3.182
261	LYS	279	TYR	3.260	0.085	3.175	3.175
594	PHE	598	CYS	3.159	0.000	3.159	3.159
519	ARG	601	TYR	3.158	0.000	3.158	3.158
265	LEU	480	GLU	3.154	0.000	3.154	3.154
270	LEU	289	GLN	3.141	0.000	3.141	3.141
287	LYS	288	ASN	3.126	0.000	3.126	3.126
453	MET	678	TYR	3.054	6.175	-3.121	3.121
123	LYS	124	GLU	3.714	0.605	3.108	3.108
385	TYR	389	ILE	3.077	0.000	3.077	3.077
258	TRP	565	LYS	0.000	3.066	-3.066	3.066
418	ARG	478	HIS	9.694	6.633	3.061	3.061
449	PRO	518	GLU	3.296	0.257	3.039	3.039
693	LEU	699	CYS	3.024	0.000	3.024	3.024
246	LEU	268	SER	0.000	3.011	-3.011	3.011
406	ASN	567	SER	0.011	3.001	-2.990	2.990
512	LEU	678	TYR	2.976	0.000	2.976	2.976
443	HIS	695	LYS	4.990	2.014	2.976	2.976
670	ASN	674	ASN	0.852	3.828	-2.975	2.975
610	ASN	612	GLN	0.610	3.541	-2.932	2.932
458	PHE	546	TRP	4.428	7.352	-2.923	2.923
609	ARG	619	ASP	2.888	0.000	2.888	2.888
285	ALA	482	TYR	3.241	0.354	2.887	2.887
414	TYR	658	ASN	2.879	0.000	2.879	2.879
37	PHE	54	THR	3.290	6.168	-2.878	2.878
153	PHE	181	THR	0.000	2.877	-2.877	2.877
410	ASP	479	SER	5.207	2.333	2.874	2.874
562	SER	573	ASP	4.276	1.403	2.873	2.873
410	ASP	481	TYR	3.454	0.592	2.862	2.862
573	ASP	579	ALA	2.632	5.491	-2.859	2.859
104	THR	105	HIS	0.481	3.339	-2.858	2.858
247	GLU	268	SER	0.000	2.849	-2.849	2.849
519	ARG	622	ILE	0.000	2.833	-2.833	2.833
738	VAL	739	TYR	2.832	0.000	2.832	2.832
243	SER	267	LEU	2.826	0.000	2.826	2.826
293	ARG	393	SER	2.822	0.000	2.822	2.822
153	PHE	155	ILE	0.000	2.818	-2.818	2.818
542	MET	546	TRP	0.585	3.376	-2.792	2.792
270	LEU	292	ILE	2.763	0.000	2.763	2.763
594	PHE	633	ASP	2.759	0.000	2.759	2.759
166	SER	191	SER	6.646	3.892	2.754	2.754
416	PHE	417	LEU	3.618	0.880	2.738	2.738
429	LEU	465	MET	2.725	0.000	2.725	2.725
290	LYS	395	ASP	0.002	2.707	-2.705	2.705
280	PRO	402	SER	2.814	0.114	2.699	2.699
607	THR	613	TYR	0.000	2.668	-2.668	2.668
276	ASP	399	THR	6.244	8.907	-2.663	2.663
642	PHE	643	TYR	2.622	0.000	2.622	2.622
473	VAL	488	TRP	3.079	5.660	-2.581	2.581
166	SER	193	GLN	2.817	0.240	2.578	2.578
409	GLU	479	SER	0.000	2.513	-2.513	2.513
594	PHE	637	MET	2.507	0.000	2.507	2.507
565	LYS	575	GLU	2.847	5.333	-2.486	2.486
157	GLU	185	TYR	1.859	4.344	-2.485	2.485
269	PHE	289	GLN	2.478	0.000	2.478	2.478
733	HIS	734	ASN	6.762	4.286	2.476	2.476
157	GLU	183	LYS	10.541	8.065	2.476	2.476
37	PHE	51	PRO	0.000	2.466	-2.466	2.466
442	SER	688	ASP	5.081	2.629	2.451	2.451
263	LEU	282	HIS	3.052	0.601	2.451	2.451
456	LEU	674	ASN	3.658	1.230	2.428	2.428
573	ASP	576	THR	0.000	2.421	-2.421	2.421
32	HIS	34	GLU	2.397	0.000	2.397	2.397
69	SER	93	GLU	2.228	4.614	-2.386	2.386
721	VAL	722	GLN	2.954	0.569	2.384	2.384
72	PHE	97	PHE	2.703	5.086	-2.383	2.383
521	TYR	533	ILE	2.930	0.559	2.371	2.371
574	THR	580	LEU	2.519	0.150	2.369	2.369
521	TYR	531	ARG	3.346	5.696	-2.349	2.349
632	THR	669	LEU	2.347	0.000	2.347	2.347
506	GLU	590	ASN	6.253	3.908	2.345	2.345
406	ASN	566	VAL	0.000	2.337	-2.337	2.337
378	LEU	382	ASP	2.324	0.000	2.324	2.324
241	LEU	264	PRO	0.783	3.069	-2.286	2.286
119	PRO	146	LYS	5.244	2.961	2.283	2.283
293	ARG	392	ASP	2.281	0.000	2.281	2.281
725	THR	726	HIS	2.266	0.000	2.266	2.266
463	MET	667	TYR	4.231	6.495	-2.264	2.264
272	LEU	396	MET	4.306	2.051	2.255	2.255
246	LEU	267	LEU	2.254	0.000	2.254	2.254
692	LEU	696	PHE	2.794	0.540	2.254	2.254
407	PRO	660	LYS	0.000	2.251	-2.251	2.251
32	HIS	40	THR	2.249	0.000	2.249	2.249
282	HIS	486	ILE	0.000	2.239	-2.239	2.239
94	SER	120	ASP	1.800	4.038	-2.238	2.238
601	TYR	627	ALA	0.000	2.233	-2.233	2.233
274	ARG	276	ASP	8.736	6.508	2.228	2.228
66	THR	93	GLU	5.136	2.910	2.226	2.226
610	ASN	613	TYR	2.359	0.139	2.220	2.220
152	ILE	178	GLU	0.869	3.086	-2.217	2.217
286	PHE	396	MET	2.843	5.053	-2.210	2.210
407	PRO	409	GLU	2.208	0.000	2.208	2.208
679	ALA	685	PHE	5.151	2.948	2.203	2.203
68	PRO	93	GLU	1.624	3.811	-2.187	2.187
94	SER	95	HIS	0.923	3.107	-2.185	2.185
143	ASP	146	LYS	2.805	0.624	2.181	2.181
601	TYR	678	TYR	2.172	0.000	2.172	2.172
489	GLN	564	ALA	5.415	7.587	-2.171	2.171
635	ILE	666	PHE	2.807	0.645	2.162	2.162
149	SER	176	CYS	5.151	3.013	2.139	2.139
434	PHE	685	PHE	2.718	0.581	2.136	2.136
63	HIS	86	ASP	3.340	1.222	2.118	2.118
263	LEU	269	PHE	0.000	2.113	-2.113	2.113
67	ILE	96	SER	3.308	1.196	2.113	2.113
621	LYS	761	GLN	2.099	0.000	2.099	2.099
407	PRO	481	TYR	0.000	2.098	-2.098	2.098
511	THR	542	MET	2.098	0.000	2.098	2.098
694	SER	707	ARG	0.000	2.095	-2.095	2.095
637	MET	641	SER	2.094	0.000	2.094	2.094
65	ARG	90	GLN	6.338	4.249	2.090	2.090
515	ILE	626	MET	0.000	2.084	-2.084	2.084
258	TRP	279	TYR	8.971	6.893	2.079	2.079
92	LEU	117	ILE	3.535	1.457	2.078	2.078
636	CYS	669	LEU	2.074	0.000	2.074	2.074
327	GLU	328	GLU	0.000	2.065	-2.065	2.065
466	TYR	663	LEU	2.064	0.000	2.064	2.064
747	LEU	748	THR	2.058	0.000	2.058	2.058
573	ASP	583	ILE	0.272	2.323	-2.052	2.052
601	TYR	629	LEU	2.050	0.000	2.050	2.050
467	LEU	668	PRO	1.251	3.297	-2.046	2.046
476	TYR	484	HIS	2.043	0.000	2.043	2.043
745	SER	746	HIS	2.042	0.000	2.042	2.042
28	PRO	42	LYS	1.138	3.180	-2.042	2.042
412	MET	660	LYS	2.025	0.000	2.025	2.025
687	ARG	706	TYR	0.000	2.021	-2.021	2.021
203	ASP	229	SER	9.571	7.552	2.019	2.019
481	TYR	489	GLN	2.794	0.789	2.005	2.005
410	ASP	418	ARG	6.821	4.826	1.995	1.995
723	LYS	724	VAL	1.989	0.000	1.989	1.989
466	TYR	568	ILE	2.598	4.585	-1.987	1.987
566	VAL	572	MET	2.400	0.415	1.985	1.985
587	LEU	641	SER	0.843	2.822	-1.979	1.979
529	LEU	532	LYS	0.478	2.455	-1.977	1.977
392	ASP	397	VAL	1.963	0.000	1.963	1.963
120	ASP	123	LYS	3.231	1.269	1.961	1.961
15	LEU	17	ARG	0.000	1.953	-1.953	1.953
620	THR	624	LYS	1.943	0.000	1.943	1.943
582	TYR	583	ILE	2.170	0.235	1.934	1.934
556	PRO	561	SER	5.856	7.776	-1.921	1.921
480	GLU	483	ASN	2.952	1.035	1.918	1.918
662	LEU	667	TYR	2.358	0.451	1.906	1.906
410	ASP	660	LYS	1.902	0.000	1.902	1.902
384	HIS	388	THR	1.901	0.000	1.901	1.901
172	PHE	197	PHE	3.016	4.912	-1.896	1.896
337	TYR	338	LYS	0.514	2.404	-1.890	1.890
85	ILE	110	ASN	1.201	3.090	-1.889	1.889
283	CYS	399	THR	0.122	2.010	-1.888	1.888
408	CYS	481	TYR	0.000	1.869	-1.869	1.869
79	SER	104	THR	5.705	7.569	-1.864	1.864
534	ARG	537	HIS	7.248	9.109	-1.861	1.861
126	PRO	150	THR	5.363	7.221	-1.858	1.858
516	THR	601	TYR	6.469	4.612	1.857	1.857
404	GLU	655	THR	0.000	1.856	-1.856	1.856
679	ALA	686	GLN	0.000	1.844	-1.844	1.844
334	ILE	335	VAL	0.732	2.572	-1.840	1.840
556	PRO	582	TYR	4.460	2.627	1.832	1.832
613	TYR	615	PRO	0.000	1.830	-1.830	1.830
283	CYS	398	CYS	8.815	6.989	1.826	1.826
272	LEU	286	PHE	2.605	0.790	1.815	1.815
412	MET	474	ASP	7.996	6.191	1.805	1.805
268	SER	289	GLN	1.797	0.000	1.797	1.797
251	GLU	253	ILE	0.000	1.795	-1.795	1.795
74	ASN	75	LEU	0.397	2.188	-1.792	1.792
515	ILE	519	ARG	1.787	0.000	1.787	1.787
199	GLY	225	TYR	6.825	5.041	1.784	1.784
55	GLN	79	SER	6.197	4.415	1.783	1.783
273	THR	397	VAL	1.369	3.147	-1.779	1.779
456	LEU	508	SER	2.877	1.100	1.777	1.777
52	PRO	74	ASN	2.487	0.715	1.772	1.772
139	LYS	165	THR	0.563	2.329	-1.766	1.766
406	ASN	565	LYS	1.487	3.251	-1.765	1.765
96	SER	121	ALA	3.652	5.410	-1.758	1.758
434	PHE	438	ILE	2.862	4.604	-1.743	1.743
269	PHE	286	PHE	2.031	0.297	1.734	1.734
677	LEU	681	PHE	0.114	1.846	-1.732	1.732
499	PHE	553	ALA	6.596	8.317	-1.721	1.721
639	PRO	662	LEU	0.000	1.715	-1.715	1.715
417	LEU	660	LYS	1.697	0.000	1.697	1.697
727	ASP	728	MET	1.695	0.000	1.695	1.695
636	CYS	667	TYR	1.695	0.000	1.695	1.695
247	GLU	270	LEU	0.000	1.689	-1.689	1.689
629	LEU	633	ASP	0.000	1.684	-1.684	1.684
448	VAL	518	GLU	2.208	0.528	1.680	1.680
201	LYS	226	SER	4.010	2.332	1.678	1.678
333	SER	334	ILE	1.537	3.213	-1.676	1.676
230	LEU	251	GLU	4.579	6.242	-1.663	1.663
505	SER	667	TYR	1.806	3.452	-1.646	1.646
690	PHE	702	GLN	0.000	1.645	-1.645	1.645
542	MET	543	VAL	0.000	1.638	-1.638	1.638
453	MET	679	ALA	1.635	0.000	1.635	1.635
643	TYR	653	LEU	1.632	0.000	1.632	1.632
30	GLU	40	THR	8.482	6.851	1.630	1.630
95	HIS	98	TYR	6.007	4.382	1.625	1.625
519	ARG	523	ILE	0.000	1.617	-1.617	1.617
647	ALA	652	PRO	1.613	0.000	1.613	1.613
262	LYS	282	HIS	1.698	0.093	1.605	1.605
164	MET	187	ASN	1.599	0.000	1.599	1.599
37	PHE	39	VAL	0.000	1.597	-1.597	1.597
746	HIS	747	LEU	1.597	0.000	1.597	1.597
694	SER	703	ALA	0.000	1.596	-1.596	1.596
110	ASN	135	ASN	8.492	6.897	1.594	1.594
289	GLN	395	ASP	1.593	0.000	1.593	1.593
280	PRO	400	PRO	4.224	2.632	1.591	1.591
170	ASN	173	GLN	2.969	4.553	-1.583	1.583
24	CYS	31	CYS	5.400	3.830	1.570	1.570
286	PHE	289	GLN	1.782	0.213	1.568	1.568
570	LEU	663	LEU	0.629	2.196	-1.567	1.567
425	SER	467	LEU	2.487	0.922	1.565	1.565
435	VAL	679	ALA	1.562	0.000	1.562	1.562
37	PHE	56	THR	1.735	3.284	-1.549	1.549
674	ASN	678	TYR	1.546	0.000	1.546	1.546
605	TYR	623	ALA	1.545	0.000	1.545	1.545
372	ASN	375	GLU	1.533	0.000	1.533	1.533
690	PHE	706	TYR	0.000	1.522	-1.522	1.522
414	TYR	657	SER	1.508	0.000	1.508	1.508
409	GLU	481	TYR	2.017	3.525	-1.508	1.508
340	LYS	341	SER	0.000	1.497	-1.497	1.497
280	PRO	406	ASN	0.000	1.490	-1.490	1.490
250	LYS	274	ARG	2.045	3.531	-1.486	1.486
594	PHE	630	ILE	1.597	0.112	1.485	1.485
452	LEU	542	MET	1.485	0.000	1.485	1.485
563	TYR	570	LEU	1.387	2.866	-1.479	1.479
25	SER	49	SER	1.479	0.000	1.479	1.479
412	MET	421	VAL	2.113	0.635	1.478	1.478
555	LEU	560	ILE	7.012	5.536	1.476	1.476
622	ILE	626	MET	1.463	0.000	1.463	1.463
43	ASP	61	GLU	2.547	4.010	-1.463	1.463
54	THR	75	LEU	1.813	0.362	1.450	1.450
736	GLU	737	ASP	2.227	0.777	1.450	1.450
429	LEU	461	PHE	3.214	1.768	1.446	1.446
320	SER	321	PRO	0.571	2.012	-1.441	1.441
633	ASP	670	ASN	5.587	7.022	-1.435	1.435
24	CYS	33	GLN	1.429	0.000	1.429	1.429
169	VAL	170	ASN	0.345	1.771	-1.426	1.426
203	ASP	228	PRO	2.733	1.308	1.426	1.426
691	ILE	707	ARG	0.000	1.423	-1.423	1.423
189	PHE	210	ASN	3.073	1.654	1.418	1.418
97	PHE	106	ILE	2.892	1.475	1.416	1.416
159	THR	160	ASP	3.381	1.970	1.411	1.411
456	LEU	511	THR	3.201	1.799	1.402	1.402
563	TYR	569	CYS	4.524	5.923	-1.399	1.399
451	PHE	535	LEU	1.678	0.283	1.394	1.394
726	HIS	727	ASP	8.085	6.694	1.391	1.391
646	SER	653	LEU	3.064	1.676	1.388	1.388
478	HIS	479	SER	3.198	1.812	1.386	1.386
94	SER	118	ASP	5.625	7.010	-1.385	1.385
249	LEU	272	LEU	3.806	5.190	-1.385	1.385
629	LEU	677	LEU	1.384	0.000	1.384	1.384
417	LEU	665	LEU	0.000	1.377	-1.377	1.377
466	TYR	497	ALA	4.233	5.608	-1.375	1.375
323	HIS	324	GLN	0.000	1.374	-1.374	1.374
197	PHE	221	PHE	5.829	4.455	1.374	1.374
451	PHE	542	MET	1.748	3.119	-1.371	1.371
506	GLU	586	VAL	2.549	1.181	1.368	1.368
509	VAL	590	ASN	3.384	2.017	1.367	1.367
375	GLU	376	GLU	0.000	1.361	-1.361	1.361
412	MET	417	LEU	2.403	1.046	1.357	1.357
552	LEU	582	TYR	4.647	3.291	1.356	1.356
79	SER	103	VAL	2.784	4.130	-1.347	1.347
273	THR	292	ILE	1.345	0.000	1.345	1.345
447	ASN	450	ARG	3.718	5.059	-1.341	1.341
342	LYS	343	PHE	1.340	0.000	1.340	1.340
625	ARG	682	THR	0.000	1.338	-1.338	1.338
39	VAL	54	THR	1.384	2.717	-1.332	1.332
128	LEU	154	PHE	5.001	6.331	-1.331	1.331
424	VAL	664	VAL	1.329	0.000	1.329	1.329
144	LEU	175	LEU	3.612	2.291	1.321	1.321
178	GLU	201	LYS	9.357	8.041	1.316	1.316
720	GLN	721	VAL	1.316	0.000	1.316	1.316
26	SER	48	PRO	0.000	1.309	-1.309	1.309
510	TYR	545	GLY	5.658	6.967	-1.308	1.308
22	MET	52	PRO	1.298	0.000	1.298	1.298
489	GLN	567	SER	2.334	1.038	1.297	1.297
97	PHE	125	LEU	2.858	1.562	1.296	1.296
642	PHE	646	SER	0.000	1.291	-1.291	1.291
243	SER	266	SER	1.289	0.000	1.289	1.289
261	LYS	282	HIS	3.887	2.599	1.288	1.288
200	THR	225	TYR	6.179	4.892	1.288	1.288
158	ILE	187	ASN	4.298	5.579	-1.282	1.282
44	ILE	64	LEU	3.951	2.676	1.274	1.274
32	HIS	38	ARG	8.634	7.360	1.274	1.274
455	ASN	542	MET	3.185	1.913	1.272	1.272
250	LYS	297	GLU	1.268	0.000	1.268	1.268
403	ASP	565	LYS	1.268	0.000	1.268	1.268
116	TYR	118	ASP	5.017	3.754	1.263	1.263
459	ALA	504	ALA	3.321	2.062	1.258	1.258
386	ASP	391	GLY	1.257	0.000	1.257	1.257
274	ARG	397	VAL	2.164	3.420	-1.255	1.255
256	ASN	258	TRP	1.537	0.284	1.252	1.252
279	TYR	490	THR	0.000	1.251	-1.251	1.251
582	TYR	586	VAL	1.180	2.425	-1.245	1.245
141	PHE	164	MET	1.867	3.105	-1.238	1.238
568	ILE	663	LEU	1.650	0.420	1.230	1.230
86	ASP	110	ASN	0.348	1.578	-1.230	1.230
506	GLU	552	LEU	0.530	1.759	-1.229	1.229
495	ASN	563	TYR	3.993	2.766	1.227	1.227
420	VAL	664	VAL	1.227	0.000	1.227	1.227
285	ALA	286	PHE	1.222	0.000	1.222	1.222
640	ILE	667	TYR	3.698	2.476	1.222	1.222
528	ARG	530	ASP	1.217	0.000	1.217	1.217
61	GLU	85	ILE	4.256	5.461	-1.205	1.205
266	SER	267	LEU	1.195	0.000	1.195	1.195
591	ILE	641	SER	1.193	0.000	1.193	1.193
450	ARG	688	ASP	4.513	3.323	1.190	1.190
463	MET	671	SER	2.006	0.826	1.181	1.181
425	SER	464	GLY	2.444	1.267	1.178	1.178
341	SER	342	LYS	1.177	0.000	1.177	1.177
565	LYS	573	ASP	1.380	2.555	-1.176	1.176
632	THR	670	ASN	2.104	3.276	-1.172	1.172
419	ILE	423	PHE	1.168	0.000	1.168	1.168
407	PRO	567	SER	0.000	1.167	-1.167	1.167
411	ILE	567	SER	0.674	1.836	-1.162	1.162
716	SER	717	THR	1.635	0.475	1.159	1.159
69	SER	94	SER	5.455	4.296	1.158	1.158
453	MET	674	ASN	1.155	0.000	1.155	1.155
63	HIS	88	THR	4.708	5.859	-1.151	1.151
512	LEU	633	ASP	2.847	3.996	-1.149	1.149
149	SER	177	ASN	1.925	0.778	1.147	1.147
444	TYR	691	ILE	1.272	0.128	1.144	1.144
663	LEU	668	PRO	1.929	0.787	1.142	1.142
258	TRP	575	GLU	0.000	1.133	-1.133	1.133
185	TYR	210	ASN	1.826	0.694	1.132	1.132
161	ASN	187	ASN	9.921	8.790	1.130	1.130
264	PRO	267	LEU	1.130	0.000	1.130	1.130
250	LYS	272	LEU	5.553	4.423	1.130	1.130
411	ILE	474	ASP	4.980	6.108	-1.128	1.128
570	LEU	572	MET	1.318	0.196	1.123	1.123
270	LEU	271	HIS	0.090	1.212	-1.122	1.122
681	PHE	686	GLN	1.117	0.000	1.117	1.117
92	LEU	114	LEU	0.737	1.854	-1.117	1.117
574	THR	579	ALA	1.116	0.000	1.116	1.116
741	LEU	742	ILE	1.115	0.000	1.115	1.115
587	LEU	591	ILE	0.000	1.106	-1.106	1.106
608	VAL	615	PRO	0.000	1.105	-1.105	1.105
405	PHE	572	MET	1.104	0.000	1.104	1.104
154	PHE	180	LEU	3.350	4.454	-1.104	1.104
351	HIS	353	TYR	0.000	1.103	-1.103	1.103
461	PHE	465	MET	2.568	3.670	-1.102	1.102
145	THR	174	GLY	3.865	2.768	1.097	1.097
439	LEU	453	MET	1.642	0.546	1.097	1.097
184	LEU	206	TYR	4.815	5.910	-1.096	1.096
72	PHE	81	ILE	1.775	0.681	1.094	1.094
109	ARG	110	ASN	1.315	0.223	1.092	1.092
690	PHE	703	ALA	0.000	1.090	-1.090	1.090
439	LEU	685	PHE	1.252	0.163	1.089	1.089
31	CYS	39	VAL	0.645	1.733	-1.088	1.088
56	THR	78	ILE	1.087	0.000	1.087	1.087
724	VAL	725	THR	1.086	0.000	1.086	1.086
50	LEU	75	LEU	1.459	2.545	-1.086	1.086
247	GLU	267	LEU	1.085	0.000	1.085	1.085
568	ILE	570	LEU	1.116	0.034	1.082	1.082
466	TYR	501	THR	9.856	8.775	1.081	1.081
610	ASN	619	ASP	1.079	0.000	1.079	1.079
51	PRO	75	LEU	0.410	1.484	-1.074	1.074
217	ASP	218	LYS	0.353	1.426	-1.073	1.073
610	ASN	615	PRO	1.070	0.000	1.070	1.070
604	ILE	626	MET	1.070	0.000	1.070	1.070
280	PRO	401	LYS	1.358	0.288	1.070	1.070
618	LYS	622	ILE	0.000	1.066	-1.066	1.066
425	SER	429	LEU	1.060	0.000	1.060	1.060
417	LEU	661	ILE	1.362	0.304	1.059	1.059
326	TYR	327	GLU	0.373	1.430	-1.057	1.057
151	ASP	153	PHE	1.048	0.000	1.048	1.048
414	TYR	416	PHE	2.939	3.981	-1.042	1.042
411	ILE	473	VAL	1.798	0.760	1.038	1.038
151	ASP	178	GLU	0.452	1.490	-1.038	1.038
654	ILE	662	LEU	1.036	0.000	1.036	1.036
389	ILE	390	CYS	1.035	0.000	1.035	1.035
456	LEU	460	ASP	1.913	0.879	1.034	1.034
180	LEU	202	LEU	1.794	2.827	-1.033	1.033
512	LEU	629	LEU	0.000	1.032	-1.032	1.032
44	ILE	63	HIS	6.153	5.122	1.031	1.031
154	PHE	179	THR	2.264	3.292	-1.028	1.028
643	TYR	658	ASN	0.000	1.027	-1.027	1.027
406	ASN	489	GLN	3.791	2.766	1.025	1.025
111	THR	135	ASN	1.988	3.010	-1.022	1.022
613	TYR	619	ASP	0.000	1.022	-1.022	1.022
597	VAL	630	ILE	0.000	1.020	-1.020	1.020
591	ILE	637	MET	1.019	0.000	1.019	1.019
36	ASP	55	GLN	2.967	3.983	-1.016	1.016
499	PHE	500	PHE	0.000	1.009	-1.009	1.009
694	SER	699	CYS	1.007	0.000	1.007	1.007
414	TYR	417	LEU	1.684	0.685	0.999	0.999
558	VAL	560	ILE	0.000	0.997	-0.997	0.997
556	PRO	563	TYR	5.996	6.988	-0.991	0.991
72	PHE	100	LEU	2.944	1.954	0.991	0.991
187	ASN	210	ASN	6.756	7.745	-0.988	0.988
439	LEU	678	TYR	0.000	0.988	-0.988	0.988
186	ASN	210	ASN	3.048	2.061	0.986	0.986
325	GLU	326	TYR	2.455	1.476	0.979	0.979
184	LEU	189	PHE	2.001	1.021	0.979	0.979
513	THR	593	ALA	2.393	1.418	0.975	0.975
52	PRO	77	ASN	1.090	2.059	-0.969	0.969
338	LYS	339	GLU	0.000	0.965	-0.965	0.965
206	TYR	230	LEU	2.746	1.784	0.962	0.962
147	VAL	175	LEU	3.858	2.900	0.959	0.959
510	TYR	542	MET	1.050	0.094	0.957	0.957
272	LEU	289	GLN	0.954	0.000	0.954	0.954
360	GLU	361	ASP	0.954	0.000	0.954	0.954
37	PHE	55	GLN	6.726	5.772	0.954	0.954
202	LEU	226	SER	4.855	3.902	0.953	0.953
324	GLN	325	GLU	4.808	3.855	0.953	0.953
179	THR	201	LYS	2.629	1.677	0.952	0.952
4	ALA	5	ASP	0.952	0.000	0.952	0.952
169	VAL	195	TYR	1.102	2.050	-0.949	0.949
161	ASN	164	MET	1.716	0.770	0.946	0.946
435	VAL	685	PHE	3.700	2.755	0.946	0.946
135	ASN	160	ASP	4.618	5.564	-0.945	0.945
56	THR	80	ARG	6.268	5.325	0.943	0.943
432	ASN	675	PRO	4.485	3.544	0.941	0.941
36	ASP	80	ARG	2.849	1.912	0.937	0.937
39	VAL	56	THR	2.980	3.915	-0.935	0.935
610	ASN	611	PRO	1.491	2.425	-0.934	0.934
133	ILE	158	ILE	3.804	2.871	0.933	0.933
448	VAL	533	ILE	1.224	0.292	0.932	0.932
456	LEU	512	LEU	0.931	0.000	0.931	0.931
181	THR	204	ALA	2.237	3.166	-0.929	0.929
427	LEU	672	CYS	1.420	0.492	0.929	0.929
436	LEU	457	ALA	2.255	1.331	0.924	0.924
584	VAL	648	ILE	0.682	1.605	-0.923	0.923
55	GLN	78	ILE	3.443	2.521	0.923	0.923
131	LEU	154	PHE	0.264	1.185	-0.922	0.922
503	PHE	549	CYS	2.571	3.492	-0.921	0.921
411	ILE	477	THR	1.012	0.100	0.913	0.913
261	LYS	483	ASN	0.912	0.000	0.912	0.912
414	TYR	661	ILE	0.453	1.364	-0.911	0.911
152	ILE	153	PHE	0.910	0.000	0.910	0.910
431	GLY	675	PRO	2.279	1.373	0.907	0.907
73	SER	74	ASN	0.906	0.000	0.906	0.906
147	VAL	174	GLY	1.638	0.736	0.903	0.903
112	ARG	136	THR	0.171	1.069	-0.897	0.897
663	LEU	667	TYR	0.989	0.093	0.896	0.896
97	PHE	122	LEU	2.129	3.022	-0.893	0.893
651	LYS	653	LEU	1.058	0.166	0.892	0.892
376	GLU	377	THR	0.000	0.892	-0.892	0.892
573	ASP	580	LEU	0.000	0.891	-0.891	0.891
488	TRP	493	GLY	4.654	3.765	0.889	0.889
687	ARG	688	ASP	1.925	1.037	0.888	0.888
167	ILE	193	GLN	3.329	4.216	-0.887	0.887
169	VAL	194	GLY	1.949	2.834	-0.885	0.885
283	CYS	400	PRO	1.175	2.055	-0.880	0.880
239	THR	259	THR	2.280	3.156	-0.876	0.876
354	VAL	356	PHE	1.380	0.509	0.871	0.871
728	MET	729	ARG	0.864	0.000	0.864	0.864
263	LEU	277	LEU	0.863	0.000	0.863	0.863
190	THR	213	LEU	4.457	3.599	0.859	0.859
198	ASN	223	GLY	4.393	5.251	-0.858	0.858
252	LEU	274	ARG	2.471	3.328	-0.857	0.857
45	GLN	64	LEU	1.413	2.268	-0.854	0.854
561	SER	579	ALA	1.794	2.646	-0.851	0.851
17	ARG	18	ASP	0.000	0.851	-0.851	0.851
519	ARG	629	LEU	0.851	0.000	0.851	0.851
571	PRO	583	ILE	2.774	1.925	0.849	0.849
515	ILE	678	TYR	0.847	0.000	0.847	0.847
26	SER	49	SER	0.000	0.841	-0.841	0.841
655	THR	658	ASN	2.714	3.555	-0.841	0.841
571	PRO	582	TYR	0.114	0.954	-0.840	0.840
553	ALA	563	TYR	3.977	3.138	0.839	0.839
145	THR	173	GLN	5.678	4.841	0.837	0.837
432	ASN	457	ALA	6.699	5.863	0.836	0.836
67	ILE	92	LEU	2.234	1.400	0.835	0.835
470	ILE	488	TRP	0.000	0.833	-0.833	0.833
477	THR	484	HIS	2.662	1.842	0.820	0.820
247	GLU	271	HIS	2.925	3.745	-0.819	0.819
106	ILE	131	LEU	3.034	3.853	-0.819	0.819
57	LEU	78	ILE	1.382	0.565	0.818	0.818
183	LYS	206	TYR	1.958	2.775	-0.818	0.818
458	PHE	507	LEU	0.276	1.091	-0.815	0.815
197	PHE	220	ALA	4.408	3.598	0.809	0.809
632	THR	677	LEU	1.772	0.963	0.809	0.809
122	LEU	147	VAL	0.979	1.785	-0.806	0.806
489	GLN	494	CYS	3.398	2.595	0.803	0.803
75	LEU	78	ILE	0.949	1.752	-0.803	0.803
66	THR	91	GLN	5.851	5.050	0.801	0.801
561	SER	571	PRO	0.967	1.768	-0.800	0.800
451	PHE	538	ALA	1.591	0.790	0.800	0.800
405	PHE	566	VAL	3.240	2.442	0.798	0.798
248	HIS	271	HIS	1.592	0.794	0.797	0.797
583	ILE	587	LEU	0.796	0.000	0.796	0.796
275	ALA	397	VAL	2.881	3.673	-0.792	0.792
148	TYR	174	GLY	5.860	5.069	0.791	0.791
555	LEU	582	TYR	1.991	2.781	-0.790	0.790
632	THR	673	ALA	0.790	0.000	0.790	0.790
109	ARG	134	PHE	17.600	16.811	0.788	0.788
505	SER	506	GLU	1.963	1.175	0.788	0.788
402	SER	408	CYS	0.443	1.228	-0.785	0.785
458	PHE	462	CYS	1.408	2.190	-0.782	0.782
719	ILE	720	GLN	0.782	0.000	0.782	0.782
677	LEU	678	TYR	0.780	0.000	0.780	0.780
275	ALA	399	THR	3.906	4.686	-0.780	0.780
421	VAL	471	ALA	1.311	0.535	0.775	0.775
698	ILE	699	CYS	0.000	0.774	-0.774	0.774
255	ARG	277	LEU	1.002	0.227	0.774	0.774
122	LEU	131	LEU	1.286	0.513	0.773	0.773
665	LEU	666	PHE	1.012	0.240	0.772	0.772
44	ILE	62	THR	3.348	4.116	-0.767	0.767
258	TRP	278	SER	3.447	2.681	0.767	0.767
557	LEU	563	TYR	1.955	1.192	0.763	0.763
112	ARG	137	GLY	2.982	3.744	-0.762	0.762
456	LEU	515	ILE	0.865	0.104	0.762	0.762
422	TRP	471	ALA	5.636	6.398	-0.762	0.762
270	LEU	293	ARG	0.000	0.762	-0.762	0.762
605	TYR	619	ASP	0.760	0.000	0.760	0.760
228	PRO	249	LEU	3.361	4.121	-0.760	0.760
438	ILE	692	LEU	0.979	0.220	0.758	0.758
587	LEU	645	LEU	1.558	0.802	0.756	0.756
264	PRO	269	PHE	2.167	2.922	-0.755	0.755
242	PRO	246	LEU	1.162	0.408	0.754	0.754
446	LEU	535	LEU	0.481	1.233	-0.752	0.752
58	LYS	82	TYR	2.876	3.625	-0.749	0.749
428	ALA	672	CYS	1.215	1.960	-0.745	0.745
250	LYS	273	THR	5.435	6.179	-0.744	0.744
117	ILE	138	LEU	1.944	2.687	-0.743	0.743
239	THR	262	LYS	0.000	0.742	-0.742	0.742
281	SER	282	HIS	0.982	0.242	0.740	0.740
231	LEU	252	LEU	2.612	3.352	-0.740	0.740
92	LEU	97	PHE	0.249	0.988	-0.739	0.739
521	TYR	525	PHE	5.276	4.538	0.738	0.738
466	TYR	470	ILE	4.101	3.364	0.737	0.737
499	PHE	550	PHE	2.344	3.079	-0.736	0.736
26	SER	27	PRO	0.834	0.100	0.734	0.734
523	ILE	613	TYR	0.000	0.734	-0.734	0.734
119	PRO	143	ASP	5.228	4.494	0.734	0.734
645	LEU	649	LEU	0.000	0.734	-0.734	0.734
277	LEU	400	PRO	2.017	2.750	-0.732	0.732
291	LYS	393	SER	0.731	0.000	0.731	0.731
421	VAL	664	VAL	1.923	1.192	0.731	0.731
100	LEU	124	GLU	0.594	1.325	-0.731	0.731
298	SER	302	ASN	0.729	0.000	0.729	0.729
58	LYS	60	ILE	1.118	0.391	0.727	0.727
64	LEU	88	THR	6.015	6.737	-0.723	0.723
680	ILE	686	GLN	2.549	1.829	0.720	0.720
506	GLU	589	LEU	0.720	0.000	0.720	0.720
412	MET	418	ARG	1.062	0.343	0.719	0.719
221	PHE	246	LEU	1.112	1.829	-0.716	0.716
252	LEU	277	LEU	0.187	0.903	-0.716	0.716
435	VAL	680	ILE	0.715	0.000	0.715	0.715
164	MET	189	PHE	6.836	7.552	-0.715	0.715
427	LEU	676	PHE	0.714	0.000	0.714	0.714
481	TYR	488	TRP	1.437	2.148	-0.711	0.711
154	PHE	175	LEU	3.631	2.920	0.711	0.711
235	GLN	257	THR	1.911	1.200	0.710	0.710
748	THR	749	PRO	0.457	1.168	-0.710	0.710
156	LEU	158	ILE	2.388	1.679	0.709	0.709
42	LYS	43	ASP	0.999	0.291	0.708	0.708
251	GLU	273	THR	0.701	0.000	0.701	0.701
122	LEU	144	LEU	0.000	0.696	-0.696	0.696
31	CYS	38	ARG	1.169	1.864	-0.696	0.696
568	ILE	660	LYS	0.645	1.341	-0.696	0.696
554	LEU	558	VAL	0.909	0.216	0.693	0.693
445	LYS	450	ARG	0.000	0.691	-0.691	0.691
98	TYR	123	LYS	5.708	6.399	-0.691	0.691
282	HIS	482	TYR	0.690	0.000	0.690	0.690
587	LEU	644	ALA	1.464	0.774	0.690	0.690
591	ILE	645	LEU	0.689	0.000	0.689	0.689
273	THR	394	GLU	0.000	0.687	-0.687	0.687
524	THR	525	PHE	1.138	0.455	0.683	0.683
435	VAL	453	MET	0.681	0.000	0.681	0.681
409	GLU	480	GLU	0.000	0.680	-0.680	0.680
640	ILE	663	LEU	0.000	0.679	-0.679	0.679
494	CYS	563	TYR	2.147	1.469	0.678	0.678
523	ILE	608	VAL	0.704	0.026	0.678	0.678
240	ALA	262	LYS	0.000	0.676	-0.676	0.676
566	VAL	568	ILE	0.676	0.000	0.676	0.676
528	ARG	531	ARG	1.056	0.381	0.675	0.675
680	ILE	681	PHE	0.000	0.675	-0.675	0.675
158	ILE	184	LEU	3.021	3.695	-0.675	0.675
346	THR	347	HIS	0.672	0.000	0.672	0.672
460	ASP	670	ASN	0.671	0.000	0.671	0.671
115	THR	138	LEU	2.405	3.074	-0.669	0.669
489	GLN	565	LYS	0.000	0.668	-0.668	0.668
598	CYS	630	ILE	0.667	0.000	0.667	0.667
463	MET	467	LEU	0.000	0.664	-0.664	0.664
149	SER	175	LEU	3.314	3.974	-0.660	0.660
80	ARG	82	TYR	2.544	1.884	0.659	0.659
158	ILE	185	TYR	1.256	1.915	-0.659	0.659
441	THR	695	LYS	0.658	0.000	0.658	0.658
168	PRO	193	GLN	0.644	1.294	-0.651	0.651
429	LEU	468	LEU	1.180	0.531	0.649	0.649
463	MET	505	SER	0.834	0.190	0.645	0.645
15	LEU	16	PRO	0.645	0.000	0.645	0.645
699	CYS	702	GLN	0.000	0.640	-0.640	0.640
436	LEU	458	PHE	1.145	0.508	0.638	0.638
51	PRO	53	SER	0.634	0.000	0.634	0.634
103	VAL	127	LEU	3.494	4.127	-0.633	0.633
234	SER	257	THR	2.288	1.656	0.632	0.632
410	ASP	474	ASP	1.176	1.807	-0.632	0.632
207	LEU	213	LEU	1.055	0.424	0.631	0.631
608	VAL	622	ILE	0.630	0.000	0.630	0.630
253	ILE	276	ASP	2.676	3.306	-0.629	0.629
238	VAL	259	THR	7.249	7.872	-0.623	0.623
561	SER	582	TYR	0.444	1.064	-0.621	0.621
265	LEU	483	ASN	0.620	0.000	0.620	0.620
642	PHE	654	ILE	0.000	0.616	-0.616	0.616
229	SER	249	LEU	2.733	2.118	0.615	0.615
508	SER	674	ASN	0.612	0.000	0.612	0.612
617	ASP	620	THR	0.000	0.610	-0.610	0.610
696	PHE	698	ILE	0.607	0.000	0.607	0.607
140	MET	164	MET	2.089	1.483	0.606	0.606
286	PHE	398	CYS	0.000	0.605	-0.605	0.605
448	VAL	535	LEU	1.574	2.179	-0.605	0.605
499	PHE	554	LEU	1.290	1.894	-0.604	0.604
131	LEU	155	ILE	2.230	2.834	-0.604	0.604
574	THR	583	ILE	0.846	0.244	0.602	0.602
496	THR	500	PHE	1.265	1.866	-0.601	0.601
190	THR	191	SER	1.433	0.833	0.600	0.600
572	MET	583	ILE	0.000	0.595	-0.595	0.595
177	ASN	201	LYS	3.685	3.093	0.593	0.593
572	MET	644	ALA	0.000	0.592	-0.592	0.592
554	LEU	557	LEU	0.000	0.592	-0.592	0.592
64	LEU	67	ILE	0.591	0.000	0.591	0.591
656	VAL	659	SER	0.000	0.589	-0.589	0.589
184	LEU	207	LEU	0.734	1.323	-0.589	0.589
562	SER	571	PRO	1.467	0.881	0.586	0.586
160	ASP	187	ASN	1.867	1.282	0.585	0.585
463	MET	504	ALA	2.136	2.719	-0.583	0.583
654	ILE	658	ASN	1.161	0.578	0.583	0.583
639	PRO	666	PHE	0.582	0.000	0.582	0.582
264	PRO	268	SER	0.000	0.582	-0.582	0.582
198	ASN	222	GLY	3.240	2.659	0.582	0.582
43	ASP	62	THR	0.880	0.299	0.581	0.581
130	PHE	157	GLU	2.393	1.813	0.580	0.580
509	VAL	637	MET	0.000	0.579	-0.579	0.579
207	LEU	232	ASP	4.122	4.701	-0.579	0.579
120	ASP	146	LYS	6.501	5.923	0.579	0.579
255	ARG	256	ASN	0.000	0.578	-0.578	0.578
66	THR	89	LEU	1.881	1.304	0.578	0.578
78	ILE	81	ILE	1.649	2.223	-0.574	0.574
208	ASN	236	THR	0.710	0.137	0.574	0.574
424	VAL	672	CYS	1.193	0.619	0.573	0.573
55	GLN	77	ASN	3.748	3.176	0.572	0.572
125	LEU	148	TYR	0.105	0.675	-0.570	0.570
233	VAL	257	THR	2.975	2.405	0.570	0.570
41	CYS	62	THR	1.227	0.657	0.570	0.570
131	LEU	156	LEU	3.972	4.539	-0.566	0.566
447	ASN	449	PRO	2.427	2.993	-0.566	0.566
634	PHE	635	ILE	0.566	0.000	0.566	0.566
127	LEU	151	ASP	0.148	0.710	-0.562	0.562
141	PHE	189	PHE	1.429	0.866	0.562	0.562
133	ILE	136	THR	1.027	1.587	-0.560	0.560
453	MET	675	PRO	0.558	0.000	0.558	0.558
185	TYR	206	TYR	1.716	1.160	0.556	0.556
32	HIS	37	PHE	0.739	1.294	-0.555	0.555
182	LEU	206	TYR	4.424	4.979	-0.555	0.555
86	ASP	89	LEU	1.979	2.533	-0.555	0.555
277	LEU	283	CYS	0.554	0.000	0.554	0.554
124	GLU	147	VAL	2.631	2.077	0.553	0.553
133	ILE	161	ASN	2.920	2.367	0.553	0.553
234	SER	254	ALA	3.010	2.459	0.551	0.551
62	THR	88	THR	0.465	1.013	-0.548	0.548
92	LEU	108	ILE	0.956	0.412	0.544	0.544
424	VAL	467	LEU	0.543	0.000	0.543	0.543
441	THR	692	LEU	0.239	0.781	-0.543	0.543
72	PHE	96	SER	1.735	2.276	-0.541	0.541
236	THR	257	THR	4.371	3.830	0.541	0.541
16	PRO	18	ASP	0.539	0.000	0.539	0.539
36	ASP	38	ARG	1.067	0.528	0.539	0.539
627	ALA	631	PHE	0.000	0.537	-0.537	0.537
634	PHE	638	ALA	2.497	1.960	0.537	0.537
115	THR	137	GLY	2.520	3.055	-0.536	0.536
146	LYS	174	GLY	0.535	0.000	0.535	0.535
167	ILE	189	PHE	3.364	2.831	0.533	0.533
425	SER	468	LEU	4.068	3.536	0.532	0.532
29	CYS	40	THR	2.382	2.913	-0.531	0.531
273	THR	395	ASP	0.000	0.531	-0.531	0.531
263	LEU	286	PHE	1.338	1.869	-0.531	0.531
274	ARG	396	MET	0.560	0.031	0.529	0.529
567	SER	660	LYS	1.185	1.712	-0.528	0.528
232	ASP	253	ILE	9.673	9.146	0.528	0.528
238	VAL	260	LEU	2.867	3.395	-0.528	0.528
636	CYS	666	PHE	3.998	3.471	0.527	0.527
429	LEU	433	VAL	0.000	0.526	-0.526	0.526
29	CYS	41	CYS	5.046	5.571	-0.524	0.524
284	CYS	408	CYS	7.300	6.778	0.523	0.523
498	GLY	563	TYR	11.066	10.544	0.523	0.523
83	VAL	108	ILE	2.590	3.112	-0.523	0.523
153	PHE	203	ASP	0.000	0.523	-0.523	0.523
221	PHE	245	GLY	3.144	2.621	0.522	0.522
489	GLN	566	VAL	1.277	1.799	-0.522	0.522
170	ASN	196	ALA	2.935	3.457	-0.522	0.522
29	CYS	39	VAL	0.973	0.451	0.522	0.522
523	ILE	604	ILE	1.337	0.815	0.521	0.521
674	ASN	679	ALA	0.520	0.000	0.520	0.520
141	PHE	158	ILE	1.156	1.674	-0.518	0.518
80	ARG	105	HIS	3.315	3.832	-0.518	0.518
597	VAL	633	ASP	0.517	0.000	0.517	0.517
409	GLU	477	THR	0.000	0.515	-0.515	0.515
189	PHE	212	TYR	3.733	4.244	-0.511	0.511
217	ASP	242	PRO	2.179	1.667	0.511	0.511
481	TYR	485	ALA	2.420	1.910	0.511	0.511
293	ARG	394	GLU	0.510	0.000	0.510	0.510
191	SER	193	GLN	0.946	0.437	0.509	0.509
254	ALA	277	LEU	3.005	3.514	-0.509	0.509
466	TYR	570	LEU	0.867	0.359	0.508	0.508
57	LEU	80	ARG	3.222	3.729	-0.507	0.507
194	GLY	217	ASP	4.354	4.861	-0.507	0.507
2	ARG	3	PRO	0.644	1.150	-0.506	0.506
133	ILE	157	GLU	3.030	3.533	-0.504	0.504
195	TYR	219	ASP	5.247	5.749	-0.502	0.502
510	TYR	541	ILE	4.216	4.718	-0.502	0.502
410	ASP	477	THR	0.772	0.270	0.502	0.502
424	VAL	668	PRO	1.395	1.896	-0.501	0.501
382	ASP	383	SER	0.000	0.501	-0.501	0.501
70	HIS	96	SER	0.543	1.044	-0.500	0.500
67	ILE	93	GLU	4.415	4.912	-0.497	0.497
142	PRO	158	ILE	0.654	0.158	0.496	0.496
459	ALA	508	SER	1.988	1.493	0.495	0.495
568	ILE	664	VAL	0.000	0.495	-0.495	0.495
173	GLN	198	ASN	1.603	2.098	-0.494	0.494
615	PRO	620	THR	0.000	0.494	-0.494	0.494
104	THR	130	PHE	3.136	2.646	0.491	0.491
252	LEU	275	ALA	2.100	2.591	-0.491	0.491
497	ALA	569	CYS	0.825	0.335	0.490	0.490
550	PHE	554	LEU	0.839	0.352	0.487	0.487
148	TYR	175	LEU	2.262	1.776	0.486	0.486
433	VAL	461	PHE	0.885	1.370	-0.486	0.486
502	VAL	563	TYR	2.130	2.616	-0.485	0.485
81	ILE	97	PHE	2.132	2.616	-0.484	0.484
635	ILE	669	LEU	0.484	0.000	0.484	0.484
31	CYS	37	PHE	0.583	1.066	-0.483	0.483
235	GLN	256	ASN	2.506	2.024	0.482	0.482
257	THR	279	TYR	0.033	0.515	-0.482	0.482
684	ALA	687	ARG	0.064	0.546	-0.481	0.481
53	SER	77	ASN	1.190	0.709	0.481	0.481
189	PHE	213	LEU	3.940	4.421	-0.480	0.480
18	ASP	19	LEU	0.000	0.480	-0.480	0.480
90	GLN	113	ASN	3.050	3.530	-0.480	0.480
79	SER	102	LYS	3.073	3.550	-0.477	0.477
210	ASN	236	THR	3.787	4.262	-0.475	0.475
129	LYS	153	PHE	1.923	1.449	0.474	0.474
432	ASN	671	SER	2.447	1.974	0.472	0.472
465	MET	500	PHE	0.000	0.471	-0.471	0.471
162	PRO	188	GLY	3.849	4.319	-0.470	0.470
711	VAL	712	PRO	0.308	0.778	-0.470	0.470
144	LEU	174	GLY	0.873	0.404	0.469	0.469
524	THR	607	THR	0.341	0.810	-0.469	0.469
208	ASN	233	VAL	0.756	0.287	0.468	0.468
181	THR	203	ASP	0.502	0.970	-0.468	0.468
349	ASN	351	HIS	0.554	0.087	0.467	0.467
126	PRO	149	SER	3.899	3.433	0.466	0.466
236	THR	238	VAL	2.298	2.764	-0.465	0.465
629	LEU	674	ASN	0.000	0.465	-0.465	0.465
583	ILE	648	ILE	0.872	0.407	0.465	0.465
64	LEU	86	ASP	7.898	7.435	0.463	0.463
180	LEU	203	ASP	2.498	2.961	-0.463	0.463
590	ASN	637	MET	0.462	0.000	0.462	0.462
470	ILE	568	ILE	1.623	1.163	0.460	0.460
28	PRO	44	ILE	0.990	1.448	-0.458	0.458
329	ASN	330	LEU	0.457	0.000	0.457	0.457
90	GLN	91	GLN	0.457	0.000	0.457	0.457
114	LEU	117	ILE	0.252	0.709	-0.457	0.457
107	GLU	130	PHE	1.481	1.025	0.457	0.457
136	THR	163	TYR	0.104	0.560	-0.456	0.456
448	VAL	534	ARG	0.813	0.359	0.455	0.455
159	THR	187	ASN	4.384	3.929	0.454	0.454
39	VAL	51	PRO	0.625	0.171	0.454	0.454
435	VAL	678	TYR	0.000	0.454	-0.454	0.454
202	LEU	229	SER	0.333	0.787	-0.454	0.454
494	CYS	569	CYS	8.679	8.226	0.453	0.453
411	ILE	470	ILE	0.705	0.253	0.452	0.452
668	PRO	671	SER	0.799	0.348	0.451	0.451
30	GLU	39	VAL	2.893	2.442	0.450	0.450
159	THR	186	ASN	0.058	0.507	-0.449	0.449
565	LYS	572	MET	1.110	0.661	0.448	0.448
402	SER	406	ASN	0.448	0.000	0.448	0.448
91	GLN	116	TYR	4.225	3.780	0.445	0.445
231	LEU	249	LEU	1.663	2.107	-0.444	0.444
35	GLU	55	GLN	0.445	0.001	0.444	0.444
233	VAL	253	ILE	3.626	4.069	-0.443	0.443
210	ASN	235	GLN	0.909	1.351	-0.442	0.442
182	LEU	184	LEU	0.830	0.389	0.442	0.442
519	ARG	626	MET	0.000	0.442	-0.442	0.442
39	VAL	58	LYS	3.559	3.998	-0.439	0.439
415	LYS	418	ARG	0.000	0.439	-0.439	0.439
502	VAL	553	ALA	2.535	2.097	0.439	0.439
520	TRP	600	CYS	2.387	2.826	-0.439	0.439
202	LEU	224	VAL	2.021	1.583	0.438	0.438
477	THR	481	TYR	1.896	2.333	-0.437	0.437
605	TYR	626	MET	0.436	0.000	0.436	0.436
260	LEU	279	TYR	1.642	2.078	-0.436	0.436
290	LYS	396	MET	0.436	0.000	0.436	0.436
432	ASN	461	PHE	3.112	2.677	0.435	0.435
608	VAL	613	TYR	0.000	0.435	-0.435	0.435
507	LEU	542	MET	0.435	0.000	0.435	0.435
195	TYR	198	ASN	1.024	0.589	0.435	0.435
449	PRO	519	ARG	0.000	0.435	-0.435	0.435
41	CYS	48	PRO	0.000	0.432	-0.432	0.432
438	ILE	450	ARG	0.432	0.000	0.432	0.432
566	VAL	570	LEU	0.068	0.499	-0.431	0.431
637	MET	667	TYR	0.000	0.431	-0.431	0.431
157	GLU	159	THR	0.568	0.998	-0.430	0.430
24	CYS	51	PRO	1.769	1.339	0.430	0.430
165	THR	166	SER	0.193	0.621	-0.428	0.428
265	LEU	482	TYR	0.427	0.000	0.427	0.427
111	THR	114	LEU	2.701	2.274	0.427	0.427
117	ILE	122	LEU	0.426	0.000	0.426	0.426
108	ILE	132	GLY	4.125	4.549	-0.424	0.424
221	PHE	228	PRO	1.653	2.077	-0.424	0.424
234	SER	255	ARG	9.427	9.006	0.421	0.421
405	PHE	406	ASN	0.567	0.146	0.421	0.421
512	LEU	516	THR	0.000	0.420	-0.420	0.420
302	ASN	305	SER	0.000	0.420	-0.420	0.420
192	VAL	217	ASP	2.554	2.973	-0.419	0.419
576	THR	578	LEU	0.419	0.000	0.419	0.419
556	PRO	562	SER	0.325	0.741	-0.416	0.416
499	PHE	563	TYR	4.260	3.845	0.416	0.416
151	ASP	176	CYS	1.478	1.064	0.414	0.414
417	LEU	664	VAL	1.107	1.519	-0.412	0.412
188	GLY	212	TYR	0.202	0.612	-0.410	0.410
172	PHE	200	THR	1.819	2.229	-0.410	0.410
232	ASP	234	SER	3.782	3.372	0.410	0.410
410	ASP	480	GLU	0.410	0.000	0.410	0.410
214	THR	237	SER	4.196	4.605	-0.409	0.409
73	SER	100	LEU	0.553	0.145	0.408	0.408
190	THR	212	TYR	4.047	3.640	0.407	0.407
125	LEU	149	SER	1.768	2.175	-0.407	0.407
117	ILE	142	PRO	0.147	0.554	-0.406	0.406
142	PRO	144	LEU	1.222	0.817	0.405	0.405
71	ALA	72	PHE	2.453	2.049	0.404	0.404
520	TRP	603	LYS	2.500	2.903	-0.404	0.404
207	LEU	216	ILE	0.535	0.132	0.403	0.403
243	SER	264	PRO	0.000	0.402	-0.402	0.402
158	ILE	164	MET	1.089	0.687	0.402	0.402
125	LEU	147	VAL	4.207	4.608	-0.401	0.401
459	ALA	507	LEU	2.531	2.132	0.399	0.399
573	ASP	575	GLU	1.251	1.648	-0.397	0.397
95	HIS	120	ASP	4.348	4.745	-0.397	0.397
29	CYS	50	LEU	0.452	0.056	0.396	0.396
211	LYS	235	GLN	1.271	1.667	-0.396	0.396
57	LEU	82	TYR	4.308	4.704	-0.396	0.396
555	LEU	561	SER	0.095	0.489	-0.394	0.394
65	ARG	88	THR	2.017	2.411	-0.394	0.394
374	GLN	375	GLU	0.000	0.393	-0.393	0.393
254	ALA	257	THR	0.103	0.495	-0.392	0.392
516	THR	597	VAL	3.410	3.019	0.391	0.391
314	SER	315	VAL	0.123	0.513	-0.390	0.390
278	SER	400	PRO	1.291	0.901	0.390	0.390
296	LEU	300	MET	0.389	0.000	0.389	0.389
601	TYR	604	ILE	0.388	0.000	0.388	0.388
141	PHE	184	LEU	0.388	0.000	0.388	0.388
442	SER	450	ARG	2.724	2.336	0.388	0.388
108	ILE	111	THR	1.601	1.988	-0.387	0.387
458	PHE	503	PHE	0.363	0.750	-0.387	0.387
29	CYS	48	PRO	1.402	1.015	0.387	0.387
138	LEU	164	MET	3.822	3.436	0.385	0.385
123	LYS	146	LYS	3.947	4.332	-0.385	0.385
89	LEU	92	LEU	0.236	0.621	-0.385	0.385
37	PHE	53	SER	4.194	3.809	0.384	0.384
277	LEU	282	HIS	2.410	2.027	0.383	0.383
446	LEU	450	ARG	1.398	1.015	0.383	0.383
47	ILE	59	LEU	0.382	0.000	0.382	0.382
139	LYS	163	TYR	4.636	4.255	0.382	0.382
47	ILE	50	LEU	0.695	0.314	0.382	0.382
539	CYS	542	MET	0.000	0.382	-0.382	0.382
97	PHE	121	ALA	5.808	6.188	-0.380	0.380
42	LYS	62	THR	0.379	0.000	0.379	0.379
512	LEU	674	ASN	0.379	0.000	0.379	0.379
103	VAL	128	LEU	3.867	3.489	0.378	0.378
107	GLU	134	PHE	1.062	1.439	-0.377	0.377
260	LEU	263	LEU	0.265	0.640	-0.375	0.375
279	TYR	281	SER	0.293	0.667	-0.374	0.374
640	ILE	666	PHE	0.374	0.000	0.374	0.374
124	GLU	148	TYR	0.016	0.388	-0.372	0.372
523	ILE	607	THR	0.732	1.104	-0.372	0.372
164	MET	188	GLY	3.984	3.613	0.371	0.371
646	SER	652	PRO	0.465	0.097	0.368	0.368
218	LYS	242	PRO	1.889	2.256	-0.366	0.366
81	ILE	107	GLU	3.084	3.450	-0.366	0.366
421	VAL	422	TRP	0.365	0.000	0.365	0.365
78	ILE	102	LYS	3.604	3.969	-0.364	0.364
55	GLN	80	ARG	2.525	2.888	-0.364	0.364
192	VAL	215	VAL	2.512	2.148	0.363	0.363
221	PHE	244	LYS	1.153	0.791	0.362	0.362
568	ILE	572	MET	0.362	0.000	0.362	0.362
251	GLU	272	LEU	1.131	0.771	0.360	0.360
455	ASN	546	TRP	3.088	3.447	-0.359	0.359
566	VAL	656	VAL	0.359	0.000	0.359	0.359
405	PHE	659	SER	0.000	0.358	-0.358	0.358
54	THR	78	ILE	2.603	2.960	-0.357	0.357
570	LEU	571	PRO	0.937	1.293	-0.357	0.357
205	VAL	230	LEU	3.647	4.002	-0.355	0.355
661	ILE	666	PHE	0.373	0.020	0.353	0.353
634	PHE	639	PRO	0.705	0.353	0.352	0.352
254	ALA	278	SER	4.452	4.100	0.352	0.352
180	LEU	182	LEU	2.423	2.774	-0.350	0.350
513	THR	596	ILE	0.673	0.323	0.350	0.350
498	GLY	569	CYS	2.495	2.844	-0.349	0.349

RRCS change distribution

0.11

Mean ΔRRCS

1.60

Std Dev

0.02

Median

Magnitude classification

High (|Δ| ≥ 5.0)

162

Medium (2.2 ≤ |Δ| < 5.0)

1663

Low (|Δ| < 2.2)

Methods

RRCS analysis. Residue-Residue Contact Scores (RRCS) were calculated for each conformational state based on inter-atomic distances. Changes (ΔRRCS) were computed by comparing active and inactive structures predicted by AlphaFold multistate (del Alamo et al., 2022).

Significance threshold. |ΔRRCS| ≥ 2.22, computed as max(mean(|Δ|) + σ, 0.2). The 0.2 floor ensures receptors with very small overall change still surface their largest contacts.

Variant data. Population frequencies from gnomAD v4; pathogenicity predictions from AlphaMissense; conservation from ProtVar / UniProt.

Structural annotation. TM domain boundaries and generic numbering from GPCRdb.

What is RRCS?

Residue-Residue Contact Score (RRCS) measures how strongly two amino acids interact in a protein structure. When a protein changes shape (from inactive to active), these contact strengths change.

ΔRRCS (Delta RRCS) shows the difference in contact strength between states:

Positive ΔRRCS. Contact is stronger in the active state.
Negative ΔRRCS. Contact is stronger in the inactive state.
Large |ΔRRCS|. Significant structural rearrangement.

Residues with large RRCS changes are critical for protein function. Mutations at these positions may disrupt the protein's ability to change shape properly.

Pathogenicity predictions

AlphaMissense predicts whether a mutation harms protein function:

Pathogenic (score ≥ 0.564): mutation likely damages function.
Ambiguous (0.34–0.563): effect uncertain.
Benign (< 0.34): mutation likely tolerated.

Conservation & population data

Conservation score. How well-preserved a position is across species (0 = variable, 1 = conserved). High conservation suggests the position is critical for function.

Allele frequency. How often a variant appears in the population. Rare variants (low frequency) may be more likely to be harmful.

Sources: AlphaFold multistate · RRCS · gnomAD v4 · AlphaMissense · GPCRdb · UniProt / ProtVar